Mechanical and pathological analyses of shootin-mediated brain formation
Project/Area Number |
26290007
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
Naoyuki Inagaki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20223216)
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Co-Investigator(Renkei-kenkyūsha) |
URASAKI Akihiro (SAKUMURA Yuichi) 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40550083)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
Keywords | Shootin / 大脳組織形成 / ノックアウトマウス / ゼブラフィッシュ / メカノバイオロジー / 大脳皮質形成 |
Outline of Final Research Achievements |
Force interaction between cells and adhesive substreates induces cell migration that plays a key role in tissue morphogenesis. However, the molecular mechanics how cells generate forces between cells and substreates for cell migration and tissue morphogenesis remains unclear. Shootin1 is a brain-specific protein involved in axon formation. We found a novel splicing isoform of shootin1 which is expressed not only in the brain but also in peripheral tissues. We renamed the brain-specific shootin1 as shootin1a and termed the novel isoform as shootin1b. We found that shootin1 is involved in migration of olfactory neurons and formation of the olfactory bulb. Shootin1b interacts with actin filaments that undergo directional polymerization at the leading edge of migrating olfactory neurons. Our data suggest shootin1b mediates the linkage between the actin filaments and the cell adhesion molecule L1-CAM, thereby generating forces between cell’s leading and and substreates for migration.
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Report
(4 results)
Research Products
(21 results)
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[Presentation] In vivo function of singar which regulates polarity of cultured hippocampal neurons2015
Author(s)
Takuro, Kono., Hitomi, Nakazawa., Colleen, F Manning., James, S Trimmer., Kenji, Kono., Akihiro, Urasaki., Naoyuki, Inagaki.
Organizer
第38回日本神経科学会大会
Place of Presentation
神戸国際展示場(兵庫県神戸市)
Year and Date
2015-07-28
Related Report
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