Development of surface selective high-sensitivity fluorescent probe apparatus for microdroplets and its application to biomolecules
| Project/Area Number |
26288010
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Sekiya Hiroshi 九州大学, 理学研究院, 名誉教授 (90154658)
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| Co-Investigator(Kenkyū-buntansha) |
迫田 憲治 大阪市立大学, 大学院理学研究科, 准教授 (80346767)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Keywords | microdroplet / ion trap / energy transfer / 微小液滴 / 励起エネルギー移動 / イオントラップ / 界面 / 蛍光プローブ法 / 蛍光プローブ / 界面レーザー分光 |
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| Outline of Final Research Achievements |
In this research, we developed an experimental apparatus to selectively probe the surface of a single microdroplet levitated in space, and attempted to observe fluorescence excitation energy transfer occurred at the surface. First, a parallel plate ion trap was fabricated to stably levitate a single microdroplet. Microdroplets were generated using an electrospray ionization. Lasing signals from the microdroplet was observed when the droplet in which dye molecules were dissolved were irradiated with a nanosecond pulsed laser. We succeeded in observing the fluorescence excitation energy transfer between the monolayer formed on the surface of the droplet and the dissolved molecule with high sensitivity.
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Report
(5 results)
Research Products
(35 results)
