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Analysis of mechanisms to avoid stoichiometry imbalance

Research Project

Project/Area Number 26290069
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field System genome science
Research InstitutionOkayama University

Principal Investigator

Moriya Hisao  岡山大学, 異分野融合先端研究コア, 准教授 (60500808)

Co-Investigator(Kenkyū-buntansha) 牧野 能士  東北大学, 生命科学研究科, 准教授 (20443442)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords化学量不均衡 / 出芽酵母 / 遺伝子発現 / タンパク質分解 / ロバストネス / ゲノム / 発現制御 / タンパク質 / 微生物 / 酵母 / ユビキチンープロテアソーム系
Outline of Final Research Achievements

About one third of proteins function as protein complexes. Imbalances in the stoichiometry of subunits constituting protein complexes sometimes cause harmful effects on cellular functions. In this study, using budding yeast, we revealed a mechanism to avoid stoichiometry imbalances in protein complexes. We identified several proteins whose expressions were not increased as expected when their gene copy numbers were artificially increased. The buffering of protein expression was performed through active degradation of excess proteins by the ubiquitin-proteasome system.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (30 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (18 results) (of which Int'l Joint Research: 6 results,  Invited: 7 results) Remarks (5 results)

  • [Int'l Joint Research] University of California, Berkeley(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Toronto(カナダ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Post-translational dosage compensation buffers genetic perturbations to stoichiometry of protein complexes2017

    • Author(s)
      Koji Ishikawa, Koji Makanae, Shintaro Iwasaki, Nicholas T. Ingolia, Hisao Moriya
    • Journal Title

      PLoS Genetics

      Volume: 13(1) Issue: 1 Pages: e1006554-e1006554

    • DOI

      10.1371/journal.pgen.1006554

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Cellular growth defects triggered by an overload of protein localization processes2016

    • Author(s)
      Kintaka R, Makanae K, Moriya H.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 31774-31774

    • DOI

      10.1038/srep31774

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Quantitative nature of overexpression experiments.2016

    • Author(s)
      Moriya H
    • Journal Title

      Mol Biol Cell

      Volume: 26 Issue: 22 Pages: 3932-3939

    • DOI

      10.1091/mbc.e15-07-0512

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aneuploid proliferation defects in yeast are not driven by copy number changes of a few dosage-sensitive genes2015

    • Author(s)
      Bonney ME, Moriya H, Amon A
    • Journal Title

      Genes Dev.

      Volume: 29 Issue: 9 Pages: 898-903

    • DOI

      10.1101/gad.261743.115

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Small Toxic Protein Encoded on Chromosome VII of Saccharomyces cerevisiae2015

    • Author(s)
      Makanae K, Kintaka R, Ishikawa K, Moriya H
    • Journal Title

      PLoS One

      Volume: 10 Issue: 3 Pages: e0120678-e0120678

    • DOI

      10.1371/journal.pone.0120678

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Consequences of protein overexpression2016

    • Author(s)
      Hisao Moriya
    • Organizer
      The 26th Hot Spring Harbor International Symposium
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2016-11-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Consequences of protein overexpression2016

    • Author(s)
      Hisao Moriya, Reiko Kintaka, Charles Boone, Koji Ishikawa, Yuichi Eguchi
    • Organizer
      14th International Congress on Yeasts
    • Place of Presentation
      兵庫県淡路市
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression limits of glycolytic enzymes in yeast2016

    • Author(s)
      Yuichi Eguchi, Koji Makanae, Yoshimi Hori, Tomohisa Hasunuma, Hisao Moriya
    • Organizer
      14th International Congress on Yeasts
    • Place of Presentation
      兵庫県淡路市
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Overloads of protein localization process cause cellular growth defects2016

    • Author(s)
      Reiko Kintaka, Koji Makanae, Hisao Moriya
    • Organizer
      14th International Congress on Yeasts
    • Place of Presentation
      兵庫県淡路市
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Perturbed stoichiometry of complex subunits is circumvented by protein-level dosage compensation2016

    • Author(s)
      Koji Ishikawa, Koji Makanae, Hisao Moriya
    • Organizer
      The 9th Asian Young Res. Conf. on Comput. and Omics Biol.
    • Place of Presentation
      シンガポール
    • Year and Date
      2016-01-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] タンパク質の過剰発現が及ぼす影響とそれに対する細胞のストラテジー2015

    • Author(s)
      守屋 央朗、石川 浩史、江口 優一、金高 令子、蒔苗 浩司
    • Organizer
      BMB2015
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 遺伝子発現系への摂動をタンパク質レベルで緩衝する新たな分子機構の解析2015

    • Author(s)
      石川 浩史、蒔苗 浩司、守屋 央朗
    • Organizer
      BMB2015
    • Place of Presentation
      神戸市
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Robust gene expression under genetic perturbation: New mechanism maintaining stoichiometry of protein complex2015

    • Author(s)
      Koji Ishikawa, Koji Makanae, Hisao Moriya
    • Organizer
      The 16th International Conference on Systems Biology
    • Place of Presentation
      シンガポール
    • Year and Date
      2015-11-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 遺伝子発現の摂動に対してタンパク質発現量を調整する 新たな分子機構の解析2015

    • Author(s)
      石川浩史、蒔苗浩司、守屋央朗
    • Organizer
      酵母遺伝学フォーラム第48回報告会
    • Place of Presentation
      東広島市
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞内でタンパク質発現量の限界を決める要因はなにか?2015

    • Author(s)
      守屋央朗
    • Organizer
      第5回合成生物工学シンポジウム
    • Place of Presentation
      神戸市
    • Year and Date
      2015-08-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞の持つタンパク質発現のキャパシティーを測る2015

    • Author(s)
      守屋央朗
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山県・岡山市
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Systematic identification of proteins whose expressions are compensated upon expression perturbation in Saccharomyces cerevisiae2015

    • Author(s)
      Koji Ishikawa
    • Organizer
      Cold Spring Harbor Meeting Systems Biology: Global Regulation of Gene Expression 2015
    • Place of Presentation
      Puerto Rico, USA
    • Year and Date
      2015-01-28 – 2015-02-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 出芽酵母における遺伝子発現量の乱れを補正する機構のシステマティックな解析2014

    • Author(s)
      石川浩史
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      神奈川県・横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] タンパク質発現量の限界を決める要素は何か?2014

    • Author(s)
      守屋央朗
    • Organizer
      「細胞を創る」研究会7.0
    • Place of Presentation
      東京都・文京区
    • Year and Date
      2014-11-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 細胞システムのロバストネスを測る2014

    • Author(s)
      守屋央朗
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都府・京都市
    • Year and Date
      2014-10-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 細胞システムのロバストネスを測る2014

    • Author(s)
      守屋央朗
    • Organizer
      「制御・情報理論による生物システムのロバストネス解析と設計」第1回公演
    • Place of Presentation
      東京都・文京区
    • Year and Date
      2014-09-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 遺伝子発現量の乱れを補正する機構のシステマティックな解析2014

    • Author(s)
      石川浩史
    • Organizer
      酵母遺伝学フォーラム第47回研究報告会
    • Place of Presentation
      東京都・文京区
    • Year and Date
      2014-09-01 – 2014-09-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] タンパク質の発現量の限界を測る。2014

    • Author(s)
      守屋央朗
    • Organizer
      日本プロテオーム学会2014年会
    • Place of Presentation
      茨城県・つくば市
    • Year and Date
      2014-07-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Remarks] システム細胞学研究室

    • URL

      http://tenure5.vbl.okayama-u.ac.jp/HMlab/

    • Related Report
      2016 Annual Research Report
  • [Remarks] HM's website

    • URL

      http://tenure5.vbl.okayama-u.ac.jp/~hisaom/HMwiki/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 岡山大学異分野融合先端研究コア・システム細胞学研究室

    • URL

      http://tenure5.vbl.okayama-u.ac.jp/HMlab/

    • Related Report
      2015 Annual Research Report
  • [Remarks] ブログ:酵母とシステム生物学

    • URL

      http://tenure5.vbl.okayama-u.ac.jp/HM_blog/

    • Related Report
      2015 Annual Research Report
  • [Remarks] HM's webpage

    • URL

      http://tenure5.vbl.okayama-u.ac.jp/~hisaom/HMwiki/

    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2022-02-16  

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