Selective detection and stabilization of G-quadruplexes in genome using G4 ligands
| Project/Area Number |
26282214
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Nagasawa Kazuo 東京農工大学, 工学(系)研究科(研究院), 教授 (10247223)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | グアニン四重鎖 / テロメア / 大環状ポリオキサゾール / ビオチンプローブ / G４アフィニティカラム / トポロジー / テロメスタチン / ポリオキサゾール / グアニン４重鎖 / 化学プローブ / プルダウン / 探索 / Pull-down |
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| Outline of Final Research Achievements |
G-quadruplex (G4), a higher order structure of DNA, is involved in transcriptional regulation of genes, and detection of G4s in genome leads to the discoveries of new drug targets. Furthermore, selective stabilization of each G4 would become the selective control of the corresponding G4 function. Based on our macrocyclic polyoxazole type G4 ligand (6OTD, 7OTD), we have developed a new 7OTD analog attached with biotin-function. With the new ligand, we prepared a G4 affinity column, which enable us to detect the G4-forming DNA sequences in genome. In addition, we have developed new 6OTD derivatives for stabilizing two out of three representative topologies in G4 selectively.
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Report
(5 results)
Research Products
(114 results)
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[Journal Article] Targeting glioma stem cells in vivo by a G-quadruplex-stabilizing synthetic macrocylic hexaoxazole2017
Author(s)
Nakamura T, Okabe S, Yoshida H, Iida K, Ma Y, Sasaki S, Yamori T, Shin-Ya K, Nakano I, Nagasawa K, Seimiya H.
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Journal Title
Sci Rep.
Volume: 7
Pages: 3605-3605
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Time-resolved crystal structures of the reaction intermediate of nitrile hydratase reveal a role for the cysteine-sulfenic acid ligand as a catalytic nucleophile2015
Author(s)
Y. Yamanaka, Y. Kato, K. Hashimoto, K. Iida, K. Nagasawa, H. Nakayama, N. Dohmae, K. Noguchi, T. Noguchi, M. Yohda, M. Odaka
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Journal Title
Angew. Chem. Int. Ed.
Volume: 54
Pages: 10763-10767
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Rif1 binds to G-quadruplexes and suppresses replication over long distances.2015
Author(s)
Yutaka Kanoh, Seiji Matsumoto, Rino Fukatsu, Naoko Kakusho, Nobuaki Kono, Claire Renard-Guillet, Koji Masuda, Keisuke Iida, Kazuo Nagasawa, Katsuhiko Shirahige, and *Hisao Masai
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Journal Title
Nat Struct Mol Biol
Volume: 22
Pages: 889-897
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] 貝毒サキシトキシン類の化学2015
Author(s)
長澤和夫
Organizer
第32回有機合成化学セミナー
Place of Presentation
ニューウェルシティ湯河原（静岡県・熱海市）
Year and Date
2015-09-15
Related Report
Invited
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[Presentation] パラウアミンの合成研究2015
Author(s)
岩田真、今岡拓哉、松尾浩喜、長澤和夫
Organizer
第69回有機合成化学協会関東支部シンポジウム
Place of Presentation
横浜国立大学（神奈川県・横浜市）
Year and Date
2015-05-16
Related Report
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