| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
Fertilization triggers cell remodeling from each gamete to a totipotent zygote. In this study, we used the nematode C. elegans to understand the mechanisms underlying the cell remodeling during early development. We isolated several C. elegans mutants, which are defective in the cell remodeling during embryogenesis and found that one of these mutants has a mutation in the farnesyl pyrophosphate synthase gene. We also identified REI-1 as a novel regulator for a small GTPase Rab11 and showed that REI-1 functions as a guanine nucleotide exchange factor to recruit Rab11 to the Golgi apparatus for promoting the cytokinesis of embryos. In addition, we established a live-cell imaging system for mouse embryos and revealed that the cell remodeling such as the selective degradation of maternal membrane proteins also takes place in mammalian embryos.
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