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Super resolution Raman microscopy using structured illumination

Research Project

Project/Area Number 26286063
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Optical engineering, Photon science
Research InstitutionOsaka University

Principal Investigator

Fujita Katsumasa  大阪大学, 工学(系)研究科(研究院), 准教授 (80362664)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Keywordsラマン顕微鏡 / ラマン分光 / 超解像顕微鏡 / 構造化照明 / ラマン散乱 / 光学顕微鏡
Outline of Final Research Achievements

A structured illumination Raman microscopy has been developed in order to realize a label-free analytical imaging technique with an improved spatial resolution. The light illumination optics has been newly designed and implemented to a line illumination Raman microscopy. The introduction of structured pattern into the line illumination for Raman scattering detection provided about a twice improvement of the spatial resolution in practical conditions, which corresponds about 1.5-fold improvement from the theoretical limit. The improvement of the spatial resolution has been confirmed by observing polymer nanoparticles ( a mixture of polystyrene and PMMA), carbon nanomaterials (CVD graphene and graphite), biological tissue (fibrous structure of lipid) samples.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (47 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (5 results) Journal Article (8 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (30 results) (of which Int'l Joint Research: 14 results,  Invited: 25 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] イエナ大学(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 国立台湾大学(台湾)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] ストラスクライド大学(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] イエナ大学(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 国立台湾大学(台湾)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Protein expression guided chemical profiling of living cells by hybrid fluorescence-Raman microscopy2017

    • Author(s)
      Liang-da Chiu , Taro Ichimura , Takumasa Sekiya , Hiroaki Machiyama , Tomonobu Watanabe , Hideaki Fujita , Takeaki Ozawa, Katsumasa Fujita
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 43569-43569

    • DOI

      10.1038/srep43569

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Non-label immune cell state prediction using Raman spectroscopy2016

    • Author(s)
      Taro Ichimura, Liang-da Chiu, Katsumasa Fujita, Hiroaki Machiyama, Tomoyuki Yamaguchi, Tomonobu M. Watanabe, and Hideaki Fujita
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 37562-37562

    • DOI

      10.1038/srep37562

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 構造化照明顕微鏡による高解像ラマンイメージング2016

    • Author(s)
      渡辺 梢, 藤田克昌
    • Journal Title

      レーザー研究

      Volume: 44 Pages: 648-652

    • NAID

      130007957249

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Follow-up review: Recent progress in the development of super-resolution optical microscope2016

    • Author(s)
      Katsumasa Fujita
    • Journal Title

      Microscopy

      Volume: 65 Issue: 4 Pages: 275-281

    • DOI

      10.1093/jmicro/dfw022

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-speed Raman imaging of cellular processes2016

    • Author(s)
      Jun Ando, Almar F Palonpon, Mikiko Sodeoka, Katsumasa Fujita
    • Journal Title

      Current Opinion in Chemical Biology

      Volume: 33 Pages: 16-24

    • DOI

      10.1016/j.cbpa.2016.04.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structured line illumination Raman microscopy2015

    • Author(s)
      Watanabe K, Palonpon AF, Smith NI, Chiu LD, Kasai A, Hashimoto H, Kawata S, Fujita K.
    • Journal Title

      Nat Commun.

      Volume: 6 Issue: 1 Pages: 10095-10095

    • DOI

      10.1038/ncomms10095

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 超解像光学顕微鏡の原理と医療応用に向けた課題2015

    • Author(s)
      藤田克昌
    • Journal Title

      光技術コンタクト

      Volume: 53 Pages: 31-36

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 光学顕微鏡2015

    • Author(s)
      藤田克昌
    • Journal Title

      病理と臨床

      Volume: 33 Pages: 531-539

    • Related Report
      2015 Annual Research Report
  • [Presentation] Raman microscopy for molecular imaging of biological samples2017

    • Author(s)
      Katsumasa Fujita
    • Organizer
      ICAVS-9
    • Place of Presentation
      Victoria, Canada
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Raman microscopy for molecular imaging of cells2017

    • Author(s)
      Katsumasa Fujita
    • Organizer
      Raman Fest
    • Place of Presentation
      West Lafayette, USA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Improvement of spatial and spectral resolution in Raman microscopy2017

    • Author(s)
      Katsumasa Fujita
    • Organizer
      Biomedical Imaging and Sensing Conference (BISC) 2017
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Raman microscopy for molecular imaging of living cells2017

    • Author(s)
      藤田克昌
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      アクトシティ浜松
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ラマン散乱顕微鏡における超解像2017

    • Author(s)
      藤田克昌
    • Organizer
      第64回応用物理学会春季学術講演会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Raman imaging of molecular dynamics during cellular events2017

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SPIE 2017 Nano-Bio Sensing, Imaging & Spectroscopy
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Structured illumination microscopy for vibrational molecular imaging2016

    • Author(s)
      Kozue Watanabe, Almar F. Palonpon, Nicholas I. Smith, Liang-Da Chiu, Atsushi Kasai, Hitoshi Hashimoto, Satoshi Kawata, Katsumasa Fujita
    • Organizer
      SPIE Optics + Photonics
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-08-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Toward Label-Free Super-Resolution Imaging with Raman Microscopy2016

    • Author(s)
      A.F. Palonpon, K. Watanabe, Y. Yonemaru, N.I. Smith, L.-d. Chiu, S. Kawano, A. Kasai, H. Hashimoto, S. Kawata, K. Fujita
    • Organizer
      Focus on Microscopy 2016
    • Place of Presentation
      Taipei
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Improvement of spatial- and temporal-resolution in Raman microscopy2016

    • Author(s)
      Katsumasa Fujita
    • Organizer
      Japan-Taiwan Medical Spectroscopy International Symposium (14th Annual Meeting of the Japan Association of Medical Spectroscopy)
    • Place of Presentation
      淡路夢舞台国際会議場
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ラマン散乱による細胞分析イメージング2016

    • Author(s)
      安藤潤, 藤田克昌
    • Organizer
      電子情報通信学会 第36回POC研究会
    • Place of Presentation
      産業技術総合研究所(池田市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ラマン散乱を利用した高解像度分子イメージング2016

    • Author(s)
      藤田克昌
    • Organizer
      日本光学会 ナノオプティクス研究グループ 第23回研究討論会
    • Place of Presentation
      大阪大学吹田キャンパス
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ラマン散乱を用いた顕微分析イメージング2016

    • Author(s)
      藤田克昌
    • Organizer
      高分子学会 高分子表面研究会
    • Place of Presentation
      東京理科大学
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ラマン散乱を用いた生体イメージング2016

    • Author(s)
      安藤潤, 藤田克昌
    • Organizer
      電気通信学会ソサイエティ大会
    • Place of Presentation
      北海道大学
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Raman miroscopy beyond the limit2016

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SCIX 2016
    • Place of Presentation
      Minneapolis, USA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ラマン顕微鏡:分光分析とイメージングとの融合2016

    • Author(s)
      藤田克昌
    • Organizer
      第4回バイオ関連化学シンポジウム若手フォーラム
    • Place of Presentation
      もてなしドーム地下イベント広場(金沢)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 顕微鏡の限界を超える:超解像顕微鏡とラマン散乱顕微鏡2016

    • Author(s)
      藤田克昌
    • Organizer
      組織細胞化学会総会
    • Place of Presentation
      杏林大学(東京)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 構造化ライン照明顕微鏡による高分解能ラマンイメージング2016

    • Author(s)
      渡辺梢, Almar F. Palonpon, Nicholas I. Smith, Liang-da Chiu, 笠井淳司, 橋本均, 河田 聡 , 藤田克昌
    • Organizer
      Optics & Photonics Japan
    • Place of Presentation
      筑波大学(東京)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Super-resolution microscopy using nonlinear light emission2015

    • Author(s)
      Katsumasa Fujita
    • Organizer
      Workshop on microscopy, biology, medicine, and advanced CMOS imagers
    • Place of Presentation
      Hamamatsu
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Raman microscopy: chemical and analytical imaging of biomolecules2015

    • Author(s)
      Katsumasa Fujita
    • Organizer
      IEEE CPMT Symposium Japan
    • Place of Presentation
      Kyoto
    • Year and Date
      2015-11-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Enhancement of the lateral resolution of Raman microscopy by use of structured illumination2015

    • Author(s)
      Almar F Palonpon, Kozue Watanabe, Nicholas Smith, Liang-da Chiu, Atsushi Kasai, Hitoshi Hashimoto, Satoshi Kawata, Katsumasa Fujita
    • Organizer
      JSAP-OSA Joint Symposia 2015
    • Place of Presentation
      Nagoya
    • Year and Date
      2015-09-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 超解像顕微鏡2015

    • Author(s)
      藤田克昌
    • Organizer
      第51回夏期セミナー
    • Place of Presentation
      幕張
    • Year and Date
      2015-09-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Super-resolution confocal microscopy using nonlinear optical emission2015

    • Author(s)
      Katsumasa Fujita
    • Organizer
      nternational Symposium on Surface Topography & Optical Microscopy 2015
    • Place of Presentation
      Harbin
    • Year and Date
      2015-07-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Chemical imaging of living cells by Raman microscopy2015

    • Author(s)
      Katsumasa Fujita
    • Organizer
      OSK Biophotonics symposium
    • Place of Presentation
      Gyeongju
    • Year and Date
      2015-07-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular imaging of cells by Raman scattering2015

    • Author(s)
      Katsumasa Fujita
    • Organizer
      Advanced Microscopy Meeting - Super Resolution in Different Dimensions
    • Place of Presentation
      Moscow, Russia
    • Year and Date
      2015-06-01 – 2015-06-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Molecular imaging of cells by using Raman scattering2015

    • Author(s)
      Katsumasa Fujita
    • Organizer
      第10回日本分子イメージング学会 学術集会
    • Place of Presentation
      タワーホール船堀, 東京
    • Year and Date
      2015-05-20 – 2015-05-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Raman microscopy for molecular imaging of cells2015

    • Author(s)
      Katsumasa Fujita
    • Organizer
      Applied Optics and Photonics, China
    • Place of Presentation
      Beijing, China
    • Year and Date
      2015-05-05 – 2015-05-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Raman microscopy using structured line illumination2015

    • Author(s)
      Kozue Watanabe, Almar F. Palonpon, Nicholas I. Smith, Liang-da Chiu, Atsushi Kasai, Hitoshi Hashimoto, Satoshi Kawata, and Katsumasa Fujita
    • Organizer
      Focus on Microscopy 2015
    • Place of Presentation
      Goettingen, Germany
    • Year and Date
      2015-03-29 – 2015-04-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] アルキン標識イメージング2015

    • Author(s)
      藤田克昌、安藤淳、袖岡幹子
    • Organizer
      日本分光学会赤外ラマン分光部会
    • Place of Presentation
      大阪大学、豊中市
    • Year and Date
      2015-01-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] High-resolution hyperspectral Raman imaging2015

    • Author(s)
      Kastsumasa Fujita, Kozue Watanabe, Jun Ando
    • Organizer
      The 45th Winter Colloquium on the Physics of Quantum Electronics
    • Place of Presentation
      Snowbird, USA
    • Year and Date
      2015-01-05 – 2015-01-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of a high-resolution and polarization-sensitive Raman microscope2014

    • Author(s)
      Katsumasa Fujita
    • Organizer
      SCIX2014
    • Place of Presentation
      Reno, USA
    • Year and Date
      2014-09-28 – 2014-10-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] 1分子ナノバイオ計測2014

    • Author(s)
      藤田克昌 (分担執筆)
    • Total Pages
      240
    • Publisher
      化学同人
    • Related Report
      2014 Annual Research Report
  • [Remarks] Katsumasa Fujita

    • URL

      http://lasie.ap.eng.osaka-u.ac.jp/ap1g1kat/index_j.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 藤田克昌

    • URL

      http://lasie.ap.eng.osaka-u.ac.jp/ap1g1kat/index_j.html

    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] ラマン顕微鏡及びラマン散乱光観察方法2014

    • Inventor(s)
      藤田克昌, 渡辺 梢, 河田 聡
    • Industrial Property Rights Holder
      大阪大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-165760
    • Filing Date
      2014-08-18
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2022-02-16  

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