| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
V-ATPases function as ATP-dependent ion pumps, the hydrophilic V1 portion is known as rotary motor in which a central axis DF complex rotates inside a hexagonally arranged catalytic A3B3 complex using ATP hydrolysis energy. We previously succeeded in obtaining the crystal structures of the A3B3 complex which is a three-fold assembly of the identical A1B1 pair, but showed asymmetrical hexamer ring structure. In this study, we elucidated the crystal structures and biochemical properties of the A-subunit, B-subunit, A1B1 complex, and A3B3 mutant. Based on these and previous findings, we propose a molecular mechanism model of the structural formation and functional regulation of the V1 rotary motor.
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