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Development of DNA diagnosis device using dielectrophoretic impedance measurement

Research Project

Project/Area Number 26289125
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Measurement engineering
Research InstitutionKyushu University

Principal Investigator

Suehiro Junya  九州大学, システム情報科学研究科(研究院, 教授 (70206382)

Co-Investigator(Renkei-kenkyūsha) NAKANO Michihiko  九州大学, 大学院システム情報科学研究院, 准教授 (00447856)
Research Collaborator DING Zhenhao  九州大学, 大学院システム情報科学府
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords誘電泳動 / DNA / 細菌検査 / 食品検査 / Bio MEMS / 電気インピーダンス / インピーダンス / PCR / 計測機器 / 遺伝子検査 / 細菌検出 / DNA診断 / マイクロビーズ
Outline of Final Research Achievements

DEPIM (dielectrophoretic impedance measurement) revealed that the microelectrode conductance increased with elapsed time as a result of positive DEP trap of DNA-labeled microbeads. On the other hand, intact microbeads were not trapped on the microelectrode decreasing the conductance. This result implies that the presented DNA detection method can basically works as intended.
The electrical DNA detection method was further applied to bacterial detection. Bacterial DNA was extracted from E. coli strain K12 bacteria and amplified by polymerase chain reaction (PCR). The presented method realized quantitative detection of specific bacteria at concentrations above 100000 and 24000 CFU/ml for bacterial solutions with and without other bacterial presence, respectively. It was also showed the method detected 100000 copies of pig meat DNA in the mixture of 10<sup>8</sup> copies of cow meat DNA. As the results, 5 mg of pork in 50 mg of meat mixed with beef was detected.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(39 results)

All 2017 2016 2015 2014 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 8 results) Presentation (29 results) (of which Int'l Joint Research: 5 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Bacterial detection based on polymerase chain reaction and microbead dielectrophoresis characteristics2017

    • Author(s)
      Z. Ding, H. Kasahara, M. Nakano, J. Suehiro
    • Journal Title

      IET Nanobiotechnology

      Volume: 印刷中 Pages: 562-567

    • DOI

      10.1049/iet-nbt.2016.0186

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 微粒子誘電泳動を用いたDNA検出法による豚DNAの選択的検出2017

    • Author(s)
      山崎 達郎、丁 震昊、中野 道彦、末廣 純也
    • Journal Title

      静電気学会誌

      Volume: 41

    • NAID

      40021341130

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] DNA結合に伴う誘電体微粒子の誘電泳動特性変化の測定2016

    • Author(s)
      中野 道彦, 笠原 弘道, 丁 震昊, 末廣 純也
    • Journal Title

      静電気学会誌

      Volume: 40 Pages: 20-25

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads2015

    • Author(s)
      H. Kasahara, Z. Ding, M. Nakano, J. Suehiro
    • Journal Title

      Proceedings of the IEEE International Conference on Industrial Technology

      Volume: 2015-June Pages: 3341-3346

    • DOI

      10.1109/icit.2015.7125593

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Concentration of bacteria in high conductive medium using negative dielectrophoresis2015

    • Author(s)
      Y. Inoue, R. Obara, M. Nakano, J. Suehiro
    • Journal Title

      Proceedings of the IEEE International Conference on Industrial Technology

      Volume: 2015-June Pages: 3336-3340

    • DOI

      10.1109/icit.2015.7125592

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy2015

    • Author(s)
      M. Nakano, Z. Ding, H. Kasahara, J. Suehiro
    • Journal Title

      Proceedings of Transducers 2015

      Volume: なし Pages: 1-4

    • DOI

      10.1109/transducers.2015.7181228

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement2015

    • Author(s)
      M. Nakano, H. Kasahara, Z. Ding, J. Suehiro
    • Journal Title

      Proceedings of IEEE Sensors 2015

      Volume: なし Pages: 1-4

    • DOI

      10.1109/icsens.2015.7370216

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rapid microbead-based DNA detection using dielectrophoresis and impedance measurement2014

    • Author(s)
      Michihiko Nakano, Zhenhao Ding, Hiromichi Kasahara, and Junya Suehiro
    • Journal Title

      EPL

      Volume: 108 Pages: 28003-28003

    • DOI

      10.1209/0295-5075/108/28003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 耐熱性アビジン修飾微粒子を用いた誘電泳動によるDNA検出2017

    • Author(s)
      山崎 達郎、丁 震昊、堀田 博一、中野 道彦、末廣 純也
    • Organizer
      電気学会全国大会
    • Place of Presentation
      富山大学
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 微粒子誘電泳動を用いた混合肉試料からの豚DNAの選択的検出2016

    • Author(s)
      山崎 達郎、丁 震昊、中野 道彦、末廣 純也
    • Organizer
      電気・情報関係学会九州支部第69回連合大会
    • Place of Presentation
      宮崎大学
    • Year and Date
      2016-09-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] Advances in DNA detection method based on microbeads dielectrophoresis2016

    • Author(s)
      M. Nakano, T. Yamasaki, Z. Ding, J. Suehiro
    • Organizer
      Dielectrophoresis 2016
    • Place of Presentation
      MIT, USA
    • Year and Date
      2016-07-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微粒子誘電泳動を用いたDNA検出法による豚由来DNAの選択的検出2016

    • Author(s)
      山崎 達郎、丁 震昊、中野 道彦、末廣 純也
    • Organizer
      電気学会センサ・マイクロマシン部門総合研究会
    • Place of Presentation
      金沢市文化ホール
    • Year and Date
      2016-06-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] Quantitative bacterial detection using rapid DNA detection method based on microbeads dielectrophoresis2016

    • Author(s)
      Z.H. Ding, H. Kasahara, M. Nakano, J. Suehiro
    • Organizer
      Biosensors 2016
    • Place of Presentation
      Gothenburg, Sweden
    • Year and Date
      2016-05-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of DNA length on dielectrophoresis of DNA-labeled microbeads: Crossover frequency and zeta potential2016

    • Author(s)
      M. Nakano, H. Kasahara, Z. Ding, J. Suehiro
    • Organizer
      Biosensors 2016
    • Place of Presentation
      Gothenburg, Sweden
    • Year and Date
      2016-05-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] , Sensitive and Quantitative DNA Detection by Beads-based Dielectrophoretic Impedance Measurement2015

    • Author(s)
      Michihiko Nakano, Hiromichi Kasahara, Zhenhao Ding, Junya Suehiro
    • Organizer
      IEEE SENSORS 2015
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2015-11-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] リアルタイムPCRとの比較による微粒子誘電泳動を用いたDNA検出法の定量性評価2015

    • Author(s)
      中野 道彦, 丁 震昊, 笠原弘道, 末廣 純也
    • Organizer
      平成27年度(第68回)電気・情報関係学会 九州支部連合大会
    • Place of Presentation
      福岡大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] PCRと微粒子誘電泳動を用いたDNA検出法による細菌検出2015

    • Author(s)
      丁 震昊, 笠原弘道, 中野 道彦, 末廣 純也
    • Organizer
      平成27年度(第68回)電気・情報関係学会 九州支部連合大会
    • Place of Presentation
      福岡大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 負の誘電泳動現象を利用した高感度DNA検出用デバイスの提案2015

    • Author(s)
      井上祐樹, 中野 道彦, 末廣 純也
    • Organizer
      平成27年度(第68回)電気・情報関係学会 九州支部連合大会
    • Place of Presentation
      福岡大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 微粒子誘電泳動を用いたDNA検出法のDNA結合条件の影響2015

    • Author(s)
      笠原弘道, 丁 震昊, 中野 道彦, 末廣 純也
    • Organizer
      平成27年度(第68回)電気・情報関係学会 九州支部連合大会
    • Place of Presentation
      福岡大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] DNA 結合に伴う誘電体微粒子の誘電泳動特性変化の測定, , 2015.09.24.2015

    • Author(s)
      中野 道彦, 笠原弘道, 丁 震昊, 末廣 純也
    • Organizer
      第39回静電気学会全国大会
    • Place of Presentation
      首都大学東京
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 負の誘電泳動力による細菌濃縮を利用した誘電泳動インピーダンス計測法の高感度化2015

    • Author(s)
      井上祐樹, 中野 道彦, 末廣 純也
    • Organizer
      電気学会 センサ・マイクロマシン部門総合研究会
    • Place of Presentation
      九州大学
    • Year and Date
      2015-07-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] 微粒子の誘電泳動を利用したDNA高速検出法の検出感度調査2015

    • Author(s)
      笠原弘道, 丁震昊, 中野 道彦, 末廣 純也
    • Organizer
      電気学会 センサ・マイクロマシン部門総合研究会
    • Place of Presentation
      九州大学
    • Year and Date
      2015-07-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] 誘電泳動現象を利用した迅速DNA検出法の細菌検出への応用2015

    • Author(s)
      丁震昊, 笠原弘道, 中野 道彦, 末廣 純也
    • Organizer
      電気学会 センサ・マイクロマシン部門総合研究会
    • Place of Presentation
      九州大学
    • Year and Date
      2015-07-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Rapid Size Determination of PCR Amplifed DNA by Beads-Based Dielectrphoretic Impedance Spectroscopy2015

    • Author(s)
      M. Nakano, Z. Ding, H. Kasahara, and J. Suehiro
    • Organizer
      Transducers 2015
    • Place of Presentation
      Dena'ina Convention Center, USA
    • Year and Date
      2015-06-21 – 2015-06-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] RAPID SIZE DETERMINATION OF PCR AMPLIFIED DNA BY BEADS-BASED DIELECTRPHORETIC IMPEDANCE SPECTROSCOPY2015

    • Author(s)
      M. Nakano, Z. Ding, H. Kasahara, and J. Suehiro
    • Organizer
      The 18the International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015)
    • Place of Presentation
      Dena'ina Convention Center, Alaska, USA
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA修飾粒子の誘電泳動特性:粒子径の影響2015

    • Author(s)
      笠原弘道、Nur Afila Binti Abd Latif、丁 震昊、中野道彦、末廣純也
    • Organizer
      平成27年電気学会全国大会
    • Place of Presentation
      東京都市大学 世田谷キャンパス
    • Year and Date
      2015-03-24 – 2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effect of DNA length on dielectrophoretic characteristics of DNA-labeled microbeads2015

    • Author(s)
      H. Kasahara, Z. Ding, M. Nakano, J. Suehiro
    • Organizer
      2015 IEEE International Conference on Industrial Technology
    • Place of Presentation
      Hotel Meli&#225; Sevilla, Spain
    • Year and Date
      2015-03-17 – 2015-03-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dielectrophoretic Characteristics of Microbeads Labeled with DNA of Various Lengths2015

    • Author(s)
      Z. Ding, H. Kasahara, M. Nakano and J. Suehiro
    • Organizer
      Biodevices 2015
    • Place of Presentation
      Lisbon Marriott Hotel, Portugal
    • Year and Date
      2015-01-12 – 2015-01-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA Detection Using Microbeads-Based Dielectrophoretic Impedance Measurement2014

    • Author(s)
      M. Nakano, Z. Ding, H. Kasahara, J. Suehiro
    • Organizer
      IEEE SENSORS 2014
    • Place of Presentation
      Valencia Conference Centre, Spain
    • Year and Date
      2014-11-03 – 2014-11-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Selective Detection of DNA with Different Length Using Microbeads-Based Dielectrophoresis and Impedance Measurement2014

    • Author(s)
      M. Nakano, H. Kasahara, Z. Ding, and J. Suehiro
    • Organizer
      MicroTAS 2014
    • Place of Presentation
      Henry B. Gonzalez Convention Center, USA
    • Year and Date
      2014-10-26 – 2014-10-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 表面をDNA修飾した微粒子の誘電泳動特性評価2014

    • Author(s)
      笠原弘道,丁震昊,中野道彦,末廣純也
    • Organizer
      第31回「センサ・マイクロマシンと応用システム」シンポジウム
    • Place of Presentation
      くにびきメッセ
    • Year and Date
      2014-10-20 – 2014-10-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA修飾微粒子の誘電泳動に及ぼす塩基対数の影響2014

    • Author(s)
      笠原弘道, 丁晨昊, 中野道彦, 末廣純也
    • Organizer
      平成26年度(第67回)電気・情報関係学会九州支部連合大会
    • Place of Presentation
      鹿児島大学 工学部
    • Year and Date
      2014-09-18 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA結合に伴う微粒子の誘電泳動変化を利用したDNA検出2014

    • Author(s)
      中野道彦, 丁震昊, 笠原弘道, 末廣純也
    • Organizer
      第38回静電気学会全国大会
    • Place of Presentation
      広島国際大学 呉キャンパス
    • Year and Date
      2014-09-08 – 2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rapid DNA Detection by Microbead based Dielectrophoretic Impedance Measurement with Modified Voltage Waveform2014

    • Author(s)
      M. Nakano, Z. Ding, H. Kasahara, R. Obara and J. Suehiro
    • Organizer
      The 15th IUMRS-International Conference in Asia
    • Place of Presentation
      Fukuoka University, Japan
    • Year and Date
      2014-08-24 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dielectrophoretic characteristics of DNA-labeled microbeads with various ratios of DNA to microbeads2014

    • Author(s)
      Z. Ding, H. Kasahara, R. Obara, Y. Inoue, M. Nakano and J. Suehiro
    • Organizer
      Dielectrophoresis 2014
    • Place of Presentation
      Institute of Physics, U.K.
    • Year and Date
      2014-07-14 – 2014-07-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rapid DNA detection based on dierection reversing of dielectrophoresis of DNA-attached microbeads2014

    • Author(s)
      M. Nakano, Z. Ding, R. Obara, H. Kasahara, J. Suehiro
    • Organizer
      BIOSENSORS 2014
    • Place of Presentation
      Melbourne Convention and Exhibition Centre, Australia
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effects of DNA Labeling on Dielectrophoretic Characteristics of Microbeads2014

    • Author(s)
      Z. Ding, H. Kasahara, R. Obara, M. Nakano, J. Suehiro
    • Organizer
      ADVANCES IN MICROFLUIDICS & NANOFLUIDICS 2014
    • Place of Presentation
      ACADEMIA SINICA, Taiwan
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Remarks] 末廣・中野研ホームページ

    • URL

      http://hv.ees.kyushu-u.ac.jp/Lab-j/index.html

    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 核酸検出法2014

    • Inventor(s)
      中野 道彦、末廣 純也
    • Industrial Property Rights Holder
      中野 道彦、末廣 純也
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-183933
    • Filing Date
      2014-09-10
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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