Targeting of cancer cells by protein nanospheres with high functions

• Project/Area Number: 26289310
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Kobatake Eiry 東京工業大学, 生命理工学院, 教授 (00225484)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: タンパク質 / ナノ粒子 / DDS / 抗がん / 生体機能材料 / バイオテクノロジー / 生体分子 / ナノバイオ / 生体機能利用 / 蛋白質

Outline of Final Research Achievements

In this study, protein nanospheres were constructed for drug delivery system (DDS). Component proteins were consisting of hydrophobic thermo-responsive protein and poly-aspartic acids. This protein could be assembled and formed nanoapheres by mild heating. Molecular recognizing functional proteins could be displayed on the surface of nanospheres. On the other hand, hydrophobic anti-cancer drugs could be incorporated into the inside of nanospheres. These modified nanosphres could recognized cancer cells specifically, and kill cells by releasing anti-cancer drugs.

Research Products

• [Journal Article] Design of bFGF-tethered self-assembling extracellular matrix proteins via coiled-coil triple-helix formation (2017)
  • Author(s): Mizuguchi Yoshinori、Mashimo Yasumasa、Mie Masayasu、Kobatake Eiry
  • Journal Title: Biomedical Materials Volume: 12 Issue: 4 Pages: 045021-045021
  • DOI: 10.1088/1748-605x/aa7616
• [Journal Article] Microfluidic-nanofiber hybrid array for screening of cellular microenvironments (2017)
  • Author(s): K. Kamei, Y. Mashimo, M. Yoshioka, Y. Tokunaga, C. Fockenberg, S. Terada, M. Nakajima, T. Shibata-Seki, L. Liu, T. Akaike, E. Kobatake, E. S. How, M. Uesugi and Y. Chen
  • Journal Title: Small Volume: 印刷中 Issue: 18 Pages: 1603104-1603104
  • DOI: 10.1002/smll.201603104
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation (2016)
  • Author(s): SokeLee Siew, Mami Kaneko, Masayasu Mie and Eiry Kobatake
  • Journal Title: J. Mater. Chem. B Volume: 4 Issue: 14 Pages: 2512-2518
  • DOI: 10.1039/c5tb01579k
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Construction of a defined biomimetic matrix for long-term maintenance of mouse induced pluripotent stem cells (2016)
  • Author(s): Nihad Adnan, Masayasu Mie, Amranul Haque, Sharif Hossain, Yasumasa Mashimo, Toshihiro Akaike, Eiry Kobatake
  • Journal Title: Construction of a defined biomimetic matrix for long-term maintenance of mouse induced pluripotent stem cells Volume: 27 Issue: 7 Pages: 1599-1605
  • DOI: 10.1021/acs.bioconjchem.6b00141
  • Peer Reviewed
• [Journal Article] Design of luciferase-displaying protein nanoparticles for use as highly sensitive immunoassay detection probes (2016)
  • Author(s): Yusuke Ikeda, Yasumasa Mashimo, Masayasu Mie, Eiry Kobatake
  • Journal Title: Analyst Volume: 141 Issue: 24 Pages: 6557-6563
  • DOI: 10.1039/c6an01253a
  • Peer Reviewed / Open Access
• [Journal Article] Growth factor tethering to protein nanoparticles via coied-coil foramation for targeted delivery. (2015)
  • Author(s): Ｙ．Ａｓｓａｌ，Ｙ．Ｍｉｚｕｇｕｃｈｉ， Ｍ． Ｍｉｅ， Ｅ． Ｋｏｂａｔａｋｅ
  • Journal Title: Ｂｉｏｃｏｎｊｕｇａｔｅ Ｃｈｅｍ． Volume: 26(8) Issue: 8 Pages: 1672-1677
  • DOI: 10.1021/acs.bioconjchem.5b00266
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Delivery of bFGF for tissue engineering by tethering to the ECM (2015)
  • Author(s): C. Suttinont,Y.Mashimo,M.Mie,E.Kobatake
  • Journal Title: Biomed．Ｒｅｓ．Ｉｎｔ． Volume: 1 Pages: 1-6
  • DOI: 10.1155/2015/208089
  • Peer Reviewed
• [Journal Article] Solubilization of Single-Walled Carbon Nanotubes using a Peptide Aptamer in Water below Critical Micelle Concentration (2015)
  • Author(s): Zha Li, Tomoshi Kameda, Takashi Isoshima, Eiry Kobatake, Takeshi Tanaka, Yoshihiro Ito, and Masuki Kawamoto
  • Journal Title: Langmuir Volume: 31 Issue: 11 Pages: 3482-3488
  • DOI: 10.1021/la504777b
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Targeting of EGF-displayed protein nanoparticles with anticancer drugs (2014)
  • Author(s): Matsumoto R, Hara R, Andou T, Mie M, Kobatake E
  • Journal Title: J Biomed Mater Res B Appl Biomater Volume: (in press) Issue: 8 Pages: 1792-1798
  • DOI: 10.1002/jbm.b.33162
  • Peer Reviewed
• [Journal Article] Cellular differentiation assessments by measuring the degree of cellular internalization and membrane adsorption using designed peptides (2014)
  • Author(s): K.Usui,T.Kikuchi,M.Mie,E.Kobatake,H.Mihara
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 24 Issue: 17 Pages: 4129-4131
  • DOI: 10.1016/j.bmcl.2014.07.053
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] DNA-based immunoassays for sensitive detection of protein (2014)
  • Author(s): F.Akter, M.Mie, E.Kobatake
  • Journal Title: Sensors and Actuators B Volume: 202 Pages: 1248-1256
  • DOI: 10.1016/j.snb.2014.05.135
  • Peer Reviewed
• [Presentation] Construction of DNA Aptamer Displayed Nanoparticles (2018)
  • Author(s): GUO Wei; MASHIMO, Yasumasa; MIE Masayasu; KOBATAKE, Eiry
  • Organizer: 日本化学会第98春季年会
• [Presentation] がん細胞を標的とした高機能化タンパク質ナノ粒子の開発 (2017)
  • Author(s): 池田裕介，眞下泰正，三重正和，小畠英理
  • Organizer: 第39回日本バイオマテリアル学会大会
• [Presentation] 転写因子タンパク質導入による細胞機能制御 (2017)
  • Author(s): 島村萌里，眞下泰正，三重正和，小畠英理
  • Organizer: 第39回日本バイオマテリアル学会大会
• [Presentation] 3次元細胞機能制御を指向した多機能タンパク質ハイドロゲルの構築 (2017)
  • Author(s): 水口佳紀、眞下泰正、三重正和、小畠英理
  • Organizer: つくば医工連携フォーラム2017
  • Place of Presentation: つくば市 物質・材料研究機構
• [Presentation] 高感度バイオセンシングに向けたタンパク質ナノ粒子の構築 (2016)
  • Author(s): 池田裕介、眞下泰正、三重正和、小畠英理
  • Organizer: 日本バイオマテリアル学会シンポジウム2016
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2016-11-21
• [Presentation] Construction of bFGF-tethered ECM for tissue engineering (2015)
  • Author(s): C.Suttinont, Y.Mashimo, M. Mie, E.Kobatake
  • Organizer: PACIFICHEM 2015
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Development of protein nanoparticles for sensitive biosensing (2015)
  • Author(s): Y. Ikeda, Y.Mashimo, M.Mie, E.Kobatake
  • Organizer: PACIFICHEM2015
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Ｃｏｎｓｔｒｕｃｔｉｏｎ ｏｆ ＤＮＡ－ｐｒｏｔｅｉｎ ｈｙｂｒｉｄ ｆｏｒ ｓｅｎｓｉｔｉｖｅ ｂｉｏｓｅｎｓｉｎｇ (2014)
  • Author(s): Ｅ. Ｋｏｂａｔａｋｅ
  • Organizer: ＩＣＳＳ２０１４
  • Place of Presentation: Hong Kong、Ｃｈｉｎａ
  • Year and Date: 2014-12-08 – 2014-12-11
  • Invited
• [Presentation] 高感度バイオセンシングを指向したルシフェラーゼ融合タンパク質ナノ粒子の構築 (2014)
  • Author(s): 池田裕介、三重正和、小畠英理
  • Organizer: 第8回バイオ関連化学シンポジウム
  • Place of Presentation: 岡山大学（岡山県・岡山市）
  • Year and Date: 2014-09-11 – 2014-09-13