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Molecular mechanism of mitochondrial autophagy

Research Project

Project/Area Number 26291039
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Cell biology
Research InstitutionNiigata University

Principal Investigator

KANKI Tomotake  新潟大学, 医歯学系, 教授 (50398088)

Co-Investigator(Renkei-kenkyūsha) YAMASHITA Shun-ichi  新潟大学, 医歯学総合研究科, 助教 (30529095)
SAIGUSA Tetsu  新潟大学, 医歯学総合研究科, 研究員 (50421954)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsミトコンドリア / オートファジー / 生体分子
Outline of Final Research Achievements

We have studied the molecular mechanism of mitochondria autophagy or mitophagy in yeast and mammalian cells. We found that two MAP kinases, Erk2 and p38, are required for mitophagy in mammalian cells. We also found that the Sin3-Rpd3 complex suppresses the expression of mitophagy essential factor Atg32 under the regulation of Tor in yeast. In addition, we found that when isolation membrane enwraps mitochondria, it contributes to divide mitochondria to the suitable size for enwrapping during mitophagy.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014 Other

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Acknowledgement Compliant: 6 results,  Open Access: 5 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results) Remarks (2 results)

  • [Journal Article] Detection of Hypoxia-Induced and Iron Depletion-Induced Mitophagy in Mammalian Cells.2017

    • Author(s)
      Yamashita SI, Kanki T.
    • Journal Title

      Methods Mol Biol.

      Volume: 印刷中 Pages: 141-149

    • DOI

      10.1007/7651_2017_19

    • ISBN
      9781493977499, 9781493977505
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants.2017

    • Author(s)
      Furukawa K, Kanki T.
    • Journal Title

      Methods Mol Biol.

      Volume: 印刷中 Pages: 95-104

    • DOI

      10.1007/7651_2017_13

    • ISBN
      9781493977499, 9781493977505
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy.2016

    • Author(s)
      Yamashita SI, Jin X, Furukawa K, Hamasaki M, Nezu A, Otera H, Saigusa T, Yoshimori T, Sakai Y, Mihara K, Kanki T.
    • Journal Title

      J Cell Biol.

      Volume: 215 Issue: 5 Pages: 649-665

    • DOI

      10.1083/jcb.201605093

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Constitutive activation of PINK1 leads to proteasome-mediated and non-apoptotic cell death independently of mitochondrial autophagy2016

    • Author(s)
      Akabane, S., Matsuzaki, K., Yamashita, S-I, Arai, K., Okatsu, K., Kanki, T., Matsuda, N., and Oka, T.
    • Journal Title

      J. Biol. Chem.

      Volume: 291 Issue: 31 Pages: 16162-16174

    • DOI

      10.1074/jbc.m116.714923

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)2016

    • Author(s)
      Klionsky DJ., Abdelmohsen K., Abe A., Abedin MJ.,Inomata M., et al.
    • Journal Title

      Autophagy

      Volume: 12(1) Issue: 1 Pages: 1-222

    • DOI

      10.1080/15548627.2015.1100356

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The Molecular Mechanisms and Physiological Role of Mitophagy in Yeast2016

    • Author(s)
      古川健太郎、神吉智丈
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 54 Issue: 4 Pages: 266-272

    • DOI

      10.1271/kagakutoseibutsu.54.266

    • NAID

      130005464585

    • ISSN
      0453-073X, 1883-6852
    • Related Report
      2015 Annual Research Report
  • [Journal Article] Mitophagy is primarily due to alternative autophagy and requires MAPK1 and MAPK14 signaling pathway.2015

    • Author(s)
      Hirota, Y.,Yamashita, S., Kurihara, Y., Jin, X., Aihara, M., Saigusa, T., Kang, D., and Kanki, T.
    • Journal Title

      Autophagy

      Volume: 11 Issue: 2 Pages: 332-43

    • DOI

      10.1080/15548627.2015.1023047

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mitophagy in yeast: Molecular mechanisms and physiological role.2015

    • Author(s)
      Kanki T, Furukawa K, Yamashita SI.
    • Journal Title

      Biochim Biophys Acta.

      Volume: 印刷中 Issue: 10 Pages: 2756-2765

    • DOI

      10.1016/j.bbamcr.2015.01.005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast.2014

    • Author(s)
      Aihara M, Jin X, Kurihara Y, Yoshida Y, Matsushima Y, Oku M, Hirota Y, Saigusa T, Aoki Y, Uchiumi T, Yamamoto T, Sakai Y, Kang D, Kanki T.
    • Journal Title

      Journal of Cell Science

      Volume: 127 Pages: 3184-96

    • DOI

      10.1242/jcs.153254

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Assays for autophagy II: Mitochondrial autophagy.2014

    • Author(s)
      Kanki T, Okamoto K.
    • Journal Title

      Methods Mol Biol.

      Volume: 1163 Pages: 165-173

    • DOI

      10.1007/978-1-4939-0799-1_11

    • ISBN
      9781493907984, 9781493907991
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Drp1-independent mitochondrial division for mitophagy occurs concurrently with autophagosome formation2016

    • Author(s)
      Tomotake Kanki
    • Organizer
      The 4th Sino-Japan Symposium on Autophagy
    • Place of Presentation
      北京市(中国)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mitochondria autophagy in yeast and mammalian cells2015

    • Author(s)
      Tomotake Kanki
    • Organizer
      A3 Autophagy Conference, Korea 2015
    • Place of Presentation
      韓国ソウル
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The signaling pathway regulating mitochondria autophagy2015

    • Author(s)
      Tomotake Kanki
    • Organizer
      2nd International Symposium on Protein Modifications in Pathogenic Dysregulation of Signaling
    • Place of Presentation
      東大医科研
    • Year and Date
      2015-01-23 – 2015-01-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Signaling pathways regulating mitophagy in yeast2014

    • Author(s)
      Tomotake Kanki
    • Organizer
      2014 Northeastern Asian Symposium on Autophagy
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2014-12-18 – 2014-12-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Remarks] 新潟大学大学院医歯学総合研究科  機能制御学分野

    • URL

      http://www.med.niigata-u.ac.jp/mit/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 新潟大学大学院医歯学総合研究科機能制御学分野

    • URL

      http://www.med.niigata-u.ac.jp/mit/

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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