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Substrate dependent selective recruitment for the co-activators by the use of the structure dynamics of nuclear receptor

Research Project

Project/Area Number 26291015
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionHiroshima University

Principal Investigator

Tate Shin-ichi  広島大学, 理学(系)研究科(研究院), 教授 (20216998)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywordsnuclear receptor / co-activator / transient structure / NMR / structure dynamics / time resolution FRET / 核内受容体 / 低存在率構造 / 天然変性領域 / FRET / 共役因子リクルート / 安定同位体標識 / PPARg / 異方性核スピン効果
Outline of Final Research Achievements

We explored the mechanism of the substrate-dependent selective recruitment of the co-activator to PPARg, a type of nuclear receptor. We determined the solution structure of PPARg in the complex with agonist to demonstrate the relative orientation between H3 and H4 surrounding to the co-activator biding pocket was changed, which have been never found in the X-ray structures.
We analyzed the low-population structure of the fragments having the PPARg binding motifs, LxxLL, in SRC1, a co-activator. We revealed that two fragments had different low population structures at the site of LxxLL motif and the C-terminal parts following the binding motif. We also found the C-terminal low population structures are responsible for the co-activator binding to the PPARg. The dynamic structural properties of SRC1 should determine the substrate-dependent co-activator recruitment to the PPARg.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(35 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research Journal Article Presentation Remarks Patent(Industrial Property Rights)

  • [Int'l Joint Research] University of Lyon 1(France)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Ulsan Inst. Sci. & Tech.(Korea)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Academia Sinica/清華大学(台湾)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Lyon1(フランス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Exploring reaction pathways for peptidylprolyl-isomerase2017

    • Author(s)
      Fujisaki,H., Yonezawa,Y., Shiga,M., Maragliano,L., Tate,S.
    • Journal Title

      Biophys. J.

      Volume: 112

    • DOI

      10.1016/j.bpj.2016.11.2407

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] TALEタンパク質の超らせん運動とDNA配列認識2017

    • Author(s)
      楯 真一
    • Journal Title

      生物物理

      Volume: 57

    • NAID

      130005678985

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy2016

    • Author(s)
      Tochio,N., Umehara,K., Uewaki,J., Flechsig,H., Kondo,M., Dewa,T., Sakuma,T., Yamamoto,T., Saito,T., Togashi,T., Tate,S.
    • Journal Title

      Scientific Reports

      Volume: 6 Pages: 37887-37887

    • DOI

      10.1038/srep37887

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Sequential backbone resonance assignments of the E.coli dihydrofolate reductase Gly67Val mutant:folate complex2016

    • Author(s)
      Narayanan,S.P., Maeno,A., Wada,Y., Tate,S., Akasaka,K.
    • Journal Title

      J.Biomol.NMR Assign.

      Volume: 10 Pages: 125-129

    • DOI

      10.1007/s12104-015-9650-y

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Allosteric breakage to the hydrogen bond within the dual-histidine motif in the active site of human Pin1 PPIase2015

    • Author(s)
      Wang,J., Tochio,N., Kawasaki,R., Tamari,Y., Xu,N., Uewaki,J., Utsunomiya-Tate,N., Tate,S.
    • Journal Title

      Biochemistry

      Volume: 54 Pages: 5242-5253

    • DOI

      10.1021/acs.biochem.5b00606

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Coating the outer surface of glass nanopipette with chlorobenzene-terminated polysilioxane2015

    • Author(s)
      Takami,T., Ojiro,Y., Ogawa,S., Takaku,Y., Ogawa,Y., Saito,M., Matuoka,H., Tate,S.
    • Journal Title

      e-J.Surf.Sci. Nanotech

      Volume: 13 Pages: 79-84

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Coating the Outer Surface of Glass Nanopipette with Chlorobenzene-Terminated Polysiloxane2015

    • Author(s)
      T. Takami, Y. Ojiro, Y. Ogawa, S. Ogawa, Y. Takakuwa, M. Saito, H. Matsuoka, S. Tate
    • Journal Title

      e-J. Surf. Sci. Nanotech.

      Volume: 13 Issue: 0 Pages: 79-84

    • DOI

      10.1380/ejssnt.2015.79

    • NAID

      130004933837

    • ISSN
      1348-0391
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The C113D mutation in human Pin1 causes allosteric structural changes in the phosphate binding pocket of the PPIase domain through the tug of war in the dual-histidine motif2014

    • Author(s)
      Xu,N., Tochio,N., Wang,J., Tamari,Y., Uewaki,J., Utsunomiya-Tate,N., Igarashi,K., Shiraki,T, Kobayashi,N., Tate,S.
    • Journal Title

      Biochemistry

      Volume: 53 Pages: 5568-5578

    • DOI

      10.1021/bi5007817

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Inter-domain communication between the folded proteins tethered by the intrinsically disordered regions (IDRs)2017

    • Author(s)
      Tate,S.
    • Organizer
      Asia Pasific NMR 2017 (APNMR2017)
    • Place of Presentation
      Bangalore, India
    • Year and Date
      2017-02-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Inter-domain communication between the domains tethered by intrinsically disordered region (IDR)-intramolecular 'fly-casting' mechanism facilitating ligand recognition2016

    • Author(s)
      Tate,S.
    • Organizer
      The 7th Asia-Pacific Symposium on IDPs (AIDPs)
    • Place of Presentation
      Daejun, Korea
    • Year and Date
      2016-10-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ultra-sensitive regulation of the nucleosome binding of FACT, a chromatin remodeler, through multiple phosphorylation to its intrinsically disordered regions (IDRs)2016

    • Author(s)
      Tate,S.
    • Organizer
      the 14th Discovery on TARGET
    • Place of Presentation
      Boston, USA
    • Year and Date
      2016-09-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional regulation through the dynamic ensembles of protein structures mediated by intrinsically disordered regions (IDRs)2016

    • Author(s)
      Tate,S.
    • Organizer
      ICMRBS2016
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2016-08-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Protein structure and dynamics in different magnitudes and time domains for folded and intrinsically disordered proteins (IDPs)2016

    • Author(s)
      Tate,S.
    • Organizer
      UNIST invited lecture
    • Place of Presentation
      Ulsan, Korea
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] タンパク質構造の構造ダイナミクスと機能制御ー安定構造をもつタンパク質から天然変性タンパク質までを対象として2016

    • Author(s)
      楯 真一
    • Organizer
      日本学術振興会・構造生物169員会 研究会
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Structural dynamics and their functional implications of the intrinsically disordered regions (IDRs) in transcription regulatory proteins2015

    • Author(s)
      Shin-ichi Tate
    • Organizer
      Pacifichem2016
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Structural dynamics and functions of folded and intrinsically disordered proteins (IDPs)2015

    • Author(s)
      Shin-ichi Tate
    • Organizer
      Inst. Sciences Analytiques Seminar in Lyon1 Univ.
    • Place of Presentation
      Lyon, France
    • Year and Date
      2015-11-29
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Functional significance of the transient folding of intrinsically disordered proteins (IDPs)2015

    • Author(s)
      Shin-ichi Tate
    • Organizer
      第53回 日本生物物理学会年会
    • Place of Presentation
      金沢
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] How life can be understood by physics and mathematics2015

    • Author(s)
      Shin-ichi Tate
    • Organizer
      Inst. Mathematics in Univ. Science and Technology of China
    • Place of Presentation
      Shanghai, China
    • Year and Date
      2015-09-03
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Functional significance of the transient folding of intrinsically disordered proteins (IDPs)2015

    • Author(s)
      Shin-ichi Tate
    • Organizer
      第15回 日本蛋白質科学会年会
    • Place of Presentation
      徳島
    • Year and Date
      2015-06-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Functional significance of the transient folding of intrinsically disordered proteins (IDPs) revealed by combinatorial approaches including high-seed AFM, SAXS, molecular dynamics and NMR2015

    • Author(s)
      Shin-ichi Tate
    • Organizer
      Gordon Research Seminar
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2015-06-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional significance of the transient folding of intrinsically disordered proteins (IDPs) revealed by the combinatorial approaches2015

    • Author(s)
      Shin-ichi Tate
    • Organizer
      Academia Sinica Inviting Seminar
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2015-04-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 酵素活性部位水素結合ネットワーク中にあるHisの'tug-of-war’による構造安定性変化2014

    • Author(s)
      栃尾直哉,Xu Ning, 玉利佑,津田亮,楯 真一
    • Organizer
      第53回NMR討論会
    • Place of Presentation
      大阪
    • Year and Date
      2014-11-04 – 2014-11-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] プロリン異性化酵素Pin1のドメイン間接触頻度による機能制御2014

    • Author(s)
      Tochio,N., Kawasaki,R., Tamari,Y., Tate,S.
    • Organizer
      日本生物物理学会 年会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Transient folding and functional roles of the intrinsically disordered proteins in gene regulatory proteins2014

    • Author(s)
      Shin-ichi Tate
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      Boston, USA
    • Year and Date
      2014-07-06 – 2014-07-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Functional significance of transient folding in the intrinsically disordered proteins (IDPs)2014

    • Author(s)
      Shin-ichi Tate
    • Organizer
      Euromar2014
    • Place of Presentation
      Zurich, Switzerland
    • Year and Date
      2014-06-29 – 2014-07-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ヒストンシャペロンタンパク質HMGB1の酸化型構造のNMR解析2014

    • Author(s)
      Wang,J., Tochio,N., Takeuchi,A., Uewaki,J., Tate,S.
    • Organizer
      日本生物物理学会 中国四国支部大会
    • Place of Presentation
      鳥取
    • Year and Date
      2014-05-17 – 2014-05-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] プロリン異性化酵素Pin1と二価型リガンドとの結合様式解析2014

    • Author(s)
      栃尾直哉,玉利佑,楯 真一
    • Organizer
      日本生物物理学会 中国四国支部大会
    • Place of Presentation
      鳥取
    • Year and Date
      2014-05-17 – 2014-05-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structural dynamics intrinsically detuning enzyme action revealed by NMR2014

    • Author(s)
      Shin-ichi Tate
    • Organizer
      AnalytiX
    • Place of Presentation
      Dalian, China
    • Year and Date
      2014-04-25 – 2014-04-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://www.mls.sci.hiroshima-u.ac.jp/biophys/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] TateLab Home

    • URL

      http://www.mls.sci.hiroshima-u.ac.jp/biophys/index.html

    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] ナノピペット及びその作成方法2014

    • Inventor(s)
      高見知秀,楯 真一 他
    • Industrial Property Rights Holder
      高見知秀,楯 真一 他
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-220411
    • Filing Date
      2014-10-29
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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