Structural basis for triplex nucleic acid recognition of triplex DNA binding proteins and application of triplex DNA binding proteins to artificial regulation of target gene expression
| Project/Area Number |
26288084
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NOMURA Yuusuke 国立医薬品食品衛生研究所, 医療機器部, 任期付研究員 (10609308)
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| Research Collaborator |
KIUCHI Kazuki
SUGIYAMA Kohta
KISHI Ryotaro
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | 3本鎖核酸結合蛋白質 / 3本鎖核酸 / 3本鎖核酸認識 / 超遠心分析 / X線小角散乱 / 等温滴定型熱量計 / 3本鎖核酸形成促進 / 転写制御 / 円偏光二色性スペクトル / 構造的基盤 / 人工的遺伝子発現制御 / in vitro転写系 |
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| Outline of Final Research Achievements |
Triplex nucleic acid is formed when a single-stranded oligonucleotide binds to the target duplex DNA. Triplex nucleic acid formation is involved in the regulation of the downstream target gene expression. Triplex DNA binding proteins may be possibly involved in the regulation of the downstream target gene expression by binding to the triplex nucleic acid. In this study, we revealed the structural basis for triplex nucleic acid recognition of triplex DNA binding proteins. Also, we found that triplex DNA binding proteins promoted triplex nucleic acid formation, although triplex nucleic acid formation was usually unstable under physiological condition. Furthermore, we found that triplex nucleic acid formation repressed downstream target gene transcription to yield shorter transcript RNA.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] The Crystal Structure of a 2',4'-BNANC[N-Me]-modified Antisense Gapmer in Complex with the Target RNA2016
Author(s)
Kondo, J., Nomura, Y., Kitahara, Y., Obika, S., Torigoe, H.
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Journal Title
Chem. Commun.
Volume: 52
Pages: 2354-2357
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Structures, physicochemical properties, and applications of T-HgII-T, C-AgI-C, and other metallo-base-pairs.2015
Author(s)
Yoshiyuki Tanaka, Jiro Kondo, Vladimir Sychrovsky, Jakub Sebera, Takenori Dairaku, Hisao Saneyoshi, Hidehito Urata, Hidetaka Torigoe and Akira Ono
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Journal Title
Chem. Comm.
Volume: 51
Pages: 17313-17360
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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