| Project/Area Number |
26291012
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Miki Kunio 京都大学, 理学(系)研究科(研究院), 教授 (10116105)
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| Co-Investigator(Kenkyū-buntansha) |
渡部 聡 東北大学, 多元物質科学研究所, 助教 (50432357)
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| Co-Investigator(Renkei-kenkyūsha) |
ATOMI Haruyuki 京都大学, 大学院工学研究科, 教授 (90243047)
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| Research Collaborator |
NISHITANI Yuichi 京都大学, 大学院理学研究科, 特定研究員
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 成熟化タンパク質 / X線結晶構造解析 / 金属取り込み / Hypタンパク質 / 複合体構造 / 構造生物学 / Ｘ線結晶解析 / タンパク質 |
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| Outline of Final Research Achievements |
Six Hyp proteins (HypA, B, C, D, E, and F) are primarily involved in the maturation process of [NiFe] hydrogenases which catalyze reversible hydrogen production and consumption. These Hyp proteins incorporate a Ni/Fe cluster into [NiFe] hydrogenases. On the basis of our previous success in the structure determination of several Hyp proteins and their complexes, we have investigated structure and function of transient complexes such as HypAB complex, hydrogenase large subunit HyhL and Hyp protein complexes, and protease HybD. In addition, we have tried to identify a candidate for the Ni metallochaperone. These experimental results provided us information about the molecular mechanism of the [NiFe] hydrogenase maturation.
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