Light-dependent reproductive induction in land plants

• Project/Area Number: 26291059
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: KOHCHI Takayuki 京都大学, 生命科学研究科, 教授 (40202056)
• Co-Investigator(Renkei-kenkyūsha): NISHIHAMA Ryuichi 京都大学, 大学院生命科学研究科, 准教授 (70283455) ; YAMAOKA Shohei 京都大学, 大学院生命科学研究科, 助教 (00378770)
• Research Collaborator: KUBOTA Akane ; INOUE Keisuke
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 植物発生学 / ゼニゴケ / 生殖成長 / フィトクロム / 成長相転換 / 有性生殖 / 植物 / 分子遺伝学 / 環境応答 / 植物分子遺伝学 / 陸上植物進化 / 植物発生 / 苔類ゼニゴケ / 植物環境応答

Outline of Final Research Achievements

In land plants, photoperiod and light quality are major environmental factors to control growth phase transition. However, little is known about how plants evolved regulatory mechanisms in land plant evolution. Comparative genome analysis revealed that acquisition of circadian clock can be traced back to charophytes. Molecular genetic analysis using the model liverwort Marchantia polymorpha showed that the clocks were functional for rhythmic gene expression. The protein complex consisting of GI and FKF functioned downstream of the clocks, and controlled growth phase transition in the gametophyte generation. M. polymorpha has photoreceptor phytochrome and PIF as single copy genes, interacting to transduce red-light signaling. These findings suggest that the basic molecular mechanisms for light-dependent growth phase control were already established at the early stage of plant evolution.

Research Products

• [Int'l Joint Research] メンデル研究所(オーストリア)
• [Int'l Joint Research] ウプサラ大学(スウェーデン)
• [Journal Article] Evolutionary origin of phytochrome responses and signaling in land plants (2017)
  • Author(s): Inoue, K., Nishihama, R., and Kohchi, T.
  • Journal Title: Plant Cell Environ Volume: 印刷中
  • DOI: 10.1111/pce.12908
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Early evolution of the land plant circadian clock (2017)
  • Author(s): 117.Linde, A. M.*, Eklund, D. M.*, Kubota, K.*, Pederson, E. R. A., Holm, K., Gyllenstrand, N., Nishihama, R., Cronberg, N., Muranaka, T., Oyama, T., Kohchi, T., and Lagercrantz, U. * co-first
  • Journal Title: New Phytologist Volume: 印刷中 Pages: 569-590
  • DOI: 10.1111/nph.14487
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phytochrome signaling is mediated by PHYTOCHROME INTERACTING FACTOR in the liverwort Marchantia polymorpha (2016)
  • Author(s): Inoue, K., Nishihama, R., Kataoka, H., Hosaka, M., Manabe, R., Nomoto, M., Tada, Y., Ishizaki, K., and Kohchi, T.
  • Journal Title: Plant Cell Volume: 28 Pages: 1406-1421
  • DOI: 10.1105/tpc.15.01063
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Auxin-mediated transcriptional system with a minimal set of components is critical for morphogenesis through the life cycle in Marchantia polymorpha (2015)
  • Author(s): Kato, H., Ishizaki, K., Kouno, M., Shirakawa, M., Bowman, J.L., Nishihama, R., and Kohchi, T.
  • Journal Title: PLOS Genet Volume: In press
  • DOI: 10.1371/journal.pgen.1005084
  • NAID: 120005672595
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phytochrome-mediated regulation of cell division and growth during regeneration and sporeling development in the liverwort Marchantia polymorpha (2015)
  • Author(s): Nishihama, R., Ishizaki, K., Hosaka, M., Matsuda, Y., Kubota, A., and Kohchi, T.
  • Journal Title: PLOS Genetics Volume: 128 Pages: 407-421
  • DOI: 10.1007/s10265-015-0724-9
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Co-option of a photoperiodic growth-phase transition system during land plant evolution. (2014)
  • Author(s): Akane Kubota, Shogo Kita, Kimitsune Ishizaki, Ryuichi Nishihama, Katsuyuki T. Yamato, Takayuki Kohchi
  • Journal Title: Nature Communication Volume: 5 : 3668 Pages: 3668-3668
  • DOI: 10.1038/ncomms4668
  • NAID: 120005466589
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] VASCULAR PLANT ONE-ZINC FINGER1 and VOZ2 repress the FLOWERING LOCUS C clade members to control flowering time in Arabidopsis (2014)
  • Author(s): Yasui, Y. and Kohchi, T.
  • Journal Title: Biotechnol. Biochem. Volume: 78 Pages: 1850-1855
  • DOI: 10.1080/09168451.2014.932670
  • NAID: 120005676666
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] CYCLING DOF FACTOR ホモロクはゼニゴケの生殖器の発達を抑制する (2017)
  • Author(s): 吉竹良洋，山岡尚平，永山啓太郎，久保田茜，西浜竜一，河内孝之
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] Evolution of phytochrome signaling in land plants (2016)
  • Author(s): Kohchi, T.
  • Organizer: 2016 Annual Meeting and Symposium of Taiwan Society of Plant Biology
  • Place of Presentation: 台湾台中
  • Year and Date: 2016-11-26
  • Int'l Joint Research / Invited
• [Presentation] Evolutionarily conserved light-dependent transcriptional regulatory mechanism in the liverwort Marchantia polymorpha (2016)
  • Author(s): KeiKeisuke Inoue, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi
  • Organizer: EMBO Workshop New model systems for early land plant evolution
  • Place of Presentation: オーストリア メンデル研究所
  • Year and Date: 2016-06-22
  • Int'l Joint Research
• [Presentation] The transcription factor BONOBO plays a central role in transition from vegetative to reproductive growth in the liverwort Marchantia polymorpha (2016)
  • Author(s): Shohei Yamaoka, Keisuke Inoue, Ryuichi Nishihama, Katsushi Yamaguchi, Shuji Shigenobu, Kimitsune Ishizaki, Katsuyuki T. Yamato, Takayuki Kohchi
  • Organizer: EMBO Workshop New model systems for early land plant evolution
  • Place of Presentation: オーストリア メンデル研究所
  • Year and Date: 2016-06-22
  • Int'l Joint Research
• [Presentation] Genome and Genomics in Marchantia polymorpha (2016)
  • Author(s): Takayuki Kohchi, Katsuyuki Yamato, Kimitsune Ishizaki, Shohei Yamaoka, Ryuichi Nishihama, John Bowman
  • Organizer: EMBO Workshop New model systems for early land plant evolution
  • Place of Presentation: オーストリア メンデル研究所
  • Year and Date: 2016-06-22
  • Int'l Joint Research / Invited
• [Presentation] The liverwort, Marchantia polymorpha; A powerful model of basal land plants for plant science and biotechnology (2016)
  • Author(s): Kohchi, T.
  • Organizer: 2016 Annual meeting of the Korean Society for Plant Biotechnology
  • Place of Presentation: 韓国プサン
  • Year and Date: 2016-06-09
  • Int'l Joint Research / Invited
• [Presentation] 苔類ゼニゴケの成長相制御因子BONOBOの日長・光質による発現制御 (2016)
  • Author(s): 山岡尚平、井上佳祐、友金寛和、西浜竜一、山口勝司、重信秀治、石崎公庸、大和勝幸、河内孝之
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] 苔類ゼニゴケにおいて遺伝的に保存されたGI-FKF-CDF経路は光周期性成長相転換を決定する (2016)
  • Author(s): 永山啓太郎、吉竹良洋、久保田茜、西浜竜一、山岡尚平、河内孝之
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] 苔類ゼニゴケにおける転写因子PIFを介した赤色光シグナル伝達の分子機構 (2016)
  • Author(s): 井上佳祐、西浜竜一、石崎公庸、河内孝之
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] 苔類ゼニゴケを用いた転写因子PIFによる赤色光シグナル伝達機構の解析 (2015)
  • Author(s): 井上佳祐、西浜竜一、石崎公庸、河内孝之
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 転写因子BONOBOは苔類ゼニゴケの有性生殖器官形成を制御する (2015)
  • Author(s): 山岡尚平、友金寛和、西浜竜一、山口勝司、重信秀治、石崎公庸、大和勝幸、河内孝之
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 苔類ゼニゴケの光質および日長による成長相転換機構 (2014)
  • Author(s): 河内孝之
  • Organizer: 日本植物学会大会
  • Place of Presentation: 明治大学（東京都）
  • Year and Date: 2014-09-12 – 2014-09-14
  • Invited
• [Presentation] 基部陸上植物ゼニゴケにおけるGI-FKF複合体を介した光周性生長相制御機構 (2014)
  • Author(s): 久保田茜、喜多祥吾、永山啓太郎、山岡尚平、西浜竜一、石崎公庸、河内孝之
  • Organizer: 日本植物学会大会
  • Place of Presentation: 明治大学（東京都）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 苔類ゼニゴケにおいてCYCLING DOF FACTORは光周性成長相転換を抑制する (2014)
  • Author(s): 永山啓太郎、久保田茜、喜多祥吾、山岡尚平、西浜竜一、石崎公庸、河内孝之
  • Organizer: 日本植物学会大会
  • Place of Presentation: 明治大学（東京都）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Remarks] 京都大学生命科学研究科 遺伝子特性学分野
  • URL: http://www.plantmb.lif.kyoto-u.ac.jp/
• [Remarks] 京都大学生命科学研究科遺伝子特性学分野ウェブページ
  • URL: http://www.plantmb.lif.kyoto-u.ac.jp/