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Skeletal muscle tissue engineering by magnetic force-based nanotechnology

Research Project

Project/Area Number 26289315
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionKyushu University

Principal Investigator

Ito Akira  九州大学, 工学(系)研究科(研究院), 准教授 (60345915)

Co-Investigator(Kenkyū-buntansha) 清水 一憲  名古屋大学, 工学(系)研究科(研究院), 准教授 (70402500)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywordsティッシュエンジニアリング / 骨格筋 / 再生医療 / iPS細胞 / 磁性ナノ粒子 / 薬剤スクリーニング / マイクロデバイス / 筋ジストロフィ / ナノバイオテクノロジー / 三次元培養 / 筋分化 / バイオテクノロジー / ナノバイオ
Outline of Final Research Achievements

In this study, we have developed a novel technology of skeletal muscle tissue engineering, designated "magnetic force-based tissue engineering (Mag-TE)", for regenerative medicine and drug discovery. The most important characteristic of skeletal muscle is force generation. We constructed three dimensional skeletal muscle tissues by Mag-TE technique and showed that the tissue-engineered skeletal muscles contracted and generated force upon electrical pulse stimulation. In the present study, 1) we explored a method of myogenic differentiation of human iPS cells; 2) we demonstrated that the contractility data is indispensable for evaluation of in vitro tissue-engineered skeletal muscle grafts; 3) we developed micro devices for high throughput screening of muscular disease drugs.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(23 results)

All 2017 2016 2015 2014 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 4 results) Presentation (15 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Remarks (4 results)

  • [Journal Article] In vitro drug testing based on contractile activity of C2C12 cells in an epigenetic drug model.2017

    • Author(s)
      Ikeda K, Ito A, Imada R, Sato M, Kawabe Y, Kamihira M
    • Journal Title

      Scientific Reports

      Volume: 7 Pages: 44570-44570

    • DOI

      10.1038/srep44570

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of heat stimulation and L-ascorbic acid 2-phosphate supplementation on myogenic differentiation of artificial skeletal muscle tissue constructs.2017

    • Author(s)
      Kazushi Ikeda, Akira Ito, Masanori Sato, Shota Kanno, Yoshinori Kawabe and Masamichi Kamihira
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine

      Volume: in press Pages: 1322-1331

    • DOI

      10.1002/term.2030

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Improved contractile force generation of tissue-engineered skeletal muscle constructs by IGF-I and Bcl-2 gene transfer with electrical pulse stimulation.2016

    • Author(s)
      Kazushi Ikeda, Akira Ito, Masanori Sato, Yoshinori Kawabe and Masamichi Kamihira
    • Journal Title

      Regenerative Therapy.

      Volume: 3 Pages: 38-44

    • DOI

      10.1016/j.reth.2015.12.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of type IV collagen on myogenic characteristics of IGF-I gene-engineered myoblast cells.2015

    • Author(s)
      Akira Ito, Masahiro Yamamoto, Kazushi Ikeda, Masanori Sato, Yoshinori Kawabe and Masamichi Kamihira
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 119 Issue: 5 Pages: 596-603

    • DOI

      10.1016/j.jbiosc.2014.10.008

    • ISSN
      1389-1723
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] カドヘリン遺伝子導入フィーダー細胞を用いたiPS細胞の運動神経分化誘導2017

    • Author(s)
      吉岡 貫太郎, 井藤 彰, 河邉 佳典, 上平 正道
    • Organizer
      化学工学会
    • Place of Presentation
      東京都
    • Related Report
      2016 Annual Research Report
  • [Presentation] 磁性ナノ粒子を用いたティッシュエンジニアリング技術の開発2016

    • Author(s)
      井藤 彰
    • Organizer
      日本機械学会 第28回バイオエンジニアリング講演会
    • Place of Presentation
      東京都
    • Year and Date
      2016-01-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] ティッシュエンジニアリングで作製した骨格筋の収縮力を指標としたin vitro薬剤試験2016

    • Author(s)
      井藤 彰, 池田一史, 今田隆介, 佐藤暢哲, 河邉佳典, 上平正道
    • Organizer
      化学工学会
    • Place of Presentation
      徳島県徳島市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 人工骨格筋組織の収縮力測定による薬剤評価系の構築2016

    • Author(s)
      池田 一史, 井藤 彰, 今田 隆介, 河邉 佳典, 上平 正道
    • Organizer
      日本生物工学会
    • Place of Presentation
      富山県富山市
    • Related Report
      2016 Annual Research Report
  • [Presentation] カドヘリン遺伝子導入フィーダー細胞を用いたiPS細胞の神経分化誘導2016

    • Author(s)
      ○(学)吉岡 貫太郎, Paerwen Paerhati, 井藤 彰, 山部 ひとみ, 岩本 佳央梨, 河邉 佳典, 上平 正道
    • Organizer
      化学工学会
    • Place of Presentation
      徳島県徳島市
    • Related Report
      2016 Annual Research Report
  • [Presentation] Magnetic Force-Based Skeletal Muscle Tissue Engineering for In Vitro Drug Testing2016

    • Author(s)
      Akira Ito, Kazushi Ikeda, Ryusuke Imada, Masanori Sato, Yoshinori Kawabe and Masamichi Kamihira
    • Organizer
      International Conference on Nanomedicine and Nanobiotechnology
    • Place of Presentation
      仏国パリ
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a Drug Testing System Based on Contractile Force Generation of Tissue-engineered Skeletal Muscle Constructs2016

    • Author(s)
      Kazushi Ikeda, Akira Ito, Ryusuke Imada, Yoshinori Kawabe, Masamichi Kamihira
    • Organizer
      JAACT2016
    • Place of Presentation
      神戸市
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cadherin Gene-engineered Feeder Systems for Neural Differentiation of Mouse iPS Cells2016

    • Author(s)
      Paerwen Paerhati, Kantaro Yoshioka, Kaori Iwamoto, Hitomi Yamabe, Akira Ito, Masamichi Kamihira, Yoshinori Kawabe
    • Organizer
      JAACT2016
    • Place of Presentation
      神戸市
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 磁気細胞操作によるティッシュエンジニアリング技術の開発2015

    • Author(s)
      井藤 彰
    • Organizer
      化学とマイクロ・ナノシステム学会
    • Place of Presentation
      北九州市
    • Year and Date
      2015-11-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Construction of a drug screening system using artificial skeletal muscle tissues2015

    • Author(s)
      Kazushi Ikeda, Akira Ito, Ryusuke Imada, Yoshinori Kawabe, Masamichi Kamihira
    • Organizer
      The 21th Symposium of Young Asian Biochemical Engineers' Community
    • Place of Presentation
      韓国 春川市
    • Year and Date
      2015-10-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 磁力を用いた骨格筋組織工学技術による薬剤探索系の開発2015

    • Author(s)
      池田一史, 井藤 彰, 今田 隆介, 河邉佳典, 上平正道
    • Organizer
      化学工学会第47回秋季大会
    • Place of Presentation
      札幌市
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] ティッシュエンジニアリングで作製した筋組織を用いた薬剤スクリーニング法の開発2015

    • Author(s)
      今田 隆介, 井藤 彰, 池田 一史, 河邉 佳典, 上平 正道
    • Organizer
      化学工学会第83年会
    • Place of Presentation
      芝浦工大豊洲キャンパス
    • Year and Date
      2015-03-19 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Artificial skeletal muscle tissues using follistatin gene-engineered myoblasts.2014

    • Author(s)
      Kazushi Ikeda, Akira Ito, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira
    • Organizer
      BMEiCON2014
    • Place of Presentation
      九州大学病院キャンパス
    • Year and Date
      2014-11-26 – 2014-11-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Fabrication of artificial skeletal muscle tissues using follistatin gene-engineered myoblasts.2014

    • Author(s)
      Kazushi Ikeda, Akira Ito, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira
    • Organizer
      JAACT2014
    • Place of Presentation
      北九州市小倉北区
    • Year and Date
      2014-11-11 – 2014-11-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] IGF-IおよびBcl-2の遺伝子共導入による人工骨格筋組織の機能強化2014

    • Author(s)
      池田 一史, 井藤 彰, 佐藤 暢哲, 河邉 佳典, 上平 正道
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Remarks] 九州大学工学研究院化学工学部門上平研究室HP

    • URL

      http://www.chem-eng.kyushu-u.ac.jp/lab3/researches.html#saisei

    • Related Report
      2015 Annual Research Report
  • [Remarks] 九州大学臨床研究倫理審査委員会の承認を受けた研究について

    • URL

      http://www.chem-eng.kyushu-u.ac.jp/lab3/download/rinsho.pdf

    • Related Report
      2015 Annual Research Report
  • [Remarks] 九州大学工学研究院化学工学部門上平研HP

    • URL

      http://www.chem-eng.kyushu-u.ac.jp/lab3/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 九州大学研究者情報

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002906/index.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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