Dynamics analysis of biomokecules using fluorescence diamond nanoparticles
Project/Area Number |
26286028
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Nanobioscience
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Research Institution | Osaka University (2016) Kyoto University (2014-2015) |
Principal Investigator |
Harada Yoshie 大阪大学, 蛋白質研究所, 教授 (10202269)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
|
Keywords | ナノバイオ / 生物物理学 / 生物物理 |
Outline of Final Research Achievements |
We have developed a device and computer programs to measure and analyze ODMR spectra of NV centers in diamond based on an inverted fluorescence microscope, and succeeded in determining the precise orientation of each diamond nanoparticle by applying external magnetic fields sequentially in four inequivalent directions. Diamond nanoparticles were specifically labeled on the EGF receptor via anti-EGF receptor antibody in the cell membrane of A431 cells. By measuring the ODMR spectrum every few minutes and determining the orientation of the diamond nanoparticles, it has become possible to detect the movement of the EGF receptor, that is, the fluctuation motion of the cell membrane. From now on, we will develop this technology to measure various biomolecular dynamics.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues.2015
Author(s)
Oomoto, I., Suzuki-Hirano, A., Umeshima, H., Han, YW., Yanagisawa, H., Carlton, P., Harada, Y., Kengaku, M., Okamoto, A., Shimogori, T. and Wang, DO.
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Journal Title
Nucleic Acids Res.
Volume: 43(19)
Issue: 19
Pages: e126-e126
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Synergistic effect of ATP for RuvA-RuvB-Holliday junction DNA complex formation.2015
Author(s)
Iwasa, T., Han, Y. W., Hiramatsu, R., Yokota, H., Nakao, K., Yokokawa, R., Ono, T., Harada, Y.
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Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 18177-18177
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Optically Detected Magnetic Resonance of Nanodiamonds In Vivo; Implementation of Selective Imaging and Fast Sampling.2015
Author(s)
Yoshinari, Y., Mori, S., Igarashi, R., Sugi, T., Yokota, H., Ikeda, K., Sumiya, H., Mori, I, Tochio, H., *Harada, Y. and Shirakawa, M.
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Journal Title
J Nanosci Nanotechnol.
Volume: 15
Issue: 2
Pages: 1014-1021
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comprehensive and quantitative analysis for controlling the physical/chemical states and particle properties of nanodiamonds for biological applications2015
Author(s)
Sotoma, S., Akagi, K., Hosokawa, S., Igarashi, R., Tochio, H., Harada, Y., and Shirakawa
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Journal Title
RSC Advances
Volume: 5
Issue: 18
Pages: 13818-13827
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Real-time single-cell imaging of protein secretion2014
Author(s)
Yamagishi, M. Suzuki, N. Izawa, K. Nakahara, A. Mizuno, J. Shoji, S. Heike, T. Harada, Y. Nishikomori, R. Ohara, O.
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Journal Title
Sci Rep
Volume: 4
Issue: 1
Pages: 4736-4736
DOI
Related Report
Peer Reviewed / Open Access
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