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Development of Immunoassay Technology with Fine Organic Chemistry and Antibody Engineering

Research Project

Project/Area Number 26288087
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Green/Environmental chemistry
Research InstitutionToyohashi University of Technology

Principal Investigator

IWASA SEIJI  豊橋技術科学大学, 工学(系)研究科(研究院), 教授 (30303712)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords残留農薬分析 / 環境分析 / 抗原抗体反応 / イムノアッセイ法 / ELISA法 / 触媒 / カルベン / イムノクロマト法 / イムノアッセイ / ハプテン / 抗体 / 残留農薬 / 検査キット / リンカー / ネオニコチノイド / 遷移金属触媒 / イムノクロマトグラフィー / 免疫 / 残留農薬検査 / ハプテン合成 / 有機合成 / 免疫化学反応 / パラジウム / ルテニウム
Outline of Final Research Achievements

To keep food safety and the reliance, the development of a residue analysis of low molecular agricultural chemicals is one of most important social requirements as for both human health and environmental consideration. For this regard, our objective of this research project is to develop a simple, rapid and low cost performance analytical method with immunoassay being comparable to conventional instrumental analysis such as HPLC, GC, MASS and their combination. During this research project, we have established pesticide residue analysis with immunoassay method in the range of ppb. The key technology of immunoassay is the preparation of highly selective antibody to recognize the target pesticide residue. The catalyst was newly developed to introduce suitable linker on the pesticide and also apply carbentranfer reaction of diazo-comopunds. This system is simple, rapid, cost-effective, high mobility and high throughput analytical method and now commercially available.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (26 results)

All 2017 2016 2015 2014

All Journal Article (9 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 8 results,  Open Access: 6 results) Presentation (16 results) (of which Int'l Joint Research: 6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Ru(II)-Pheox-catalyzed Si-H insertion reaction: construction of enantioenriched carbon and silicon centers2017

    • Author(s)
      Yoko Nakagawa, Soda Chanthamath, Kazutaka Shibatomi, Seiji Iwasa
    • Journal Title

      Chemical Communications

      Volume: 53 Issue: 26 Pages: 3753-3756

    • DOI

      10.1039/c7cc01070b

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Inter- and Intramolecular Cyclopropanations of Diazo Weinreb Amides Catalyzed by Ruthenium(II)-Amm-Pheox2017

    • Author(s)
      Hamada, M, M.; Chanthamath, S.; Shibatomi, K.; Iwasa, S.
    • Journal Title

      Advanced Synthesis & Catalysis

      Volume: 359 Issue: 10 Pages: 1-6

    • DOI

      10.1002/adsc.201601345

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Enantioselective Cyclopropanation of a Wide Variety of Olefins Catalyzed by Ru(II)-Pheox Complexes2016

    • Author(s)
      Chanthamath, S.; Iwasa, S.
    • Journal Title

      Account of Chemical Research

      Volume: 46 Issue: 10 Pages: 2080-2090

    • DOI

      10.1021/acs.accounts.6b00070

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Development of an Immunosensor for Determination of the Fungicide Chlorothalonil in Vegetables, using Surface Plasmon Resonance2015

    • Author(s)
      Hirakawa, Y., Yamasaki, T.; Watanabe, E.; Okazaki, F.; Murakami-Y., Y.; Oda, M.; Iwasa, S.; Narita, H.; Miyake, S.,
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 63 Issue: 28 Pages: 6325-6330

    • DOI

      10.1021/acs.jafc.5b01980

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Analysis of the Fungicide Boscalid in Horticultural Crops Using an Enzyme-Linked Immunosorbent Assay and an Immunosensor Based on Surface Plasmon Resonance2015

    • Author(s)
      Hirakawa, Y.; Yamasaki, T.; Harada, A.; Ohtake, T.; Adachi, K.; Iwasa, S.; Narita, H.; Miyake, S.
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 63 Issue: 36 Pages: 8075-8082

    • DOI

      10.1021/acs.jafc.5b03637

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Highly Stereoselective Intramolecular Cyclopropanation Reactions of α, β-Unsaturated Esters Catalyzed by Chiral Ru(II)-Pheox Complex and their Application for the Synthesis of Medicinal Compounds2015

    • Author(s)
      Nakagawa, Y.; Chanthamath, S.; Shibatomi, K.; Iwasa, S.
    • Journal Title

      Organic Letters

      Volume: 17 Issue: 11 Pages: 2792-2795

    • DOI

      10.1021/acs.orglett.5b01201

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Development of Direct Competitive ELISA for Residue Analysis of Fungicide Chlorothalonil in Vegetables2014

    • Author(s)
      Okazaki, F.; Hirakawa, Y.; Yamaguchi (Murakami), Y.; Harada, A.; Watanabe, E.; Iwasa, S.; Narita, H.; Miyake, S.
    • Journal Title

      Food Hygiene and Safety Science

      Volume: 55 Pages: 65-72

    • NAID

      130004504405

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Highly Stereoselective Synthesis of Cyclopropylphosphonates Catalyzed by Chiral Ru(II)-Pheox Complex2014

    • Author(s)
      Chanthamath, S.; Ozaki, S,; Shibatomi, K.; Iwasa, S.
    • Journal Title

      Organic Letters

      Volume: 16 Issue: 11 Pages: 3012-3015

    • DOI

      10.1021/ol501135p

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly Regio- and Stereoselective Synthesis of Alkylidenecyclopropanes via Ru(II)-Pheox Catalyzed Asymmetric Inter- and Intramolecular Cyclopropanation of Allenes2014

    • Author(s)
      Chanthamath, S.; Chua, H. W.; Kimura, S.; Shibatomi, K.; Iwasa, S.
    • Journal Title

      Organic Letters

      Volume: 16 Issue: 12 Pages: 3408-3411

    • DOI

      10.1021/ol5014944

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Ru(II)-Pheox 触媒によるジアゾオキシインドール類のアルケン類への高立体選択的不斉シクロプロパン化2017

    • Author(s)
      刀根 雅也・CHANTHAMATH Soda・柴富 一孝・岩佐 精二
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応大学日吉キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Ru(II)-Pheox 触媒によるジアゾアセテート類のアミン-ボラン及びホスフィン-ボランへの高エナンチオ選択的B-H挿入反応2017

    • Author(s)
      オトグ ナンサルマー・CHANTHAMATH Soda・柴富 一孝・岩佐 精二
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応大学日吉キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Inter- and intramolecular cyclopropanations of diazo Weinreb amides catalyzed by Ru(II)-Amm-Pheox2017

    • Author(s)
      MANDOUR, Hamada S. A.; CHANTHAMATH, Soda; SHIBATOMI, Kazutaka; IWASA, Seiji
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応大学日吉キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of simultaneous chiral induction reactions on silicon and the neighboring carbon via catalytic asymmetric Si-H insertion and its computational chemical analysis2017

    • Author(s)
      4.NAKAGAWA, Yoko; CHANTHAMATH, Soda; NAKAYAMA, Naofumi; GOTO, Hitoshi; SHIBATOMI, Kazutaka; IWASA, Seiji
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶応大学日吉キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Ru(II)-Pheox catalyzed enantioselective Si-H insertion reaction of diazoacetates2016

    • Author(s)
      Y. Nakagawa, S. Chanthamath, K. Shibatomi and S. Iwasa
    • Organizer
      252nd ACS National Meeting & Exposition
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Highly Enantioselective C-H Insertion Reaction of N-Alkyldiazoacetamides by using Ru(II)-Pheox Complex2015

    • Author(s)
      Doan, T. T. T.; Chanthamath, S.; Shibatomi, K.; Iwasa, S.
    • Organizer
      Pacifichem 2015 (The 2015 International Chemical Congress of Pacific Basin Societies)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Highly enantioselective synthesis of cyclopropane ring fused γ-lactones via intramolecular carbene transfer reactions2015

    • Author(s)
      Y. Nakagawa, S. Chanthamath, N. Nakayama, H. Goto, K. Shibatomi and S. Iwasa
    • Organizer
      Pacifichem 2015 (The 2015 International Chemical Congress of Pacific Basin Societies)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ru(II)-Pheox catalyzed carbene transfer reactions of diethyldiazomethylphosphonate2015

    • Author(s)
      Seiya Ozaki, Soda Chanthamath, Kazutaka Shibatomi, Seiji Iwasa
    • Organizer
      Pacifichem 2015 (The 2015 International Chemical Congress of Pacific Basin Societies)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Highly Stereoselective Cyclopropanations of Weinreb Amide Derivatives using Novel Ru(II)-Pheox Catalysts2015

    • Author(s)
      Hamada S. A. Mandour, Soda Chanthamath, Kazutaka Shibatomi, Seiji Iwasa
    • Organizer
      Pacifichem 2015 (The 2015 International Chemical Congress of Pacific Basin Societies)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ru(II)-Pheox 触媒を用いたヘテロシクロオレフィン類への立体選択的な不斉シクロフプロパン化反応2015

    • Author(s)
      Batbuyan Munkhod,Soda Chanthamath,柴富一孝,岩佐精二
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      津,三重大
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Ru(II)-Pheox を用いるジアゾ化合物の不斉分子内 C-H 挿入反応2015

    • Author(s)
      渡邉 翔,岩佐精二,Soda Chanthamath
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      津,三重大
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Ru(II)-Pheox 触媒による Si-H 挿入反応を経由する有機ケイ素化合物の合成2015

    • Author(s)
      瀧本大晃,Soda Chanthamath,柴富一孝,岩佐精二
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      津,三重大
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Ru(II)-Pheox触媒によるジアゾアセテート類の高立体選択的不斉Si-H挿入反応2015

    • Author(s)
      中川陽子,田﨑正貴,CHANTHAMATH SODA,柴富一孝,岩佐精二
    • Organizer
      The 5th CSJ Chemistry Festal
    • Place of Presentation
      東京、タワーホール船堀
    • Year and Date
      2015-10-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Computational Chemical Analysis for Ru(II)-Pheox Catalyzed Enantioselective Intramolecular Cyclopropanation2015

    • Author(s)
      Nakagawa, Y.; Chanthamath, S.; Nakayama, N.; Goto, H.; Shibatomi, K.; Iwasa, S.
    • Organizer
      250th ACS National Meeting & Exposition, American Chemical Society
    • Place of Presentation
      MA, USA
    • Year and Date
      2015-08-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Highly Stereoselective Synthesis of trans- and cis-Cinnamic Weinreb Amides via 1,2-Hydride Migration2014

    • Author(s)
      S. Ozaki, S. Chanthamath, K. Shibatomi and S. Iwasa,
    • Organizer
      nternational Conference of Global Network for Innovative Technology
    • Place of Presentation
      Penang, Malaysia
    • Year and Date
      2014-12-12 – 2014-12-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Ru(II)-Pheox Catalyzed Asymmetric Cyclopropanation of Diethyl Diazomethylphosphonate with Various Alkenes2014

    • Author(s)
      S. Chanthamath, S. Ozaki, K. Shibatomi and S. Iwasa
    • Organizer
      XXVI International Conference of Organometallic Chemistry(ICOMC 2014)
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2014-07-13 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 抗ボスカリド抗体および該抗体を用いたボスカリド測定方法2015

    • Inventor(s)
      岩佐精二,三宅司郎、岩佐香代、
    • Industrial Property Rights Holder
      岩佐精二,三宅司郎、岩佐香代、
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-017356
    • Filing Date
      2015-01-30
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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