Analysis of mechanisms to avoid stoichiometry imbalance
| Project/Area Number |
26290069
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
System genome science
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| Research Institution | Okayama University |
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Principal Investigator |
Moriya Hisao 岡山大学, 異分野融合先端研究コア, 准教授 (60500808)
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| Co-Investigator(Kenkyū-buntansha) |
牧野 能士 東北大学, 生命科学研究科, 准教授 (20443442)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 化学量不均衡 / 出芽酵母 / 遺伝子発現 / タンパク質分解 / ロバストネス / ゲノム / 発現制御 / タンパク質 / 微生物 / 酵母 / ユビキチンープロテアソーム系 |
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| Outline of Final Research Achievements |
About one third of proteins function as protein complexes. Imbalances in the stoichiometry of subunits constituting protein complexes sometimes cause harmful effects on cellular functions. In this study, using budding yeast, we revealed a mechanism to avoid stoichiometry imbalances in protein complexes. We identified several proteins whose expressions were not increased as expected when their gene copy numbers were artificially increased. The buffering of protein expression was performed through active degradation of excess proteins by the ubiquitin-proteasome system.
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Report
(4 results)
Research Products
(30 results)
