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High-dimensional structure of chromatin and transcription regulation

Research Project

Project/Area Number 26291006
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Molecular biology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Ishii Shunsuke  国立研究開発法人理化学研究所, 石井分子遺伝学研究室, 上席研究員 (00124785)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsヘテロクロマチン / ATF7 / ストレス / エピゲノム変化 / クロマチン / 高次構造 / 転写制御 / エピゲノム / ユークロマチン / 環境因子 / 脂肪細胞 / 代謝系遺伝子
Outline of Final Research Achievements

High-dimensional structure of chromatin has an important role for transcription regulation. Chromatin consists of the heterochromatin, which is localized close to the centromere and telomere, and the euchromatin. We found that ATF7 contributes to heterochromatin formation by recruiting histone H3K9 methyltransferases, and that the stress-induced phosphorylation of ATF7 by p38 causes the disruption of heterochromatin and transcriptional induction. This effect was maintained for long period. Thus, it was shown that ATF7 plays an important role for regulation of the high-dimensional structure of chromatin.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(20 results)

All 2017 2016 2015 2014

All Journal Article (10 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 10 results,  Acknowledgement Compliant: 10 results,  Open Access: 8 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results)

  • [Journal Article] Structural analyses of the nucleosome complexes with human testis-specific histone variants, hTh2a and hTh2b.2017

    • Author(s)
      Padavattan S et al.
    • Journal Title

      Biophys Chem

      Volume: 221 Pages: 41-48

    • DOI

      10.1016/j.bpc.2016.11.013

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] ATF7 ablation prevents diet-induced obesity and insulin resistance.2016

    • Author(s)
      Liu Y et al.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 478 Pages: 696-702

    • DOI

      10.1016/j.bbrc.2016.08.009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] In utero TNF-α treatment induces telomere shortening in young adult mice in an ATF7-dependent manner2016

    • Author(s)
      B. Liu, T Maekawa, B. Chatton, S. Ishii
    • Journal Title

      FEBS Open Bio.

      Volume: 6 Pages: 56-63

    • DOI

      10.1002/2211-5463.12006

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Two histone variants TH2A and TH2B enhance human iPS cell generation.2016

    • Author(s)
      Huynh ML
    • Journal Title

      Stem Cells Dev.

      Volume: 25 Pages: 251-258

    • DOI

      10.1089/scd.2015.0264

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The transcription factor ATF7 mediates lipopolysaccharide-induced epigenetic changes in macrophages involved in innate immunological memory.2015

    • Author(s)
      Yoshida K, Maekawa T, Zhu Y, Renard-Guillet C, Ishibashi K,Chatton B, Inoue K, Uchiyama T, Yamada T, Ohno N, Shirahige K, Okada-Hatakeyama M, Ishii S.
    • Journal Title

      Nat. Immunol.

      Volume: 16(10) Pages: 1034-1043

    • DOI

      10.1038/ni.3257

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Structural and functional analyses of nucleosome complexes with mouse histone variants TH2a and TH2b, involved in reprogramming.2015

    • Author(s)
      Padavattan S et al.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 464 Pages: 929-935

    • DOI

      10.1016/j.bbrc.2015.07.070

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Combined behavioral studies and in vivo imaging of inflammatory response and expression of mGlu5 receptors in schnurri-2 knockout mice.2015

    • Author(s)
      Choi JK, Zhu A, Jenkins BG, Hattori S, Kil KE, Takagi T, Ishii S, Miyakawa T, Brownell AL.
    • Journal Title

      Neurosci. Lett.

      Volume: 609 Pages: 159-164

    • DOI

      10.1016/j.neulet.2015.10.037

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Microarray expression analysis of genes involved in innate immune memory in peritoneal macrophages.2015

    • Author(s)
      Yoshida K, Renard-Guillet C, Inoue K, Shirahige K, Okada-Hatakeyama M, Ishii S.
    • Journal Title

      Genomics Data

      Volume: 7 Pages: 90-1

    • DOI

      10.1016/j.gdata.2015.11.028

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] T396I Mutation of Mouse Sufu Reduces the Stability and Activity of Gli3 Repressor2015

    • Author(s)
      Makino S, Zhulyn O, Mo R, Puviindran V, Zhang X, Murata T, Fukumura R, Ishitsuka Y, Kotaki H, Matsumaru D, Ishii S, Hui C.C, Gondo Y
    • Journal Title

      PLoS ONE

      Volume: 10

    • DOI

      10.1371/journal.pone.0119455

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Disruption of Th2a and Th2b genes causes defects in spermatogenesis.2015

    • Author(s)
      Shinagawa, T. et al.
    • Journal Title

      Development

      Volume: 142 Pages: 1287-1292

    • DOI

      10.1242/dev.121830

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Innate immune memory in macrophages via ATF7-dependent epigenetic changes.2017

    • Author(s)
      Ishii S
    • Organizer
      Conference on Innate Immune Memory
    • Place of Presentation
      ケンブリッジ、英国
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis for trans-generational inheritance of epigenetic change induced by pathogen infection via ATF7.2017

    • Author(s)
      Yohida K, Ishii S
    • Organizer
      Conference on Innate Immune Memory
    • Place of Presentation
      ケンブリッジ、英国
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 精子を介したエピゲノム情報の伝達2017

    • Author(s)
      石井俊輔
    • Organizer
      ワークショップ「染色体研究の最前線」
    • Place of Presentation
      大阪大学(大阪府、吹田市)
    • Year and Date
      2017-01-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 環境要因によるエピゲノム変化の世代を超えた遺伝2016

    • Author(s)
      石井俊輔
    • Organizer
      第10回日本エピジェネティクス研究会年会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府、豊中市)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 低タンパク質餌によるエピゲノム変化の世代を超えた遺伝2016

    • Author(s)
      吉田圭介、石井俊輔
    • Organizer
      第10回日本エピジェネティクス研究会年会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府、豊中市)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] Epigenome changes induced by environmental factors and their memory.2015

    • Author(s)
      石井俊輔
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] TNF-α treatment in fathers programs telomere shortening in mouse offspring.2015

    • Author(s)
      Binbin Liu et al.
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 転写因子ATF-7を介したストレスによるテロメアの長さの制御2014

    • Author(s)
      前川利男、Binbin Liu、吉田圭介、仲村賢一、田久保海誉、石井俊輔
    • Organizer
      第37回に本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 父親への拘束ストレスによるエピゲノム変化と遺伝の解析2014

    • Author(s)
      成耆鉉、石井俊輔
    • Organizer
      第37回に本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 精子形成におけるヒストンバリアントTH2AとTH2Bの役割2014

    • Author(s)
      品川敏恵、Huynh Linh My、高木豪、塚本大輔、都丸千夏、野口純子、石井俊輔
    • Organizer
      第37回に本分子生物学会年会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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