Super resolution Raman microscopy using structured illumination
| Project/Area Number |
26286063
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Optical engineering, Photon science
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| Research Institution | Osaka University |
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Principal Investigator |
Fujita Katsumasa 大阪大学, 工学(系)研究科(研究院), 准教授 (80362664)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Keywords | ラマン顕微鏡 / ラマン分光 / 超解像顕微鏡 / 構造化照明 / ラマン散乱 / 光学顕微鏡 |
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| Outline of Final Research Achievements |
A structured illumination Raman microscopy has been developed in order to realize a label-free analytical imaging technique with an improved spatial resolution. The light illumination optics has been newly designed and implemented to a line illumination Raman microscopy. The introduction of structured pattern into the line illumination for Raman scattering detection provided about a twice improvement of the spatial resolution in practical conditions, which corresponds about 1.5-fold improvement from the theoretical limit. The improvement of the spatial resolution has been confirmed by observing polymer nanoparticles ( a mixture of polystyrene and PMMA), carbon nanomaterials (CVD graphene and graphite), biological tissue (fibrous structure of lipid) samples.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Protein expression guided chemical profiling of living cells by the simultaneous observation of Raman scattering and anti-Stokes fluorescence emission.2017
Author(s)
Chiu, L. Da, Ichimura, T., Sekiya, T., Machiyama, H., Watanabe, T., Fujita, H., Ozawa, T., & Fujita, K.
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Journal Title
Scientific Reports
Volume: 7
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] アルキン標識イメージング2015
Author(s)
藤田克昌、安藤淳、袖岡幹子
Organizer
日本分光学会赤外ラマン分光部会
Place of Presentation
大阪大学、豊中市
Year and Date
2015-01-27
Related Report
Invited
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