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Structural basis for triplex nucleic acid recognition of triplex DNA binding proteins and application of triplex DNA binding proteins to artificial regulation of target gene expression

Research Project

Project/Area Number 26288084
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionTokyo University of Science

Principal Investigator

Torigoe Hidetaka  東京理科大学, 理学部, 教授 (80227678)

Co-Investigator(Renkei-kenkyūsha) NOMURA Yuusuke  国立医薬品食品衛生研究所, 医療機器部, 任期付研究員 (10609308)
Research Collaborator KIUCHI Kazuki  
SUGIYAMA Kohta  
KISHI Ryotaro  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywords3本鎖核酸結合蛋白質 / 3本鎖核酸 / 3本鎖核酸認識 / 超遠心分析 / X線小角散乱 / 等温滴定型熱量計 / 3本鎖核酸形成促進 / 転写制御 / 円偏光二色性スペクトル / 構造的基盤 / 人工的遺伝子発現制御 / in vitro転写系
Outline of Final Research Achievements

Triplex nucleic acid is formed when a single-stranded oligonucleotide binds to the target duplex DNA. Triplex nucleic acid formation is involved in the regulation of the downstream target gene expression. Triplex DNA binding proteins may be possibly involved in the regulation of the downstream target gene expression by binding to the triplex nucleic acid. In this study, we revealed the structural basis for triplex nucleic acid recognition of triplex DNA binding proteins. Also, we found that triplex DNA binding proteins promoted triplex nucleic acid formation, although triplex nucleic acid formation was usually unstable under physiological condition. Furthermore, we found that triplex nucleic acid formation repressed downstream target gene transcription to yield shorter transcript RNA.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (18 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] The Crystal Structure of a 2',4'-BNANC[N-Me]-modified Antisense Gapmer in Complex with the Target RNA2016

    • Author(s)
      Kondo, J., Nomura, Y., Kitahara, Y., Obika, S., Torigoe, H.
    • Journal Title

      Chem. Commun.

      Volume: 52 Issue: 11 Pages: 2354-2357

    • DOI

      10.1039/c5cc08300a

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structures, physicochemical properties, and applications of T-HgII-T, C-AgI-C, and other metallo-base-pairs.2015

    • Author(s)
      Yoshiyuki Tanaka, Jiro Kondo, Vladimir Sychrovsky, Jakub Sebera, Takenori Dairaku, Hisao Saneyoshi, Hidehito Urata, Hidetaka Torigoe and Akira Ono
    • Journal Title

      Chem. Commun.

      Volume: 51 Issue: 98 Pages: 17313-17360

    • DOI

      10.1039/c5cc02693h

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 3本鎖DNA結合蛋白質の3本鎖DNA認識機構と遺伝子発現制御機構2016

    • Author(s)
      木内一樹、杉山航太、岸遼太郎、佐藤憲大、片山拓馬、鳥越秀峰
    • Organizer
      第39回日本分子生物学会年会シンポジウム「非B型DNA:ゲノム配列に隠された非標準構造の未踏機能の解明に向けて」
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 3本鎖DNA結合蛋白質の3本鎖DNA認識機構と遺伝子発現制御機構2016

    • Author(s)
      木内一樹、杉山航太、岸遼太郎、佐藤憲大、片山拓馬、鳥越秀峰
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular Mechanism of the Triplex DNA-binding Protein to Recognize Triplex DNA2016

    • Author(s)
      Kiuchi, K., Sugiyama, K., Kishi, R., Unzai, S., Torigoe, H.
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effect of Triplex DNA Formation and Triplex DNA Binding Proteins on Transcriptional Activity of T7 RNA Polymerase2016

    • Author(s)
      Sugiyama, K., Kiuchi, K., Torigoe, H.
    • Organizer
      The 43rd International Symposium on Nucleic Acids Chemistry 2016(国際学会)
    • Place of Presentation
      熊本大学黒髪南キャンパス(熊本県熊本市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 3本鎖DNA形成および3本鎖DNA結合蛋白質がT7 RNAポリメラーゼの転写活性に及ぼす影響2016

    • Author(s)
      杉山航太、木内一樹、鳥越秀峰
    • Organizer
      日本化学会第96春期年会
    • Place of Presentation
      同志社大学京田辺キャンパス(京都府京田辺市)
    • Year and Date
      2016-03-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] 人工的遺伝子発現制御法であるアンチジーン法の実用化を指向した3本鎖DNA結合蛋白質STM1による3本鎖DNA形成促進2015

    • Author(s)
      木内一樹、佐々木澄美、杉山航太、間瀬貴久江、佐藤憲大、片山拓馬、鳥越秀峰
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] 3本鎖DNA結合蛋白質の3本鎖DNA認識機構2015

    • Author(s)
      木内一樹、間瀬貴久江、佐藤憲大、片山拓馬、鳥越秀峰
    • Organizer
      第5回日本化学会化学フェスタ2015
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-10-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 3本鎖核酸形成用単鎖核酸の化学修飾が3本鎖核酸形成に及ぼす効果2015

    • Author(s)
      鳥越秀峰、小比賀聡、佐々木澄美
    • Organizer
      第51回熱測定討論会
    • Place of Presentation
      東京電機大学埼玉鳩山キャンパス(埼玉県鳩山市)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular Mechanism of Triplex DNA-binding Proteins to Recognize Triplex DNA2015

    • Author(s)
      Kiuchi, K, Mase, K., Sato, N., Katayama, T., Torigoe, H.
    • Organizer
      The 42nd International Symposium on Nucleic Acids Chemistry 2015
    • Place of Presentation
      あいめっせホール(兵庫県姫路市)
    • Year and Date
      2015-09-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular Mechanism of Triplex DNA-Binding Proteins to Recognize Triplex DNA2015

    • Author(s)
      Torigoe, H., Kiuchi, K., Mase, K., Sato, N., Katayama, T.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学角間キャンパス(石川県金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mechanism of Triplex DNA-Binding Proteins to Recognize Triplex DNA2014

    • Author(s)
      Kiuchi, K., Mase, K., Sato, N., Katayama, T., Torigoe, H.
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Molecular Mechanism of Triplex DNA-Binding Proteins to Recognize Triplex DNA2014

    • Author(s)
      Torigoe, H., Kiuchi, K., Mase, K., Sato, N., Katayama, T.
    • Organizer
      The 41st International Symposium on Nucleic Acids Chemistry 2014
    • Place of Presentation
      北九州国際会議場(福岡県北九州市)
    • Year and Date
      2014-11-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Promotion of Antiparallel Triplex DNA Formation by Budding Yeast Triplex DNA Binding Protein Stm12014

    • Author(s)
      Kiuchi, K., Mase, K., Sato, N., Katayama, T., Torigoe, H.
    • Organizer
      The 41st International Symposium on Nucleic Acids Chemistry 2014
    • Place of Presentation
      北九州国際会議場(福岡県北九州市)
    • Year and Date
      2014-11-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 3本鎖DNA結合蛋白質の3本鎖DNA認識機構の解析2014

    • Author(s)
      木内一樹、間瀬貴久江、佐藤憲大、片山拓馬、鳥越秀峰
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2014-10-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 3本鎖DNA結合蛋白質の3本鎖DNA認識機構2014

    • Author(s)
      木内 一樹、間瀬 貴久江、鳥越 秀峰
    • Organizer
      第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山大学津島キャンパス(岡山県岡山市)
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 3本鎖DNA結合蛋白質の3本鎖DNA認識機構2014

    • Author(s)
      木内一樹、間瀬貴久江、佐藤憲大、片山拓馬、鳥越秀峰
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      ワークピア横浜/横浜産貿ホールマリネリア(神奈川県横浜市)
    • Year and Date
      2014-06-26
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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