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Efficient induction of differentiation of ES cells encapsulated in cytocompatible polymer gel and understanding its mechanism

Research Project

Project/Area Number 26282135
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionThe University of Tokyo

Principal Investigator

ISHIHARA KAZUHIKO  東京大学, 工学(系)研究科(研究院), 教授 (90193341)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywordsポリマーハイドロゲル / 細胞親和性 / 細胞機能制御 / ES細胞 / リン脂質ポリマー / 分化誘導 / 細胞固定化 / 細胞周期 / 自発形成ハイドロゲル / 高効率分化誘導 / 細胞ソースの均質化 / MPCポリマー / 細胞外マトリックス / 細胞親和型ポリマーハイドロゲル / 細胞分化誘導 / 弾性率 / バイオマテリアル / 細胞・組織 / ハイドロゲル
Outline of Final Research Achievements

Polymeric hydrogel matrix was prepared from a water-soluble phospholipid polymer (MPC polymer) with high cytocompatibility. It functioned as a fine artificial extracellular matrix (ECM) to control cell functions when the cells embedding in the MPC polymer hydrogel. This artificial ECM is reversibly dissociated by the addition of a low molecular weight sugar compound. The proliferation cycle of the ES cells embedding in the hydrogel was aligned with the G0/G1 phase, and it became clear that efficiency is several times multiplied by adding the differentiation-inducing factor in this state. We also succeeded in inducing aggregates derived from one cell in the matrix. Innovative cell manipulation method/development of differentiation induction method can be developed, and along with establishment of iPS cell and ES cell preparation method, it will be developed as fundamental technology for future cell engineering and tissue regeneration medicine.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (20 results)

All 2017 2016 2015 2014 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 6 results) Presentation (11 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Efficient differentiation of stem cells encapsulated in a cytocompatible phospholipid polymer hydrogel with tunable physical properties2015

    • Author(s)
      H.Oda, T.Konno, K.Ishihara
    • Journal Title

      Biomaterials

      Volume: 36 Pages: 86-91

    • DOI

      10.1016/j.biomaterials.2015.03.051

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Bioinspired phospholipid polymer hydrogel system for cellular engineering2015

    • Author(s)
      K.Ishihara, H.Oda, T.Aikawa, T.Konno
    • Journal Title

      Macromol Symp

      Volume: 351 Issue: 1 Pages: 69-77

    • DOI

      10.1002/masy.201300118

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cytocompatible and spontaneous forming phospholipid polymer hydrogels2015

    • Author(s)
      B.Gao, T.Konno, K.Ishihara
    • Journal Title

      Eur Polym J

      Volume: 72 Pages: 577-589

    • DOI

      10.1016/j.eurpolymj.2015.03.030

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cytocompatible and spontaneous forming phospholipid polymer hydrogels2015

    • Author(s)
      Botao Gao, Tomohiro Konno, and Kazuhiko Ishihara
    • Journal Title

      European Polymer Journal

      Volume: 67 Pages: 1-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Bioinspired phospholipid polymer hydrogel system for cellular engineering2015

    • Author(s)
      Kazuhiko Ishihara, Haruka Oda, Tatsuo Aikawa, Tomohiro Konno
    • Journal Title

      Macromolecular Symposia

      Volume: 28 Pages: 1-10

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cross-linkable and water-soluble phospholipid polymer as artificial extracellular matrix2014

    • Author(s)
      Eri Maeta, Kazuhiko Ishihara
    • Journal Title

      Biomaterials & Biomedical Engineering

      Volume: 1 Pages: 157-170

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Bioinspired polymer hydrogels for advanced cell engineering2016

    • Author(s)
      Kazuhiko Ishihara, Haruka Oda, Tomohiro Konno
    • Organizer
      The 1st International Conference of Molecular Engineering of Polymers (MEP-1)
    • Place of Presentation
      Shanghai
    • Year and Date
      2016-10-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional control of stem cells encapsulated in cytocompatible and reversible MPC polymer hydrogel matrix2016

    • Author(s)
      Kazuhiko Ishihara, Haruka Oda, Tomohiro Konno
    • Organizer
      10th World Biomaterials Congress
    • Place of Presentation
      Montreal
    • Year and Date
      2016-05-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cytocompatible, spontaneously forming and reversible dissociatable phospholipid polymer hydrogel system for regulating cellular functio2015

    • Author(s)
      K. Ishihara, H. Oda, T. Konno
    • Organizer
      Ludwing-Maximilians-Universitat Munchen(LMU)-University of Tokyo Symposium
    • Place of Presentation
      Munich (Germany)
    • Year and Date
      2015-11-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] リン脂質ポリマーバイオマテリアルの創製と先端医療への応用2015

    • Author(s)
      石原一彦
    • Organizer
      日本薬剤学会第40回製剤・創剤セミナー
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県・淡路市)
    • Year and Date
      2015-08-20
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 内包細胞の増殖制御を目指したリン脂質ポリマーハイドロゲルによる細胞周辺微小環境の構築2015

    • Author(s)
      小田悠加、金野智浩、石原一彦
    • Organizer
      第64回高分子学会年次大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2015-05-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Phenylboronic acid group-connecting block-type phospholipid polymers for spontaneously forming hydrogels2015

    • Author(s)
      Kazuhiko Ishihara, Surasak Chantasirichot, Yuuki Inoue
    • Organizer
      Frontiers in Polymer Science 2015
    • Place of Presentation
      Lake Garda (Italy)
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bioinspired phospholipid polymer matrix encapsulated with cells for regulating cellular functions2015

    • Author(s)
      Kazuhiko Ishihara, Haruka Oda
    • Organizer
      4th International Conference on Multifunctional, Hybrid and Nanomaterials
    • Place of Presentation
      Sitges, Spain
    • Year and Date
      2015-03-09 – 2015-03-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Microenvironment controllable 3-D MPC polymer hydrogels as cellular matrices2014

    • Author(s)
      Haruka Oda, Tomohiro Konno, Kazuhiko Ishihara
    • Organizer
      International Polymer Conference 2014
    • Place of Presentation
      Tsukuba, EPOCAL TSUKUBA
    • Year and Date
      2014-12-02 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] How do bioactive molecules affect cells immobilized in layer-by-layer phospholipid polymer hydrogels?2014

    • Author(s)
      Botao Gao, Tomohiro Konno, Kazuhiko Ishihara
    • Organizer
      International Polymer Conference 2014
    • Place of Presentation
      Tsukuba, EPOCAL TSUKUBA
    • Year and Date
      2014-12-02 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Functionalization of extremely biocompatible phospholipid polymers2014

    • Author(s)
      Kazuhiko Ishihara
    • Organizer
      Gordon Research Conference on Bioinspired Materials
    • Place of Presentation
      Newry, USA
    • Year and Date
      2014-06-22 – 2014-06-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Versatile ABA triblock Phospholipid polymer by ATRP for hydrogel with tunable mechanical properties2014

    • Author(s)
      Surasak Chantasirichot, Yuuki Inou1, Kazuhiko Ishihara
    • Organizer
      Macro 2014
    • Place of Presentation
      Chang Mai, Thailand
    • Year and Date
      2014-06-07 – 2014-06-10
    • Related Report
      2014 Annual Research Report
  • [Book] Handbook of Intelligent Scaffolds for Regenerative Medicine, 2nd Eds2017

    • Author(s)
      Kazuhiko Ishihara, Haruka Oda, Tomohiro Konno
    • Total Pages
      1440
    • Publisher
      Taylor & Francis)
    • Related Report
      2016 Annual Research Report
  • [Remarks] 石原研究室

    • URL

      http://www.mpc.t.u-tokyo.ac.jp/

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Remarks] 石原研究室:研究テーマ

    • URL

      http://www.mpc.t.u-tokyo.ac.jp/research.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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