Generations of mice for imaging of cardiovascular system
Project/Area Number |
26290030
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory animal science
|
Research Institution | Shiga University of Medical Science |
Principal Investigator |
Ema Masatsugu 滋賀医科大学, 動物生命科学研究センター, 教授 (60359578)
|
Co-Investigator(Kenkyū-buntansha) |
三輪 佳宏 筑波大学, 医学医療系, 講師 (70263845)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
Keywords | 血管内皮細胞 / 腫瘍 / 発生 / 血管新生 / 動物 |
Outline of Final Research Achievements |
We generated a novel transgenic (Tg) mouse that expresses a bioluminescent reporter protein, Nano-lantern, under the control of Fetal liver kinase 1 (Flk1). Flk1-Nano-lantern BAC Tg mice recapitulated endogenous Flk1 expression in endothelial cells and lymphatic endothelial cells during development and tumour growth. Importantly, bioluminescence imaging of endothelial cells from the aortic rings of Flk1-Nano-lantern BAC Tg mice enabled us to observe endothelial sprouting for 18 hr without any detectable phototoxicity. Furthermore, Flk1-Nano-lantern BAC Tg mice achieved time-lapse luminescence imaging of tumour angiogenesis in freely moving mice with implanted tumours. We also Lrrc33 KO mice and demonstrated that Lrrc33 is important for the function of tumour endothelial cells.
|
Report
(4 results)
Research Products
(7 results)
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[Journal Article] Fluorescence and Bioluminescence Imaging of Angiogenesis in Flk1-Nano-lantern Transgenic Mice.2017
Author(s)
Matsushita J, Inagaki S, Nishie T, Sakasai T, Tanaka J, Watanabe C, Mizutani KI, Miwa Y, Matsumoto K, Takara K, Naito H, Kidoya H, Takakura N, Nagai T, Takahashi S, Ema M.
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Journal Title
Sci Rep.
Volume: 7
Issue: 1
Pages: 46597-46597
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Arf6 regulates tumour angiogenesis and growth through HGF-induced endothelial β1 integrin recycling.2015
Author(s)
Hongu T, Funakoshi Y, Fukuhara S, Suzuki T, Sakimoto S, Takakura N, Ema M, Takahashi S, Itoh S, Kato M, Hasegawa H, Mochizuki N, Kanaho Y.
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Journal Title
Nat. Commun.
Volume: 6
Issue: 1
Pages: 7925-7925
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Osteogenic capillaries orchestrate growth plate-independent ossification of the malleus2015
Author(s)
Matsuo K, Kuroda Y, Nango N, Shimoda K, Kubota Y, Ema M, Bakiri L, Wagner EF, Takeda Y, Yashiro W, Momose A
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Journal Title
Development
Volume: 142
Pages: 3912-3920
Related Report
Peer Reviewed
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[Presentation] Fluorescence and Bioluminescence Imaging of Angiogenesis in Flk1-Nano-lantern Transgenic Mice2016
Author(s)
M Ema, S Inagaki, T Nishie, T Sakasai, J Tanaka, Y Miwa, K Matsumoto, K Takara, H Naito, H Kidoya, N Takakura, T Nagai, S Takahashi, J Matsushita
Organizer
血管生物医学会
Place of Presentation
長崎
Year and Date
2016-12-08
Related Report
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