Development of supramolecular construction techniques of proteins and their functionalization using domain swapping
| Project/Area Number |
26288080
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
HIROTA Shun 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (90283457)
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| Co-Investigator(Renkei-kenkyūsha) |
HIGUCHI Yoshiki 兵庫県立大学, 大学院生命理学研究科, 教授 (90183574)
NAGAO Satoshi 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (30452535)
YAMANAKA Masaru 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (60632825)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | タンパク質 / 生体分子 / ナノバイオ / 超分子化学 / 多量体 / 立体構造 / ドメインスワッピング / 蛋白質 / 超分子 |
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| Outline of Final Research Achievements |
A certain amount of molten globule state oligomers of horse cyt c converted to domain-swapped oligomers by folding from the acid molten globule state to neutral pH state under high protein concentrations. Domain-swapped dimer stability of horse cyt c was less affected by the Met80 coordination to the heme. Two domain-swapped structures of thermostable cyt c552 were detected for its hinge loop mutant. Domain swapping was shown to also occur for thermostable cyt c552 when expressed in E. coli cells. A tetramer of cyt c′ was constructed with one domain-swapped dimer and two monomers. An artificial myoglobin heterodimer with two different heme active sites was constructed based on domain swapping.
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Report
(4 results)
Research Products
(71 results)
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[Presentation] Domain Swapping in c-Type Cytochromes and Myoglobin2016
Author(s)
Shun Hirota, Satoshi Nagao, Partha Pratim Parui, Megha Subhash Deshpande, Chunguang Ren, Yugo Hayashi, Takaaki Miyamoto, Yin-Wu Lin, and Yoshiki Higuchi
Organizer
30th Anniversary Symposium of the Protein Society
Place of Presentation
Baltimore, USA
Year and Date
2016-07-16
Related Report
Int'l Joint Research
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