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Imaging biomolecular dynamics verified using fluorogenic probes

Research Project

Project/Area Number 26282215
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Chemical biology
Research InstitutionOsaka University

Principal Investigator

Hori Yuichiro  大阪大学, 工学研究科, 准教授 (00444563)

Research Collaborator NISHIURA Miyako  
BABA Reisuke  
HIRAYAMA Shinya  
OTOMURA Norimichi  
TAO Tomomi  
GAO Jingchi  
NISHIDA Ayuko  
UMENO Maho  
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords発蛍光プローブ / GLUT4 / DNAメチル化 / PYPタグ / ハイブリッドプローブ / メチル化DNA / 細胞周期 / 機能性超分子プローブ / Regnase-1
Outline of Final Research Achievements

Imaging of biomolecules is a useful method to elucidate biological phenomena in living cells. So far, fluorescent proteins have been used for live-cell imaging of proteins. In this research, to overcome limitations of existing methods, we developed fluorogenic probes, which emit fluorescence upon binding to biomolecules. By using the fluorogenic probes, we succeeded in analyzing protein trafficking, glycan function, and chemical modification of nucleic acids.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (14 results)

All 2018 2017 2016 2015 2014

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results,  Acknowledgement Compliant: 4 results) Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 6 results)

  • [Journal Article] Synthetic-Molecule/Protein Hybrid Probe with Fluorogenic Switch for Live-Cell Imaging of DNA Methylation2018

    • Author(s)
      Yuichiro Hori, Norimichi Otomura, Ayuko Nishida, Miyako Nishiura, Maho Umeno, Isao Suetake, Kazuya Kikuchi
    • Journal Title

      J Am Chem Soc

      Volume: 140 Issue: 5 Pages: 1686-1690

    • DOI

      10.1021/jacs.7b09713

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of cyanine probes with dinitrobenzene quencher for rapid fluorogenic protein labelling2017

    • Author(s)
      Yuichiro Hori, Shinya Hirayama, Kazuya Kikuchi
    • Journal Title

      Philos Trans A Math Phys Eng Sci

      Volume: 375 Issue: 2107 Pages: 20170018-20170018

    • DOI

      10.1098/rsta.2017.0018

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorogenic Probes Reveal a Role of GLUT4 N-glycosylation in Intracellular Trafficking2016

    • Author(s)
      S. Hirayama, Y. Hori, Z. Benedek, T. Suzuki, K. Kikuchi
    • Journal Title

      Nature Chemical Biology

      Volume: 12 Issue: 10 Pages: 853-859

    • DOI

      10.1038/nchembio.2156

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Design of a Protein tag and Fluorogenic Probe with Modular Structure for Live-Cell Imaging of Intracellular Proteins2016

    • Author(s)
      Y. Kamikawa, Y. Hori, K. Yamashita, L. Jin, S. Hirayama, D.M. Standley & K. Kikuchi
    • Journal Title

      Chemical Science

      Volume: 7 Issue: 1 Pages: 308-314

    • DOI

      10.1039/c5sc02351c

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of Fluorogenic Probes for the Detection of Histone Deacetylase Activity2015

    • Author(s)
      堀 雄一郎、馬場 玲輔、菊地 和也
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 135 Issue: 1 Pages: 23-29

    • DOI

      10.1248/yakushi.14-00202-3

    • NAID

      130004756464

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2015-01-01
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Redesign of Fluorogenic Labeling System to Improve Surface Charges, Brightness, and Binding Kinetics for Imaging Functional Localization of Bromodomains2015

    • Author(s)
      Y. Hori, S. Hirayama, M. Sato & K. Kikuchi
    • Journal Title

      Angewante Chemie International Edition

      Volume: 54 Issue: 48 Pages: 14368-14371

    • DOI

      10.1002/anie.201506935

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Intramolecular Long-distance Nucleophilic Reactions as a Rapid Fluorogenic Switch Applicable to Detection of Enzymatic Activity2015

    • Author(s)
      Reisuke BABA, Yuichiro HORI, Kazuya KIKUCHI
    • Journal Title

      Chem. Eur. J.

      Volume: 21 Issue: 12 Pages: 4695-4702

    • DOI

      10.1002/chem.201406093

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Chemical probes with fluorogenic switch for imaging modified protein and DNA2017

    • Author(s)
      Yuichiro Hori
    • Organizer
      ISBC 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 合成分子と蛋白質を駆使した生体分子イメージング2017

    • Author(s)
      堀 雄一郎
    • Organizer
      公益財団法人サントリー生命科学財団 生有研シンポジウム ユニークな生命現象の解明を目指した「新しい鍵化合物」の創製
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Chemical probes with fluorogenic switches for visualizing modified protein and DNA2016

    • Author(s)
      Yuichiro Hori
    • Organizer
      BSJ-BSC Joint Symposium for Protein Design and its Applications to Biotechnology at the 54th Annual Meeting of the Biophysical Society of Japan (BSJ)
    • Place of Presentation
      Tsukuba, Japan
    • Year and Date
      2016-12-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Chemical probes for visualizing biological phenomena from glycobiology to epigenetics2016

    • Author(s)
      Yuichiro Hori
    • Organizer
      IPR seminar: Frontiers of peptide and glycoscience
    • Place of Presentation
      Suita, Japan
    • Year and Date
      2016-05-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of protein-labeling probes with fluorogenic switches for imaging cellular events2015

    • Author(s)
      Yuichiro Hori
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Honolulu, U.S.A.
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescent Probes for in vivo Imaging and Epigenetic Analysis2015

    • Author(s)
      Yuichiro Hori
    • Organizer
      Asian International Symposium -Natural Products Chemistry, Chemical Biology / Biofunctional Chemistry and Biotechnology-
    • Place of Presentation
      Funabashi, Japan
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of Chemical Tools for Imaging Protein Localization and Epigenetic Phenomena2014

    • Author(s)
      Yuichiro Hori, Norimichi Otomura, Reisuke Baba, Kazuya Kikuchi
    • Organizer
      wiss-Japanese Chemical Biology Symposium 2014
    • Place of Presentation
      Bern, Switzerland
    • Year and Date
      2014-10-02 – 2014-10-03
    • Related Report
      2014 Annual Research Report
    • Invited

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Published: 2014-04-04   Modified: 2019-03-29  

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