| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Outline of Final Research Achievements |
In this research project, we have succeeded in developing an underwater atomic force microscope which can be operated in a deep sea environment in order to visualize microscopic microorganisms in deepwater with nanometer resolution. The underwater atomic force microscope is mounted on an remotely-operated underwater vehicle, and can be operated remotely from a reserach vessel to perform nanoscale imaging in deep sea. The observation samples in deepwater are remotely fixed using the sample holder equipped with a membrane filter, which have been invented in this project. The precise imaging in the deep sea was achieved by insulation coating of a self-sensitive cantilever with Parylene. The imaging was carried out in the deep sea at water depth from 600 to 2,000 m using this system. As a result, we have successfully achieved in-situ observation of a deep sea sample with a size of about 1 μm with a resolution of several 100 nm.
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