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Regulation of neural development by acidic sugar chains via modification of morphogen activity

Research Project

Project/Area Number 26290027
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

IKENAKA Kazuhiro  生理学研究所, 分子細胞生理研究領域, 教授 (00144527)

Research Collaborator YOSHIMURA Takeshi  大阪大学, 大学院連合小児発達学研究科, 助教 (60402567)
ISHINO Yugo  
HASHIMOTO Hirokazu  
JIANG Wen  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords糖鎖 / 神経科学 / 生理学 / 脳・神経 / 発生分化
Outline of Final Research Achievements

Sulf1/2 are endosulfatases that remove sulfate from heparan sulfate. We found that Sulf2 modulates the cell fate change from motor neurons to oligodendrocyte precursor cells (MN-to-OPC) by regulating Shh signaling in the mouse ventral spinal cord in coordination with Sulf1. Sulf mRNAs colocalized with Shh mRNA and gradually expanded dorsally from E10.5 to E12.5, following strong Patched1 signals (inducec by Shh). In the spinal cord of Sulf1 or 2 knockout (KO) mice, expression patterns of Shh and Patched1 differed from that of wild type mice. Moreover, the position of the ventral domains was shifted ventrally, MN generation prolonged, and OPC generation delayed at E12.5 in KO mice. These results demonstrated that in addition to Sulf1, Sulf2 also plays an important role in the MN-to-OPC fate change by regulating Shh signaling. Strong Shh signaling is induced when Shh is released by Sulf1/2, and this strong Shh input subsequently induces the dorsal expansion of Shh and Sulf1/2 expression.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(13 results)

All 2017 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results) Presentation (8 results) (of which Invited: 3 results)

  • [Journal Article] Sulfatase2 Modulates Fate Change from Motor Neurons to Oligodendrocyte Precursor Cells through Coordinated Regulation of Shh Signaling with Sulfatase1.2017

    • Author(s)
      Jiang W, Ishino Y, Hashimoto H, Keino-Masu K, Masu M, Uchimura K, Kadomatsu K, Yoshimur T, Ikenaka K
    • Journal Title

      Developmental Neuroscience

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Keratan Sulfate Regulates the Switch from Motor Neuron to Oligodendrocyte Generation During Development of the Mouse Spinal Cord.2016

    • Author(s)
      Hashimoto H, Ishino Y, Jiang W, Yoshimura T, Takeda-Uchimura Y, Uchimura K, Kadomatsu K, Ikenaka K.
    • Journal Title

      Neurochem Res

      Volume: 41 Pages: 450-462

    • DOI

      10.1007/s11064-016-1861-9

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 大脳皮質オリゴデンドロサイトの起源2015

    • Author(s)
      成瀬雅衣、池中一裕、等誠司
    • Journal Title

      生体の科学

      Volume: 66 Pages: 541-545

    • Related Report
      2015 Annual Research Report
  • [Journal Article] The Dorsovental Boundary of the Germinal Zone is a Specialized Niche for the Generation of Cortical Oligodendorocytes during a Restricted Temporal Window.2015

    • Author(s)
      Naruse M, Ishino Y, Kumar A, Ono K, Takebayashi H, Yamaguchi M, Ishizaki Y, Ikenaka K, Hitoshi S
    • Journal Title

      Cereb Cortex

      Volume: online June 23 Pages: 1-11

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oligodendrocyte generation during mouse development.2015

    • Author(s)
      Takebayashi H, Ikenaka K
    • Journal Title

      Glia

      Volume: 63(8) Pages: 1350-1356

    • DOI

      10.1002/glia.22863

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] GlcNAc6ST-1はN結合型糖鎖の硫酸化を介して末梢神経系の髄鞘形成を制御する2015

    • Author(s)
      吉村武、林明子、内村健治、門松健治、矢木宏和、加藤晃一、馬場広子、池中一裕
    • Organizer
      第34回日本糖質学会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2015-07-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] 胎生期脊髄の発生におけるプロテオグリカンネットワークの役割2015

    • Author(s)
      橋本弘和、江文、Jinwoong Bok、池中一裕
    • Organizer
      第34回日本糖質学会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2015-07-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] 胎生期脊髄の発生におけるプロテオグリカンネットワークの役割2015

    • Author(s)
      橋本弘和、江文、Jinwoong Bok、池中一裕
    • Organizer
      第79回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      信州大学(長野県松本市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] オリゴデンドロサイト発生におけるプロテオグリカンの関与2014

    • Author(s)
      池中一裕
    • Organizer
      金沢大学薬学シンポジウム2014~グリアワールドへのいざない~
    • Place of Presentation
      金沢大学角間キャンパス(石川県金沢市)
    • Year and Date
      2014-12-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Regulation of oligodendrocyte development by proteoglycans2014

    • Author(s)
      池中一裕
    • Organizer
      International Conference on Frontiers in Comparative Endocrinology and Neurobiology-2014
    • Place of Presentation
      ハイデラ大学(インド ハイデラバード)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Regulation of oligodendrocyte development by proteoglycans2014

    • Author(s)
      池中一裕
    • Organizer
      First Meeting of the DFG Priority Programe 1757
    • Place of Presentation
      ハインリッヒハイネ大学(ドイツ デュッセルドルフ)
    • Year and Date
      2014-10-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ケラタン硫酸によるShhシグナリングを介した胎生期脊髄の発生制御2014

    • Author(s)
      橋本弘和、石野雄吾、江文、吉村武、内村佳子、内村健治、、門松健治、池中一裕
    • Organizer
      第33回日本糖質学会
    • Place of Presentation
      名古屋大学豊田講堂(愛知県名古屋市)
    • Year and Date
      2014-08-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Sulfatase KOマウスにおける胎生期脊髄オリゴデンドロサイトの発生異常2014

    • Author(s)
      江文、石野雄吾、橋本弘和、枡和子、枡正幸、池中一裕
    • Organizer
      第33回日本糖質学会
    • Place of Presentation
      名古屋大学豊田講堂(愛知県名古屋市)
    • Year and Date
      2014-08-10
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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