Mutual central-peripheral interaction in cooperation among sleep, exercise and metabolism

• Project/Area Number: 26282193
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: The University of Tokushima
• Principal Investigator: SEI Hiroyoshi 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (40206602)
• Co-Investigator(Kenkyū-buntansha): 近久 幸子 徳島大学, 大学院医歯薬学研究部(医学系), 講師 (00452649)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000); Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 睡眠 / 運動 / 代謝 / ケトン体 / 脳 / 肝臓 / HMGCS2 / 生理学 / 環境生理学 / ホメオスタシス / 脂質代謝

Outline of Final Research Achievements

The present study explored how ketone body metabolism in the central and liver (peripheral) respectively relates to sleep regulation. Experiments on suppression of HMGCS2 (ketone synthase) expression in the central and liver using siRNA oligos and establishment of HMGCS2 and SCOT (ketone degrading enzyme) expression regulating mice of neurons using the tet system were carried out. As a result, the possibility that the central HMGCS 2 is involved in the sleep-depth rebound after deprivation by handling and HMGCS 2 in the liver is involved in the sleep-depth rebound after treadmill exercise has come to light.

Research Products

• [Journal Article] Mast cell involvement in glucose tolerance impairment caused by chronic mild stress with sleep disturbance (2017)
  • Author(s): Chikahisa Sachiko、Harada Saki、Shimizu Noriyuki、Shiuchi Tetsuya、Otsuka Airi、Nishino Seiji、Sei Hiroyoshi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 13640-13640
  • DOI: 10.1038/s41598-017-14162-w
  • NAID: 120006535032
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 睡眠と生理機能 (2017)
  • Author(s): 勢井宏義
  • Organizer: 日本睡眠学会第42回定期学術集会（横浜）
• [Presentation] Short time running exercise enhances sleep pressure in mice (2016)
  • Author(s): 清水紀之、吉岡由布、近久幸子、木藤有紀、志内哲也、勢井宏義
  • Organizer: Neuroscience 2016
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] トレッドミルによる一過性の短時間運動に伴う睡眠への影響とケトン代謝との関連性 (2016)
  • Author(s): 清水紀之、吉岡由布、三崎貴文、木藤有紀、近久幸子、志内哲也、勢井宏義
  • Organizer: 日本睡眠学会第41回定期学術集会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2016-07-07
• [Presentation] 短時間運動は時間依存的に睡眠深度を増大させる (2016)
  • Author(s): 清水紀之、吉岡由、三崎貴文、近久幸子、志内哲也、勢井宏義
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-03-22
• [Presentation] Contribution of PPARα and Ketone Body to Sleep Homeostasis in Mice. (2015)
  • Author(s): S. Chikahisa, N. Shimizu, T. Shiuchi and H. Sei.
  • Organizer: Experimental Biology 2015
  • Place of Presentation: Boston, Massachusetts, USA
  • Year and Date: 2015-03-28 – 2015-04-01
• [Presentation] Exercise Enhances Following Sleep in Mice. (2015)
  • Author(s): Y. Yoshioka, S. Chikahisa, N. Shimizu, T. Shiuchi and H. Sei.
  • Organizer: Experimental Biology 2015
  • Place of Presentation: Boston, Massachusetts, USA
  • Year and Date: 2015-03-28 – 2015-04-01