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Application of minute biofluid handling techniques using an artificial cell membrane

Research Project

Project/Area Number 26289038
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Fluid engineering
Research InstitutionChuo University

Principal Investigator

Suzuki Hiroaki  中央大学, 理工学部, 教授 (20372427)

Co-Investigator(Renkei-kenkyūsha) OKANO Taiji  中央大学, 理工学部, 助教 (60622082)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Keywordsバイオリアクター / リポソーム / 膜融合 / 膜分裂 / ゲノム増幅 / PCR / マイクロリアクター / ジャイアントリポソーム / 人工細胞膜 / 溶液操作 / マイクロ流体デバイス / 融合 / 分裂 / フェムトリットル / 反応場
Outline of Final Research Achievements

We aimed to establish an ultra-small biochemical assay platform using giant liposomes as a femtoliter-volume reactor, which enables addition, mixing, and aliquoting. As a means of GUV fusion, we studied PEG-mediated fusion method in addition to the well-established GUV electrofusion method, and found that PEG-fusion is more suitable for fusing GUV and the live cell membrane. For aliquoting, we thoroughly studied the effect of depletion-volume effect and addition of mono-acyl lipids. Finally we demonstrated the three distinct biochemical reactions in GUVs; (1) an enzyme reaction, (2) the whole-genome amplification, and (3) the reverse transcription and polymerase chain reaction (RT-PCR) to show that the sequential handling of these reagents is possible.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Book (2 results)

  • [Journal Article] Shape Transformation of lipid vesicles by insertion of bulky-head lipids2015

    • Author(s)
      S. Tsuda, S. Fujii, H. Suzuki, T. Yomo
    • Journal Title

      Plos One

      Volume: - Issue: 7 Pages: e0132963-e0132963

    • DOI

      10.1371/journal.pone.0132963

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Stochasticity in Gene Expression in a Cell-Sized Compartment2015

    • Author(s)
      Kz. Nishimura, T. Mtasuura, T. Sunami, S. Tsuru, H. Suzuki, T. Yomo
    • Journal Title

      ACS Synthetic Biology

      Volume: 4 Issue: 5 Pages: 566-576

    • DOI

      10.1021/sb500249g

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of giant unilamellar vesicles with permeability to small charged molecules2014

    • Author(s)
      Nishimura, K., Matsuura, T., Sunami, T., Fujii, S., Nishimura, K., Suzuki, H., and Yomo, T.
    • Journal Title

      Rsc Adv

      Volume: 4(66) Issue: 66 Pages: 35224-35232

    • DOI

      10.1039/c4ra05332j

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Liposome-based liquid handling platform featuring addition, mixing, and aliquoting of femtoliter volumes2014

    • Author(s)
      H. Shiomi, S. Tsuda, H. Suzuki, T. Yomo
    • Journal Title

      Plos One

      Volume: なし Issue: 7 Pages: e101820-e101820

    • DOI

      10.1371/journal.pone.0101820

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Statistical analysis of vesicle morphology dynamics based on free energy landscape2014

    • Author(s)
      S. Tsuda, H. Suzuki, T. Yomo
    • Journal Title

      Soft Matter

      Volume: 10 Issue: 32 Pages: 6038-6046

    • DOI

      10.1039/c4sm00992d

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 人工細胞膜システムの細胞らしい挙動2017

    • Author(s)
      鈴木宏明
    • Organizer
      生体界面研究会
    • Place of Presentation
      金沢
    • Year and Date
      2017-02-09
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] リポソームを用いたサブピコリットル反応液への試薬供給2016

    • Author(s)
      岡野太治,鈴木宏明
    • Organizer
      第33回「センサ・マイクロマシンと応用システム」シンポジウム
    • Place of Presentation
      長崎
    • Year and Date
      2016-10-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] ハイドロゲル内封ユニラメラリポソームの作製法の検討2016

    • Author(s)
      勝田翔太,岡野太治,鈴木宏明
    • Organizer
      第34回化学とマイクロナノシステム学会
    • Place of Presentation
      千葉
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 人工細胞系構築の試み2016

    • Author(s)
      鈴木宏明
    • Organizer
      生体界面研究会
    • Place of Presentation
      愛知
    • Year and Date
      2016-07-04
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 分裂する人工細胞膜におけるゲノムDNAの分配機構2016

    • Author(s)
      鈴木宏明
    • Organizer
      第33回化学とマイクロナノシステム学会
    • Place of Presentation
      東京
    • Year and Date
      2016-04-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 分裂する人工細胞膜におけるゲノムDNAの分配機構2016

    • Author(s)
      鈴木宏明
    • Organizer
      化学とマイクロ・ナノシステム学会 第33回研究会
    • Place of Presentation
      東京大学生産技術研究所
    • Year and Date
      2016-04-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] 人工細胞膜システムの細胞らしい挙動2016

    • Author(s)
      鈴木宏明
    • Organizer
      東京理科大研究推進機構総合研究院 イメージングフロンティアセンターシンポジウム
    • Place of Presentation
      千葉
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 細胞の界面をつくる・制御する2016

    • Author(s)
      鈴木宏明
    • Organizer
      日本表面化学会関東支部第5回セミナー 東京理科大学ウォーターフロンティアサイエンスシンポジウム
    • Place of Presentation
      東京
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 機械(メカ)屋からみた細胞~細胞はつくれるか?~2016

    • Author(s)
      鈴木宏明
    • Organizer
      ナノ茶論
    • Place of Presentation
      神奈川
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Distribution of genome-size DNA in the dividing model cell membrane2015

    • Author(s)
      Hiroaki Suzuki
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Liquid handling of minute volume using the hydrogel encapsulating liposomes2015

    • Author(s)
      K. Takahashi, T. Okano, H. Suzuki
    • Organizer
      The 19th international conference on miniaturized systems for chemistry and life sciences
    • Place of Presentation
      Geongju, Korea
    • Year and Date
      2015-10-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ハイドロゲル内封リポソームを用いた微小溶液操作系の構築2014

    • Author(s)
      高橋和志,岡野太治,鈴木宏明
    • Organizer
      化学とマイクロ・ナノシステム学会 第30回研究会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-10-02 – 2014-10-03
    • Related Report
      2014 Annual Research Report
  • [Book] 少数生生物学(第10章 少数を分けるー細胞膜中の分子の離散生と分配)2017

    • Author(s)
      鈴木宏明(分担執筆)
    • Total Pages
      192
    • Publisher
      日本評論社
    • Related Report
      2016 Annual Research Report
  • [Book] 人工細胞の創製とその応用(2.1 人工細胞の容器としてのリポソーム)2016

    • Author(s)
      岡野太治,鈴木宏明(分担執筆)
    • Total Pages
      215
    • Publisher
      シーエムシー出版
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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