Induction of human allergic responses in 2nd generation NOG mice
Project/Area Number |
26290034
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory animal science
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Research Institution | Central Institute for Experimental Animals |
Principal Investigator |
TAKAHASHI TAKESHI 公益財団法人実験動物中央研究所, その他部局等, 室長 (80335215)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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Keywords | ヒト化マウス / アレルギー / 獲得免疫 / T細胞 / クラススイッチ / 病態モデル / 疾患モデル |
Outline of Final Research Achievements |
We extensively gene-modified immunodeficient NOG mice and 15 different sub-strains (2nd generation NOG mice) were generated. The hematopoiesis and immune functions were investigated after transplantation of human hematopoietic stem cells. For example, NOG-pRORgt-gc transgenic (Tg) mice showed enhanced human IgG production. NOG-human (h)TLSP-IL-13 Tg showed human IgE production. And 3 different NOG-HLA-A Tg and 2 different NOG-HLA-DR Tg mice were generated. We also demonstrated that human food allergy symptoms could be recapitulated in humanized NOG-hGM-CSF/IL-3 Tg mice with multiple lineages of human hematopoietic cells. Lactoglobulin specific IgE recombinant antibody was given to the humanized NOG-hGM-CSF/IL-3 and subsequently they were fed with Lactoglobulin proteins. The mice showed anaphylaxis manifested by the reduction of body temperature.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Sodium taurocholate cotransporting polypeptide inhibition efficiently blocks hepatitis B virus spread in mice with a humanized liver2016
Author(s)
Tasuku Nakabori, Hayato Hikita, Kazuhiro Murai, Yasutoshi Nozaki, Yugo Kai, Yuki Makino, Yoshinobu Saito, Satoshi Tanaka, Hiroshi Wada, Hidetoshi Eguchi, Takeshi Takahashi, Hiroshi Suemizu, Ryotaro Sakamori, Naoki Hiramatsu, Tomohide Tatsumi, and Tetsuo Takehara
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 27782-27782
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing.2016
Author(s)
Sato K, Oiwa R, Kumita W, Henry R, Sakuma T, Ito R, Nozu R, Inoue T, Katano I, Sato K, Okahara N, Okahara J, Shimizu Y, Yamamoto M, Hanazawa K, Kawakami T, Kametani Y, Suzuki R, Takahashi T, Weinstein EJ, Yamamoto T, Sakakibara Y, Habu S, Hata J, Okano H, Sasaki E.
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Journal Title
Cell Stem Cell
Volume: 19
Issue: 1
Pages: 127-138
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Predominant Development of Mature and Functional Human NK Cells in a Novel Human IL-2-Producing Transgenic NOG Mouse2015
Author(s)
Katano, I. Takahashi, T. Ito, R. Kamisako, T. Mizusawa, T. Ka, Y. Ogura, T. Suemizu, H. Kawakami, Y. Ito, M.
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Journal Title
J Immunol
Volume: 194
Issue: 7
Pages: 3513-3525
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The Hepatitis B Virus Genotype Affects the Persistence of Viral Replication in Immunodeficient NOG Mice.2015
Author(s)
Yokoyama Y, Miyagi T, Hikita H, Yoshioka T, Mukai K, Nawa T, Sakamori R, Ohkawa K, Hiramatsu N, Takahashi T, Suemizu H, Ryo A, Tatsumi T, Takehara T.
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Journal Title
PLoS One.
Volume: 10(12)
Issue: 12
Pages: e0144775-e0144775
DOI
Related Report
Peer Reviewed / Open Access
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