| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
This study aimed to develop a technology to generate transgenic chickens derived from pluripotent stem cells as a new production platform for biopharmaceuticals. Total RNAs extracted from chicken blastodermal cells, containing pluripotent stem cells, were applied for the massive sequencing technology to analyze expression profiles of reprogramming factor genes. Using reprogramming factor genes identified by the transcriptome analysis, chicken induced pluripotent stem cells were generated. Furthermore, a genome editing technique, CRISPR/Cas9 system was applied for targeting a sex determination gene, DMRT1. A targeting vector designed efficiently altered the target region in DMRT1 gene of chicken cells.
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