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Development of surface selective high-sensitivity fluorescent probe apparatus for microdroplets and its application to biomolecules

Research Project

Project/Area Number 26288010
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Physical chemistry
Research InstitutionKyushu University

Principal Investigator

Sekiya Hiroshi  九州大学, 理学研究院, 名誉教授 (90154658)

Co-Investigator(Kenkyū-buntansha) 迫田 憲治  大阪市立大学, 大学院理学研究科, 准教授 (80346767)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywordsmicrodroplet / ion trap / energy transfer / 微小液滴 / 励起エネルギー移動 / イオントラップ / 界面 / 蛍光プローブ法 / 蛍光プローブ / 界面レーザー分光
Outline of Final Research Achievements

In this research, we developed an experimental apparatus to selectively probe the surface of a single microdroplet levitated in space, and attempted to observe fluorescence excitation energy transfer occurred at the surface. First, a parallel plate ion trap was fabricated to stably levitate a single microdroplet. Microdroplets were generated using an electrospray ionization. Lasing signals from the microdroplet was observed when the droplet in which dye molecules were dissolved were irradiated with a nanosecond pulsed laser. We succeeded in observing the fluorescence excitation energy transfer between the monolayer formed on the surface of the droplet and the dissolved molecule with high sensitivity.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (35 results)

All 2018 2017 2016 2015 2014

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (26 results) (of which Int'l Joint Research: 10 results,  Invited: 7 results) Funded Workshop (1 results)

  • [Journal Article] Real-time observation of the photoionization-induced water rearrangement dynamics in the 5-hydroxyindole-water cluster by time-resolved IR spectroscopy2018

    • Author(s)
      Mitsuhiko Miyazaki, Ayumi Naito, Takamasa Ikeda, Johanna Klyne, Kenji Sakota, Hiroshi Sekiya, Otto Dopfer, Masaaki Fujii
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 20 Issue: 5 Pages: 3079-3091

    • DOI

      10.1039/c7cp06127g

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Elevation of the Energy Threshold for Isomerization of 5-Hydroxyindole-(tert-butyl alcohol)1 Cluster Cations2017

    • Author(s)
      Takamasa Ikeda, Kenji Sakota, Hiroshi Sekiya
    • Journal Title

      J. Phys. Chem. A

      Volume: 121 Issue: 31 Pages: 5809-5816

    • DOI

      10.1021/acs.jpca.7b05434

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two isomers of a Cyanostilbene Derivative in Viscous Solution Observed by Fluorescence Spectroscopy2016

    • Author(s)
      Yuki Fujimoto, Norifumi Yamamoto, Kazuki Furukawa, Hiroshi Sekiya
    • Journal Title

      Chemistry Letters

      Volume: 45 Issue: 6 Pages: 637-639

    • DOI

      10.1246/cl.160143

    • NAID

      130005155029

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Excited-state intramolecular proton transfer and conformational relaxation in 4’-N,N-dimethylamino-3-hydroxyflavone doped in acetonitrile crystals2016

    • Author(s)
      Kazuki Furukawa, Norifumi Yamamoto, Kazuyuki Hino, Hiroshi Sekiya
    • Journal Title

      Physical Chemisty Chemical Physics

      Volume: 18 Issue: 41 Pages: 28564-28575

    • DOI

      10.1039/c6cp04322d

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rearrangements of a Water Molecule in Both Directions between Two Hydrogen-Bonding Sites of 5-Hydroxyindole Cation: Experimental Determination of the Energy Threshold for the Rearrangement2016

    • Author(s)
      T. Ikeda, K. Sakota, H. Sekiya
    • Journal Title

      J. Phys. Chem. A

      Volume: 120 Issue: 11 Pages: 1825-1832

    • DOI

      10.1021/acs.jpca.5b12111

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 水和クラスターにおける励起状態多重プロトン移動2015

    • Author(s)
      関谷 博、迫田憲治
    • Journal Title

      分光研究

      Volume: 64 Pages: 1-15

    • NAID

      40020484675

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature effects on prevalent structures of hydrated Fe+ complexes: Infrared spectroscopy and DFT calculations of Fe+(H2O)n (n=3-8)2014

    • Author(s)
      Kazuhiko Ohahi, Jun Sasaki, Gun Yamamoto, Ken Judai, Nobuyuki Nishi, Hiroshi Sekiya
    • Journal Title

      Journal Chemical Physics

      Volume: 141 Issue: 21

    • DOI

      10.1063/1.4902408

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prologue and Epilogue of Concerted and Stepwise Controversy of Excited-state Double-proton Transfer in a Model DNA Base Pair2014

    • Author(s)
      関谷 博、迫田憲治
    • Journal Title

      Molecular Science

      Volume: 8 Issue: 1 Pages: A0071

    • DOI

      10.3175/molsci.8.A0071

    • NAID

      130004688039

    • ISSN
      1881-8404
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Laser spectroscopy of a single levitated microdroplet ~Lasing and resonance energy transfer~2018

    • Author(s)
      Kenji Sakota
    • Organizer
      International Symposium on "Diversity of Chemical Reaction Dynamics"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 単一微小液滴の顕微分光とバルク溶液中におけるキラルJ会合体の研究2017

    • Author(s)
      迫田憲治
    • Organizer
      第40回溶液化学シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 空間捕捉した単一微小液滴のレーザー顕微分光2017

    • Author(s)
      安冨翔太,佐野元哉,山本駿介,大谷拓也,関谷博,八ツ橋知幸,迫田憲治
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 両親媒性シアニン色素のキラルJ会合体形成における混合溶媒の組成比依存性2017

    • Author(s)
      大谷拓也,三宅弘之,八ツ橋知幸,迫田憲治
    • Organizer
      第40回溶液化学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] External Electric Field Effects on Excited-State Intramolecular Proton Transfer in 4'-N,N-Dimethylamino-3-hydroxyflavone2017

    • Author(s)
      Hiroshi Sekiya
    • Organizer
      XXII Horizons in Hydrogen Bond Research
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Remarkable enhancement of fluorescence quantum yield of the closed-ring form of a diarylethene derivative due to the presence of phenyl rings2017

    • Author(s)
      Hiroshi Sekiya
    • Organizer
      WRHI Syposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Polymorphs-selected fluorescence spectroscopy of salicylideneaniline derivatives: Effects of intermolecular interaction on intramolecular proton transfer and cis-trans isomerization2017

    • Author(s)
      Tomoya Nakagawara, Kiichi Amimoto, Norifumi Yamamoto, Hiroshi Sekiya,
    • Organizer
      KAKENHI International Symposium on “Studying the Function of Soft Molecular Systems”
    • Related Report
      2017 Annual Research Report
  • [Presentation] Dynamics of rearrangement of hydrogen-bonds in monohydrated 5-hydroxyindole cluster cations2016

    • Author(s)
      Kenji Sakota, Takamasa Ikeda, Hiroshi Sekiya
    • Organizer
      Stereodynamics 2016
    • Place of Presentation
      台湾原子分子研究所(台北市)
    • Year and Date
      2016-11-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒドロキシプロピルセルロースの構造転移に対する ホフマイスターアニオンおよびポリエチレングリコールの複合効果2016

    • Author(s)
      米山可凜,関谷博,迫田憲治
    • Organizer
      第10回分子科学討論会
    • Place of Presentation
      神戸ファッションマート(神戸市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] リボヌクレアーゼAの安定性に対する電解質と分子混み合いの複合効果2016

    • Author(s)
      大内仁,関谷博,迫田憲治
    • Organizer
      第10回分子科学討論会
    • Place of Presentation
      神戸ファッションマート(神戸市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] 5-ヒドロキシインドール水和クラスターの時間分解赤外分光 ―イオン化誘起異性化反応ダイナミクスの実時間観測―2016

    • Author(s)
      内藤 あゆみ,宮崎 充彦,迫田 憲治,関谷 博,藤井 正明
    • Organizer
      第10回分子科学討論会
    • Place of Presentation
      神戸ファッションマート(神戸市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] The influence of molecular-weight specific adsorption of polyethylene glycol on two-state transition of myoglobin2016

    • Author(s)
      Shota Yasutomi, Kenni Sakota, Hiroshi Sekiya
    • Organizer
      International Symposium: Recent Progress in Molecular Spectroscopy and Dynamics
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Excited-state dynamics of flexible molecules in solid state2016

    • Author(s)
      Hiroshi Sekiya
    • Organizer
      International Symposium: Recent Progress in Molecular Spectroscopy and Dynamics
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Change in excited-state intramolecular proton transfer potential surfaces of 4’-N,N-dimethyl-3hydroxyflavone doped in acetonitrile crystal accompanying with sold/sold phase transition2016

    • Author(s)
      Kazuki, Furukawa, N. Yamamoto, K. Hino, H. Sekiya
    • Organizer
      International Symposium: Recent Progress in Molecular Spectroscopy and Dynamics
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミオグロビンの2状態転移に対するポリエチレングリコールの吸着の効果とその分子量特異性2016

    • Author(s)
      安冨翔太,迫田憲治,関谷博
    • Organizer
      日本化学会第96春季年会(2016)
    • Place of Presentation
      同志社大学 京田辺キャンパス
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 混み合い環境におけるポリ(N-イソプロピルアクリルアミド)の構造転移に対するホフマイスター効果2016

    • Author(s)
      米山可凜,迫田憲治,関谷博
    • Organizer
      日本化学会第96春季年会(2016)
    • Place of Presentation
      同志社大学 京田辺キャンパス
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] The influence of molecular-weight specific adsorption of polyethylene glycol on two-state transition of myoglobin2015

    • Author(s)
      Shota Yasutomi, Kenji Sakota, Hiroshi Sekiya
    • Organizer
      Pacifichem2015
    • Place of Presentation
      米国ハワイ州ホノルル市
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rearrangement of hydrogen bond network in monohydrated 5-hydroxyindole cluster cations2015

    • Author(s)
      Takamasa Ikeda, Kenji Sakota, Hiroshi Sekiya
    • Organizer
      Pacifichem2015
    • Place of Presentation
      米国ハワイ州ホノルル市
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] IR spectroscopy of 5-hydroxyindole-methanol in the gas phase: Rearrangement of H-bond2015

    • Author(s)
      Karin Yoneyama, Kenji Sakota, Hiroshi Sekiya
    • Organizer
      Pacifichem2015
    • Place of Presentation
      米国ハワイ州ホノルル市
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミオグロビンの2状態転移に対するポリエチレングリコールの分子量特異的吸着とその効果2015

    • Author(s)
      安冨翔太,迫田憲治,関谷博
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 5-ヒドロキシインドール水和クラスターカチオンにおける水和構造組み換えの観測2015

    • Author(s)
      池田貴将,迫田憲治,関谷博
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] ポリ(N-イソプロピルアクリルアミド)の相転移に対するHofmeisterイオンおよび巨大分子混み合いの複合効果2015

    • Author(s)
      米山可凜,迫田憲治,関谷博
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 超高感度バイオセンシングを目指した単一微小液滴顕微分光装置の開発2015

    • Author(s)
      迫田憲治,関谷博
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      東京工業大学
    • Related Report
      2015 Annual Research Report
  • [Presentation] ポリ(N-イソプロピルアクリルアミド)の構造転移に対する 巨大分子の混み合いと塩添加の複合効果2014

    • Author(s)
      田端大樹,迫田憲治,関谷博
    • Organizer
      第37回溶液化学シンポジウム
    • Place of Presentation
      アバンセ(佐賀市)
    • Year and Date
      2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 温度応答性高分子へのアニオン吸着挙動に対する共存巨大分子の混み合い効果:ランダムコイル-グルビュール転移への影響2014

    • Author(s)
      迫田憲治,田端大樹,関谷博
    • Organizer
      第8回分子科学討論会
    • Place of Presentation
      広島大学
    • Year and Date
      2014-09-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Excited-state multiple-proton transfer in hydrated 7-azaindole clusters in the gas phase2014

    • Author(s)
      Hiroshi Sekiya, Kenji Sakota, Gustavo Pino, Claude Dedonder, Christophe Jouvet
    • Organizer
      248th ACS National Meeting
    • Place of Presentation
      San Francisco Convention Center
    • Year and Date
      2014-08-10 – 2014-08-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Funded Workshop] Recent Progress in Molecular Spectroscopy and Dynamics2016

    • Place of Presentation
      Kyushu University Nishijin Plaza
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2019-03-29  

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