Regulation mechanisms for tissue-specific post-transcriptional pre-mRNA processing.

• Project/Area Number: 26291003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KUROYANAGI Hidehito 東京医科歯科大学, 難治疾患研究所, 准教授 (30323702)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: 遺伝子発現 / 新生RNA / 線虫 / 組織特異性 / 転写後プロセシング / mRNA / ChIP-seq / 代謝標識 / RNAseq / mRNA前駆体

Outline of Final Research Achievements

We revealed binding regions of LST-3 genome wide and mechanisms for splicing regulation by LST-3. We have analyzed tissue-specific alternative splicing patterns of the lev-11 gene, encoding tropomyosin, in C. elegans. We published a review article on alternative splicing regulation in C. elegans. We tried TU-tagging to label nascent RNAs in tissue-specific manners in living worms.

Research Products

• [Int'l Joint Research] Emory University(米国)
• [Int'l Joint Research] University of California, Los Angeles/Emory University(米国)
• [Journal Article] Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans (2018)
  • Author(s): Barnes Dawn E.、Watabe Eichi、Ono Kanako、Kwak Euiyoung、Kuroyanagi Hidehito、Ono Shoichiro
  • Journal Title: Molecular Biology of the Cell Volume: 29 Issue: 9 Pages: 1075-1088
  • DOI: 10.1091/mbc.e17-03-0152
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An emerging model organism Caenorhabditis elegans for alternative pre-mRNA processing in vivo (2017)
  • Author(s): Shotaro Wani & Hidehito Kuroyanagi
  • Journal Title: Wiley Interdisciplinary Reviews: RNA Volume: 印刷中 Issue: 6
  • DOI: 10.1002/wrna.1428
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor. (2016)
  • Author(s): Masahiro Tomioka, Yasuki Naito, Hidehito KUROYANAGI, and Yuichi Iino.
  • Journal Title: Nature Communications Volume: - Issue: 1 Pages: 11645-11645
  • DOI: 10.1038/ncomms11645
  • NAID: 120006348540
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evolutionarily conserved autoregulation of alternative pre-mRNA splicing by ribosomal protein L10a. (2016)
  • Author(s): Satomi Takei, Marina Togo-Ohno, Yutaka Suzuki and Hidehito Kuroyanagi
  • Journal Title: Nucleic Acids Research Volume: - Issue: 12 Pages: 5585-5596
  • DOI: 10.1093/nar/gkw152
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] RBFOX and SUP-12 sandwich a guanine base to form a stable complex and regulate tissue-specific splicing. (2014)
  • Author(s): Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Guntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, and Muto Y.
  • Journal Title: Nat. Struct. Mol. Biol. Volume: 21(9) Issue: 9 Pages: 778-86
  • DOI: 10.1038/nsmb.2870
  • Peer Reviewed / Open Access
• [Journal Article] 組織特異的にスプライシング反応を制御するRBFOXファミリーRNA結合タンパク質およびSUP-12によるRNAの協働的な認識の機構. (2014)
  • Author(s): 桑迫香奈子、高橋真梨、黒柳秀人、武藤 裕.
  • Journal Title: ライフサイエンス 新着論文レビュー Volume: archives Pages: 9218-9218
• [Presentation] Genome-Wide Kinetic Analysis of Pre-mRNA Processing in Living Animals (2018)
  • Author(s): 渡部栄地
  • Organizer: 東京地区線虫勉強会
• [Presentation] C. elegans代謝関連遺伝子のスプライシング制御における食餌の影響 (2018)
  • Author(s): 和仁翔太郎
  • Organizer: 東京地区線虫勉強会
• [Presentation] 個体レベルで行うmRNA前駆体プロセシングの動態の解析 (2017)
  • Author(s): 渡部栄地、黒柳秀人
  • Organizer: 第19回日本RNA学会年会
• [Presentation] C. elegans代謝関連遺伝子のスプライシング制御における食餌の影響 (2017)
  • Author(s): 和仁翔太郎、黒柳秀人
  • Organizer: 第19回日本RNA学会年会
• [Presentation] Genome-wide kinetic analysis of pre-mRNA processing in living animals (2017)
  • Author(s): Eichi Watabe, Hidehito Kuroyanagi
  • Organizer: CSHL Meeting on 40 YEARS OF mRNA SPLICING: FROM DISCOVERY TO THERAPEUTICS
• [Presentation] 個体レベルで行う mRNA前駆体プロセシングの動態の解析 (2017)
  • Author(s): 渡部栄地、黒柳秀人
  • Organizer: ConBio2017（2017年度生命科学系学会合同年次大会）
• [Presentation] 生体内mRNA前駆体プロセシングの動態の解析 (2017)
  • Author(s): 渡部栄地
  • Organizer: 東京地区線虫勉強会
  • Place of Presentation: 東京大学
• [Presentation] Spatial and temporal analysis of nascent pre-mRNAs in living animals. (2016)
  • Author(s): Eichi Watabe and Hidehito Kuroyanagi
  • Organizer: RNA 2016: The 21st Annual Meeting of the RNA Society（第18回日本RNA学会年会共催）
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] 個体レベルで行う組織特異的転写後プロセシングの空間的・時間的動態の解析 (2016)
  • Author(s): 渡部栄地、黒柳秀人
  • Organizer: 冬の若手ワークショップ201６＠山中湖
  • Place of Presentation: 山梨県南都留郡山中湖村
  • Year and Date: 2016-02-04
• [Presentation] "Non-Coding mRNAs" from Protein-Coding Genes through Alternative Pre-mRNA Splicing. (2016)
  • Author(s): Hidehito Kuroyanagi
  • Organizer: 19th Tokyo RNA Club
  • Place of Presentation: 東京大学
  • Int'l Joint Research / Invited
• [Presentation] mRNA前駆体の転写とスプライシングを自己制御する因子の解析 (2015)
  • Author(s): 黒柳秀人
  • Organizer: 東京地区線虫勉強会
  • Place of Presentation: 東京大学浅野キャンパス
  • Year and Date: 2015-11-07
• [Presentation] RBFOX and SUP-12 cooperatively regulate muscle-specific alternative splicing to determine ligand-binding specificity of FGF receptors in C. elegans (2014)
  • Author(s): Hidehito Kuroyanagi
  • Organizer: 理研シンポジウム「Noncoding RNA Regulation」
  • Place of Presentation: 理化学研究所 和光キャンパス 鈴木梅太郎ホール
  • Year and Date: 2014-10-01
  • Invited
• [Presentation] 選択的スプライシング制御因子ASD-1とSUP-12による協働的なRNA 認識の構造基盤 (2014)
  • Author(s): 桑迫香奈子，高橋真梨，雲財悟，津田健吾，吉川征子，何発虎，小林直弘，Peter Güntert，白水美香子，伊藤拓宏，田仲昭子，横山茂之，萩原正敏，黒柳秀人，武藤裕.
  • Organizer: 第16回日本RNA 学会年会
  • Place of Presentation: ウィンクあいち
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] Cooperative regulation of tissue-specific alternative splicing by multiple splicing factors determines ligand-binding specificity of FGF receptors. (2014)
  • Author(s): Hidehito Kuroyanagi.
  • Organizer: The 9th International Symposium of the Institute Network
  • Place of Presentation: 大阪大学
  • Year and Date: 2014-06-19 – 2014-06-20
  • Invited
• [Book] 遺伝子発現制御機構 (2017)
  • Author(s): 田村隆明、浦聖惠（編）
  • Total Pages: 264
  • Publisher: 東京化学同人
  • ISBN: 9784807909179
• [Book] 基礎分子生物学II:遺伝子発現制御機構ークロマチン，転写制御，エピジェネティクスー(仮称) (2017)
  • Author(s): 田村隆明・浦聖恵（編） 黒柳秀人を含むのべ38名（著）
  • Publisher: 東京化学同人
• [Remarks] 東京医科歯科大学 難治疾患研究所 フロンティア研究室 遺伝子発現制御学
  • URL: http://www.tmd.ac.jp/end/
• [Remarks] 新学術領域研究「ノンコーディングRNAネオタクソノミ」研究成果紹介
  • URL: https://ncrna.jp/blog/item/370-rouge
• [Remarks] みらいぶプラス
  • URL: https://www.milive-plus.net/newleader/049/
• [Remarks] 東京医科歯科大学難治疾患研究所遺伝子発現制御学 研究成果の紹介
  • URL: http://www.tmd.ac.jp/end/research/
• [Remarks] UTokyo Research
  • URL: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/production-of-a-molecule-for-memory-of-salt-concentration.html
• [Remarks] 東京医科歯科大学難治疾患研究所フロンティア研究室遺伝子発現制御学
  • URL: http://www.tmd.ac.jp/end/
• [Remarks] 東京医科歯科大学難治疾患研究所
  • URL: http://www.tmd.ac.jp/mri/press/