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Development of supramolecular construction techniques of proteins and their functionalization using domain swapping

Research Project

Project/Area Number 26288080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionNara Institute of Science and Technology

Principal Investigator

HIROTA Shun  奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (90283457)

Co-Investigator(Renkei-kenkyūsha) HIGUCHI Yoshiki  兵庫県立大学, 大学院生命理学研究科, 教授 (90183574)
NAGAO Satoshi  奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (30452535)
YAMANAKA Masaru  奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (60632825)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywordsタンパク質 / 生体分子 / ナノバイオ / 超分子化学 / 多量体 / 立体構造 / ドメインスワッピング / 蛋白質 / 超分子
Outline of Final Research Achievements

A certain amount of molten globule state oligomers of horse cyt c converted to domain-swapped oligomers by folding from the acid molten globule state to neutral pH state under high protein concentrations. Domain-swapped dimer stability of horse cyt c was less affected by the Met80 coordination to the heme. Two domain-swapped structures of thermostable cyt c552 were detected for its hinge loop mutant. Domain swapping was shown to also occur for thermostable cyt c552 when expressed in E. coli cells. A tetramer of cyt c′ was constructed with one domain-swapped dimer and two monomers. An artificial myoglobin heterodimer with two different heme active sites was constructed based on domain swapping.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(71 results)

All 2017 2016 2015 2014

All Journal Article (16 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 16 results,  Acknowledgement Compliant: 16 results,  Open Access: 4 results) Presentation (55 results) (of which Int'l Joint Research: 14 results,  Invited: 13 results)

  • [Journal Article] Formation and Carbon Monoxide-Dependent Dissociation of Allochromatium vinosum Cytochrome c' Oligomers Using Domain-Swapped Dimers2017

    • Author(s)
      M. Yamanaka, M. Hoshizumi, S. Nagao, R. Nakayama, N. Shibata, Y. Higuchi, and S. Hirota
    • Journal Title

      Protein Science

      Volume: 26 Pages: 464-474

    • DOI

      10.1002/pro.3090

    • NAID

      120006334944

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Improved Stopped-Flow Time-Resolved Resonance Raman Spectroscopy Device for Studying Enzymatic Reactions2017

    • Author(s)
      S. Yanagisawa, M. S. Deshpande, S. Hirota, T. Nakagawa, and T. Ogura
    • Journal Title

      Journal of RAMAN SPECTROSCOPY

      Volume: 48 Pages: 680-685

    • DOI

      10.1002/jrs.5100

    • NAID

      120006334945

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effect of Methionine80 Heme Coordination on Domain Swapping of Cytochrome c2017

    • Author(s)
      S. Hirota, N. Yamashiro, Z. Wang, and S. Nagao
    • Journal Title

      J. Biol. Inorg. Chem.

      Volume: 22 Pages: 705-712

    • DOI

      10.1007/s00775-017-1446-3

    • NAID

      120006334946

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A Single Spherical Assembly of Protein Amyloid Fibrils Formed by Laser Trapping2017

    • Author(s)
      K. Yuyama, M. Ueda, S. Nagao, S. Hirota, T. Sugiyama, and H. Masuhara
    • Journal Title

      Angew. Chem. Int. Ed. (Hot Paper)

      Volume: 56 Pages: 6739-6743

    • DOI

      10.1002/anie.201702352

    • NAID

      120006473600

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Characterization of Cytochrome c Membrane-Binding Site Using Cardiolipin-Containing Bicelles with NMR2016

    • Author(s)
      H. Kobayashi, S. Nagao, and S. Hirota
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 55 Pages: 14019-14022

    • DOI

      10.1002/anie.201607419

    • NAID

      120005850423

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Domain swapping oligomerization of thermostable c-type cytochrome in E. coli cells2016

    • Author(s)
      Yugo Hayashi, Masaru Yamanaka, Satoshi Nagao, Hirofumi Komori, Yoshiki Higuchi, Shun Hirota
    • Journal Title

      Scientific Reports

      Volume: 6 Pages: 19334-19334

    • DOI

      10.1038/srep19334

    • NAID

      120006334942

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Domain-swapped Cytochrome cb562 Dimer and Its Nanocage Encapsulating a Zn-SO4 Cluster in the Internal Cavity2015

    • Author(s)
      Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
    • Journal Title

      Chem. Sci.

      Volume: 6 Pages: 7336-7342

    • DOI

      10.1039/c5sc02428e

    • NAID

      120006334940

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Oligomerization Enhancement and Two Domain Swapping Mode Detection for Thermostable Cytochrome c-552 via the Elongation of the Major Hinge Loop2015

    • Author(s)
      C. Ren, S. Nagao, M. Yamanaka, H. Komori, Y. Shomura, Y. Higuchi and S. Hirota
    • Journal Title

      Molecular BioSystems

      Volume: 11(12) Pages: 218-221

    • DOI

      10.1039/c5mb00545k

    • NAID

      120006334941

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rational design of heterodimeric protein using domain swapping for myoglobin2015

    • Author(s)
      Ying-Wu Lin, Satoshi Nagao, Mohan Zhang, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 54 Pages: 511-515

    • DOI

      10.1002/anie.201409267

    • NAID

      120005716859

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Change in Structure and Ligand Binding Properties of Hyperstable Cytochrome c555 from Aquifex aeolicus by Domain Swapping2015

    • Author(s)
      M. Yamanaka, S. Nagao, H. Komori, Y. Higuchi and S. Hirota
    • Journal Title

      Protein Science

      Volume: 24 Pages: 366-375

    • DOI

      10.1002/pro.2627

    • NAID

      120005716876

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Carbon monoxide binding properties of domain-swapped dimeric myoglobin2015

    • Author(s)
      Satoshi Nagao, Haruto Ishikawa, Takuya Yamada, Yasuhisa Mizutani, Shun Hirota
    • Journal Title

      J. Biol. Inorg. Chem.

      Volume: 20 Pages: 523-530

    • DOI

      10.1007/s00775-014-1236-0

    • NAID

      120005716870

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Domain-swapped dimer of Pseudomonas aeruginosa cytochrome c551: Structural insights into domain swapping of cytochrome c family proteins2015

    • Author(s)
      Satoshi Nagao, Mariko Ueda, Hisao Osuka, Hirofumi Komori, Hironari Kamikubo, Mikio Kataoka, Yoshiki Higuchi, Shun Hirota
    • Journal Title

      PLOS ONE

      Volume: 10

    • DOI

      10.1371/journal.pone.0123653

    • NAID

      120005716860

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Formation of Domain-Swapped Oligomer of Cytochrome c from Its Molten Globule State Oligomer2014

    • Author(s)
      Megha Deshpande, Partha Parui, Hironari Kamikubo, Masaru Yamanaka, Satoshi Nagao, Hirofumi Komori, Mikio Kataoka, Yoshiki Higuchi, Shun Hirota
    • Journal Title

      Biochemistry

      Volume: 53(28) Pages: 4696-4703

    • DOI

      10.1021/bi500497s

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Self-Oxidation of Cytochrome c at Methionine80 with Molecular Oxygen Induced by Cleavage of the Met-Heme Iron Bond2014

    • Author(s)
      Z. Wang, Y. Ando, A. D. Nugraheni, C. Ren, S. Nagao, S. Hirota
    • Journal Title

      Molecular BioSystems

      Volume: 10 Pages: 3130-3137

    • DOI

      10.1039/c4mb00285g

    • NAID

      120005716873

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] DNA Cleavage by Oxymyoglobin and Cysteine-Introduced Metmyoglobin2014

    • Author(s)
      M. S. Deshpande, S. Junedi, H. Prakash, S. Nagao, M. Yamanaka, S. Hirota
    • Journal Title

      Chemical Communications

      Volume: 50 Pages: 15034-15036

    • DOI

      10.1039/c4cc06617k

    • NAID

      120005716862

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Control of Transition between Ni-C and Ni-SIa States in Catalytic Cycle of [NiFe] Hydrogenase by Redox State of its Proximal Fe-S Cluster2014

    • Author(s)
      H. Tai, K. Nishikawa, M. Suzuki, Y. Higuchi, and S. Hirota
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 53 Pages: 13817-13820

    • DOI

      10.1002/anie.201408552

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Design of Domain-swapped Myoglobin Dimer2017

    • Author(s)
      Satoshi Nagao, Ayaka Suda, Hisashi Kobayashi, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota
    • Organizer
      97th Chemical Society of Japan Annual Meeting
    • Place of Presentation
      慶応義塾大学(神奈川県横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Rhodopseudomonas palustris由来シトクロムc′の多量化2017

    • Author(s)
      久保田真史, 宮本昂明、廣田俊
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応義塾大学(神奈川県横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Laser Trapping Dynamics of Amyloid Fibril Formation of Cytochrome c Monomer and Dimers2017

    • Author(s)
      Chieh-Ju Chang, Yugo Hayashi, Shun Hirota, Ken-ichi Yuyama, Teruki Sugiyama, and Hirotashi Masuhara
    • Organizer
      97th Chemical Society of Japan Annual Meeting
    • Place of Presentation
      慶応義塾大学(神奈川県横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effect of Rare Codons on Hydrogenobacter thermophiles cyt c552 Oligomerization in E. coli Cells2017

    • Author(s)
      Hongxu Yang, Masaru Yamanaka, Satoshi Nagao, and Shun Hirota
    • Organizer
      97th Chemical Society of Japan Annual Meeting
    • Place of Presentation
      慶応義塾大学(神奈川県横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Formation of Cytochrome c Oligomers Using Domain-Swapped Dimers and Carbon Monoxide-Dependent Control of Oligomer Association/Dissociation2017

    • Author(s)
      Masaru Yamanaka, Ryoko Nakayama, Satoshi Nagao, Makoto Hoshizumi, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota
    • Organizer
      97th Chemical Society of Japan Annual Meeting
    • Place of Presentation
      慶応義塾大学(神奈川県横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of the Cytochrome c Membrane-binding Site Using Solution NMR2017

    • Author(s)
      Hisashi Kobayashi, Satoshi Nagao, and Shun Hirota
    • Organizer
      97th Chemical Society of Japan Annual Meeting
    • Place of Presentation
      慶応義塾大学(神奈川県横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Protein Nanostructures Constructed by Fusing Two Oligomerization Domains with a 3-Helix Bundle Linker2017

    • Author(s)
      Takaaki Miyamoto and Shun Hirota
    • Organizer
      97th Chemical Society of Japan Annual Meeting
    • Place of Presentation
      慶応義塾大学(神奈川県横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Construction of a Triangle-shaped Structure with a Unit Protein Based on Cytochrome c5552017

    • Author(s)
      Akiya Oda, Ikki Ueda, Masaru Yamanaka, Satoshi Nagao, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota
    • Organizer
      97th Chemical Society of Japan Annual Meeting
    • Place of Presentation
      慶応義塾大学(神奈川県横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Cytochrome c: Domain Swapping, Self-Modification, and Molecular Recognition2017

    • Author(s)
      Shun Hirota, Satoshi Nagao, Masaru Yamanaka, and Yoshiki Higuchi
    • Organizer
      5th Symposium on Advanced Biological Inorganic Chemistry
    • Place of Presentation
      Kolkata, India
    • Year and Date
      2017-01-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Construction of Heme Protein Supramolecules by Domain Swapping2016

    • Author(s)
      Shun Hirota, Satoshi Nagao, Masaru Yamanaka, and Yoshiki Higuchi
    • Organizer
      5th Symposium on Advanced Biological Inorganic Chemistry
    • Place of Presentation
      Auckland, New Zealand
    • Year and Date
      2016-12-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Solution NMR Characterization of the Interaction between Cyt c and Cardiolipin-incorporated Bicelles2016

    • Author(s)
      Hisashi Kobayashi, Satoshi Nagao, and Shun Hirota
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Analysis of Environment Dependent Dynamics of Cytochrome c on a Lipid Membrane by 2D Fluorescence Lifetime Correlation Spectroscopy2016

    • Author(s)
      Miyuki Sakaguchi,Masaru Yamanaka,Shun Hirota,Kunihiko Ishii,and Tahei Tahara
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Formation of Myoglobin Dimers by Domain Swapping2016

    • Author(s)
      Satoshi Nagao, Ayaka Suda, Hisashi Kobayashi, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Structure and Function of Heme Protein Oligomers Constructed by Domain Swapping2016

    • Author(s)
      Shun Hirota
    • Organizer
      Japan-Korea-Taiwan Bioinorganic Chemistry Symposium
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-09-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Application of Supramolecular Science for Drug Design2016

    • Author(s)
      Shun Hirota
    • Organizer
      The Symposium of Annual Scientific Meeting 2016 of Indonesian Pharmacist Association
    • Place of Presentation
      Jogjakarta, Indonesia
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 二次元蛍光寿命相関分光法によるウマシトクロムcのフォールディング機構の研究2016

    • Author(s)
      坂口美幸、山中優、廣田俊、石井邦彦、田原太平
    • Organizer
      第10回分子科学討論会
    • Place of Presentation
      神戸ファッションマート(兵庫県神戸市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] 過酸との反応によるシトクロムcのMet80の酸化修飾2016

    • Author(s)
      松本順文、REN Chunguang、山中優、長尾聡、廣田俊
    • Organizer
      第10回バイオ関連化学シンポジウム
    • Place of Presentation
      石川県立音楽堂・もてなしドーム地下イベント広場(石川県金沢市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] ミオグロビンのループ領域アミノ酸変異によるドメインスワップ二量体のデザイン2016

    • Author(s)
      須田綾香、小林紀、長尾聡、柴田直樹、樋口芳樹、廣田俊
    • Organizer
      第10回バイオ関連化学シンポジウム
    • Place of Presentation
      石川県立音楽堂・もてなしドーム地下イベント広場(石川県金沢市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] Domain Swapping in c-Type Cytochromes and Myoglobin2016

    • Author(s)
      Shun Hirota, Satoshi Nagao, Partha Pratim Parui, Megha Subhash Deshpande, Chunguang Ren, Yugo Hayashi, Takaaki Miyamoto, Yin-Wu Lin, and Yoshiki Higuchi
    • Organizer
      30th Anniversary Symposium of the Protein Society
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2016-07-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of Zn-SO4 Cluster-encapsulating Protein Nanocage by Domain Swapping2016

    • Author(s)
      Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, and Shun Hirota
    • Organizer
      30th Anniversary Symposium of the Protein Society
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2016-07-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of the Interaction between Cyt c and Cardiolipin-incorporated by Solution NMR2016

    • Author(s)
      Hisashi Kobayashi, Satoshi Nagao, and Shun Hirota
    • Organizer
      30th Anniversary Symposium of the Protein Society
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2016-07-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of Zn-Domain Swapping of c-Type Cytochromes and Myoglobin2016

    • Author(s)
      Shun Hirota
    • Organizer
      Ninth International Conference on Porphyrins and Phthalocyanines
    • Place of Presentation
      Nanjing, China
    • Year and Date
      2016-07-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] バイセルを利用したシトクロムcの脂質膜結合部位のNMR研究2016

    • Author(s)
      小林紀、長尾聡、廣田俊
    • Organizer
      第43回生体分子科学討論会
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Year and Date
      2016-06-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 溶液NMRによるシトクロムcとカルジオリピン含有バイセルの相互作用解析2016

    • Author(s)
      小林紀、長尾聡、廣田俊
    • Organizer
      第26回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2016-06-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 四量体シトクロムc’の作製とCO分子結合・解離によるサイズ制御2016

    • Author(s)
      山中優、星住誠人、長尾聡、廣田俊
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] ドメインスワップしたシトクロムcb562二量体が形成するZn-SO4クラスター内包タンパク質ナノケージの結晶構造2016

    • Author(s)
      宮本昂明、長尾聡、庄村康人、樋口芳樹、廣田俊
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of the Interaction of Cytochrome c with Cardiolipin-incorporated Lipid Bicelle2016

    • Author(s)
      Hisashi Kobayashi, Satoshi Nagao, Shun Hirota
    • Organizer
      96th Spring Conference of the Chemical Society of Japan
    • Place of Presentation
      Doshisha University (Kyotanabe, Kyoto)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Domain-swapped Cytochrome cb562 Dimer and Its Nanocage Encapsulating a Zn-SO4 Cluster in the Internal Cavity2016

    • Author(s)
      Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
    • Organizer
      96th Spring Conference of the Chemical Society of Japan
    • Place of Presentation
      Doshisha University (Kyotanabe, Kyoto)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] ドメインスワップ構造に基づいた安定なミオグロビン二量体のデザイン2016

    • Author(s)
      須田綾香、小林紀、長尾聡、廣田俊
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京田辺市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Construction of Cytochrome c' Tetramer and Its Size Control by CO Binding2016

    • Author(s)
      山中優、星住誠人、廣田俊
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京田辺市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Construction of Heme Protein Oligomers by Domain Swapping2015

    • Author(s)
      Shun Hirota
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Hilton Hawaiian Village (Honolulu, USA)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 好熱菌シトクロムc552のドメインスワップ多量体形成2015

    • Author(s)
      林有吾、山中優、長尾聡、小森博文、樋口芳樹、廣田俊
    • Organizer
      日本生物高分子学会2015年度大会
    • Place of Presentation
      香川大学(香川県高松市)
    • Year and Date
      2015-09-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] ドメインスワップ構造に基づくミオグロビン二量体のデザイン2015

    • Author(s)
      長尾聡、Ying-Wu Lin、Mohan Zhang、庄村康人、樋口芳樹、廣田俊
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本大学(熊本県熊本市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] シトクロムcとカルジオリピン含有脂質膜の相互作用解析2015

    • Author(s)
      小林紀、長尾聡、廣田俊
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本大学(熊本県熊本市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] ドメインスワッピングとは?:タンパク質の超分子化2015

    • Author(s)
      廣田俊
    • Organizer
      第28回生物無機化学夏季セミナー
    • Place of Presentation
      山喜旅館(静岡県伊東市)
    • Year and Date
      2015-08-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Spatio-Temporal Formation of Amyloid Aggregates by Laser Trapping of Domain-swapped Dimeric Cytochrome c2015

    • Author(s)
      Ken-ichi Yuyama, Mariko Ueda, Satoshi Nagao, Shun Hirota, Teruki Suigiyama, Hiroshi Masuhara
    • Organizer
      Optical Trapping and Optical Micromanipulation XII, SPIE NanoScience + Engineering Conference
    • Place of Presentation
      San Diego Convention Center (San Diego, USA)
    • Year and Date
      2015-08-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Formation of Cytochrome cb562 Oligomers by Domain Swapping2015

    • Author(s)
      Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
    • Organizer
      29th Annual Symposium of The Protein Society
    • Place of Presentation
      Palau de Congressos de Barcelona (Barcelona, Spain)
    • Year and Date
      2015-07-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of Dimeric Heme Proteins by Domain Swapping2015

    • Author(s)
      Shun Hirota
    • Organizer
      17th International Conference on Biological Inorganic Chemistry (ICBIC17)
    • Place of Presentation
      China National Convention Center (Beijing, China)
    • Year and Date
      2015-07-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cytochrome c and Myoglobin Oligomers Formed by Domain Swapping2015

    • Author(s)
      Shun Hirota
    • Organizer
      International Symposium on Metal Complexes (ISMEC2015)
    • Place of Presentation
      Hotel Plaza (Wroclaw, Poland)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Change in Structure and Ligand Binding Properties of Cytochrome c555 from Hyperthermophile by Domain Swapping2015

    • Author(s)
      山中優、長尾聡、小森博文、樋口芳樹、廣田俊
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] NMRによるシトクロムcとカルジオリピンの相互作用解析2015

    • Author(s)
      小林紀、長尾聡、廣田俊
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Domain Swapping of c-Type Cytochromes and Myoglobin2015

    • Author(s)
      Shun Hirota, Satoshi Nagao, Masaru Yamanaka, Hirofumi Komori, Yasuhito Shomura, Yoshiki Higuchi
    • Organizer
      "Metals in Biology" in Wako
    • Place of Presentation
      Riken Wako Campus (Wako, Saitama)
    • Year and Date
      2015-06-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ドメインスワップしたシトクロムcb562の構造と性質2015

    • Author(s)
      宮本昂明、栗林麻衣、長尾聡、庄村康人、樋口芳樹、廣田俊
    • Organizer
      第25回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      長崎大学(長崎県長崎市)
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] ドメインスワッピングを利用したシトクロムcおよびミオグロビンの多量体形成2015

    • Author(s)
      長尾聡、山中優、Ying-Wu Lin、Megha Subhash Deshpande、小森博文、庄村康人、樋口芳樹、廣田俊
    • Organizer
      第25回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      長崎大学(長崎県長崎市)
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Crystal structure and function of a domain-swapped cytochrome cb562 dimer2015

    • Author(s)
      Takaaki Miyamoto, Mai Kuribayashi, Satoshi Nagao, Yasuhito Shomura, Yoshiki Higuchi, Shun Hirota
    • Organizer
      95th Spring Conference of the Chemical Society of Japan
    • Place of Presentation
      Nihon University (Funabashi)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Construction of high-order domain-swapped oligomers of Hydrogenobacter thermophilus cytochrome c552 by hinge loop mutation2015

    • Author(s)
      Chunguang Ren, Masaru Yamanaka, Satoshi Nagao, Shun Hirota
    • Organizer
      95th Spring Conference of the Chemical Society of Japan
    • Place of Presentation
      Nihon University (Funabashi)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Design and properties of domain-swapped dimeric myoglobin2015

    • Author(s)
      Satoshi Nagao, Ying-Wu Lin, Haruto Ishikawa, Takuya Yamada, Mohan Zhang, Yasuhito Shomura, Yasuhisa Mizutani, Yoshiki Higuchi, Shun Hirota
    • Organizer
      95th Spring Conference of the Chemical Society of Japan
    • Place of Presentation
      Nihon University (Funabashi)
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Formation of domain-swapped oligomer of Hydrogenobacter thermophiles cytochrome c552 in an expression system2015

    • Author(s)
      Yugo Hayashi, Satoshi Nagao, Masaru Yamanaka, Hirofumi Komori, Yoshiki Higuchi, Shun Hirota
    • Organizer
      95th Spring Conference of the Chemical Society of Japan
    • Place of Presentation
      Nihon University (Funabashi)
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 緑膿菌由来シトクロムc551と好熱性水素細菌由来シトクロムc552のキメラタンパク質を用いたヘテロダイマーの構築2015

    • Author(s)
      中西司、山中優、廣田俊
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      日本大学(船橋)
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヘムタンパク質の多量化:タンパク質変性と機能化(2)2015

    • Author(s)
      廣田俊
    • Organizer
      分子研研究会「生物無機化学の最先端と今後の展望:金属と生体分子の相互作用機序解明とモデル化および応用への展開」
    • Place of Presentation
      分子科学研究所(岡崎市)
    • Year and Date
      2015-01-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Design and construction of protein supramolecules for anticancer agent and DDS2014

    • Author(s)
      Shun Hirota
    • Organizer
      Medan International Conference on Advanced Pharmaceutical Sciences (MICAPS 2014)
    • Place of Presentation
      Medan (Indonesia)
    • Year and Date
      2014-11-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] シトクロム cb562の多量化と二量体構造2014

    • Author(s)
      宮本昂明、栗林麻衣、長尾聡、庄村康人、樋口芳樹、廣田俊
    • Organizer
      第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 生理的条件下でのシトクロムc多量体形成2014

    • Author(s)
      林有吾、中山璃子、長尾聡、山中優、小森博文、樋口芳樹、廣田俊
    • Organizer
      第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] ドメインスワッピングによるヘムタンパク質の会合2014

    • Author(s)
      廣田俊
    • Organizer
      第24回金属の関与する生体関連反応シンポジウム
    • Place of Presentation
      京都薬科大学(京都市)
    • Year and Date
      2014-06-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ドメインスワッピングを利用したヘムタンパク質の超分子化2014

    • Author(s)
      廣田俊
    • Organizer
      第41回生体分子科学討論会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2014-06-07
    • Related Report
      2014 Annual Research Report
    • Invited

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Published: 2014-04-04   Modified: 2018-03-22  

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