| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
To perform the rapid mixing in the NMR magnet and to observe the folding or aggregation reaction of a protein in the atomic resolution, we devised an apparatus for the mixing of two solutions. Denatured protein solution goes through a first tubing and refolding buffer second tubing, and both tubing are connected to a carefully designed mixer in a NMR tube in the magnet. Waste solution is removed from the third tubing thereby enabling the observation in a continuous flow in the NMR tube. We reformed the apparatus used for in cell NMR using the glass material with homogeneous magnetic susceptibility with the aqueous solution. Thus we could remove the false peaks originated from the inhomogeneous magnetic susceptibility that was observed in the previous version of the apparatus. Also we found the ‘A-state’ which corresponds to the branching point between the cellular form and the aggregate, and that the cellular form itself inhibits the aggregate formation uncompetitively.
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