Gene regulatory network underlying plant organ growth

• Project/Area Number: 26291064
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Sugimoto Keiko 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (30455349)
• Co-Investigator(Renkei-kenkyūsha): Breuer Christian 国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (90525859) ; HARASHIMA Hirofumi 国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (00700113)
• Research Collaborator: Rymen Bart 国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (80737255)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 植物ホルモン・成長生理・全能性 / 細胞分化 / 細胞周期 / 遺伝子発現調節 / 発生遺伝

Outline of Final Research Achievements

Plant organ growth is mediated by a balanced progression of cell division and differentiation. Plant cells udergo massive transcriptional changes when they transit from proliferateive to differentiation phases but how these modifications in gene expression are regulated is not well understood. From a new genetic screen we have recently identified several putative transcriptional regulators that might mediate these transcriptional responses. In this study we investigated functional roles of these new regulators and we showed that a DOF transcriptional factor OBP4 negatively regulates root hair growth.

Research Products

• [Journal Article] ABA Suppresses Root Hair Growth via the OBP4 Transcriptional Regulator. (2017)
  • Author(s): Rymen, B., Kawamura, A., Schafer, S., Breuer, C., Iwase, A., Shibata, M., Ikeda, M., Mitsuda, N., Koncz, C., Ohme-Takagi, M., Matsui, M., and Sugimoto, K.
  • Journal Title: Plant physiology Volume: 173 Issue: 3 Pages: 1750-1762
  • DOI: 10.1104/pp.16.01945
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Shoot removal induces chloroplast development in roots via cytokinin signaling (2017)
  • Author(s): Koichi Kobayashi, Ai Ohnishi, Daichi Sasaki, Sho Fujii, Akira Iwase, Keiko Sugimoto, Tatsuru Masuda and Hajime Wada
  • Journal Title: Plant Physiology Volume: 173 Issue: 4 Pages: 2340-2355
  • DOI: 10.1104/pp.16.01368
  • Peer Reviewed / Open Access
• [Journal Article] Molecular networks orchestrating plant cell growth. (2017)
  • Author(s): Franciosini, A., Rymen, B., Shibata, M., Favero, D.S., and Sugimoto, K.
  • Journal Title: Current opinion in plant biology Volume: 35 Pages: 98-104
  • DOI: 10.1016/j.pbi.2016.11.010
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis. (2016)
  • Author(s): Weimer, A.K., Biedermann, S., Harashima, H., Roodbarkelari, F., Takahashi, N., Foreman, J., Guan, Y., Pochon, G., Heese, M., Van Damme, D., Sugimoto, K., Koncz, C., Doerner, P., Umeda, M., Schnittger, A.
  • Journal Title: EMBO J Volume: 35 Issue: 19 Pages: 2068-2086
  • DOI: 10.15252/embj.201593083
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] SUPPRESSOR OF PHYTOCHROME B4-#3 represses genes associated with auxin signaling to modulate hypocotyl growth. (2016)
  • Author(s): Favero DS, Jacques CN, Iwase A, Le KN, Zhao J, Sugimoto K, Neff MM.
  • Journal Title: Plant Physiol Volume: 171(4) Issue: 4 Pages: 2701-2716
  • DOI: 10.1104/pp.16.00405
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Integration of developmental and environmental signals into cell proliferation and differentiation through RETINOBLASTOMA-RELATED 1 (2016)
  • Author(s): Harashima H and Sugimoto K.
  • Journal Title: Curr Opin in Plant Biol Volume: 15 Pages: 683-690
  • DOI: 10.1016/j.pbi.2015.12.003
  • Peer Reviewed
• [Journal Article] Functional conservation in the SIAMESE-RELATED family of Cyclin-dependent kinase inhibitors in land plants (2015)
  • Author(s): Kumar N, Harashima H, Kalve S, Bramsiepe J, Wang K, Sizani BL, Bertrand LL, Johnson MC, Faulk C, Dale R, Simmons LA, Churchman ML, Sugimoto K, Kato N, Dasanayake M, Beemster G, Schnittger A, Larkin JC
  • Journal Title: Plant Cell Volume: 27 Issue: 11 Pages: 3065-3080
  • DOI: 10.1105/tpc.15.00489
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcriptional repression by MYB3R proteins regulates plant organ growth. (2015)
  • Author(s): Kobayashi K, Suzuki T, Iwata E, Nakamichi N, Suzuki T, Chen P, Ohtani M, Ishida T, Hosoya H, Müller S, Leviczky T, Pettkó-Szandtner A, Darula Z, Iwamoto A, Nomoto M, Tada Y, Higashiyama T, Demura T, Doonan JH, Hauser MT, Sugimoto K, Umeda M, Magyar Z, Bögre L, Ito M
  • Journal Title: EMBO Journal Volume: 34 Issue: 15 Pages: 1992-2007
  • DOI: 10.15252/embj.201490899
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Stimulation of cell elongation by tetraploidy in hypocotyls of dark-grown Arabidopsis seedlings. (2015)
  • Author(s): Narukawa, H., Yokoyama, R., Komaki, S., Sugimoto, K., Nishitani, K.
  • Journal Title: PLoS One Volume: 10 Issue: 8 Pages: e0134547-e0134547
  • DOI: 10.1371/journal.pone.0134547
  • Peer Reviewed
• [Journal Article] Differential regulation of B2-type CDK accumulation in Arabidopsis roots (2014)
  • Author(s): Okushima, Y., Shimizu, K., Ishida, T., Sugimoto, K. and Umeda, M.
  • Journal Title: Plant Cell Rep. Volume: 33 Issue: 7 Pages: 1033-1040
  • DOI: 10.1007/s00299-014-1581-z
  • NAID: 120006537389
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Endocycling in the path of plant development. (2014)
  • Author(s): Breuer C, Braidwood L, Sugimoto K
  • Journal Title: Current Opinion in Plant Biology Volume: 17 Pages: 78-85
  • DOI: 10.1016/j.pbi.2013.11.007
  • Peer Reviewed
• [Journal Article] My body is a cage: Mechanisms and modulation of plant cell growth (2014)
  • Author(s): Braidwood L, Breuer C, Sugimoto K.
  • Journal Title: New Phytologist, Volume: 201(2) Issue: 2 Pages: 388-402
  • DOI: 10.1111/nph.12473
  • Peer Reviewed
• [Presentation] Two transcription factors govern root hair growth in Arabidopsis (2017)
  • Author(s): Shibata M, Breuer C,Kawamura A, Rymen B, Watt L, Clark N, Braidwood L, Sozzani R, Benfey P, Sugimoto K
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学、鹿児島県
  • Year and Date: 2017-03-16
• [Presentation] A trihelix transcription factor GTL1 regulates root hair development in Arabidopsis (2016)
  • Author(s): Shibata M, Breuer C,Kawamura A, Rymen B, Watt L, Clark N, Braidwood L, Sozzani R, Benfey P, Sugimoto K
  • Organizer: Latest Advances in Plant Development & Environmental Response, CSH Conferences Asia
  • Place of Presentation: 淡路夢舞台、兵庫県淡路市
  • Year and Date: 2016-11-29
  • Int'l Joint Research
• [Presentation] シロイヌナズナRETINOBLASTOMA-RELATED PROTEIN 1に着目したG1-S期制御機構の解析 (2016)
  • Author(s): 原島 洋文
  • Organizer: 第 57 回日本植物生理学会年会
  • Place of Presentation: 岩手大学、岩手
  • Year and Date: 2016-03-18
• [Presentation] Transcriptional control of root hair growth under adverse conditions (2015)
  • Author(s): Bart Rymen
  • Organizer: The 1st meeting of Young Scientists for Integrative system of autonomous environmental signal recognition and memorization for plant plasticity
  • Place of Presentation: ホテルウェルシーズン浜名湖、静岡
  • Year and Date: 2015-11-18
• [Presentation] Retinoblastoma resets the cell cycle after S phase through a system of interconnected feedback loops (2015)
  • Author(s): 原島 洋文
  • Organizer: The 1st meeting of Young Scientists for Integrative system of autonomous environmental signal recognition and memorization for plant plasticity
  • Place of Presentation: ホテルウェルシーズン浜名湖、静岡
  • Year and Date: 2015-11-18
• [Presentation] Cessation of root-hair elongation by phosphate signaling (2015)
  • Author(s): 柴田 美智太郎
  • Organizer: 第1回 環境記憶若手の会
  • Place of Presentation: ホテルウェルシーズン浜名湖、静岡
  • Year and Date: 2015-11-18
• [Presentation] Transcriptional control of root hair growth under adverse conditions (2015)
  • Author(s): Bart Rymen
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium
  • Place of Presentation: 名古屋大学、愛知
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] Transcriptional control of root hair length in Arabidopsis (2015)
  • Author(s): 柴田 美智太郎
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium 2015
  • Place of Presentation: 名古屋大学、愛知
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] Transcriptional control of root hair growth under adverse conditions (2015)
  • Author(s): Bart Rymen
  • Organizer: International Conference of Arabidopsis Research 2015
  • Place of Presentation: Palais des congres, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] Retinoblastoma resets the cell cycle after S phase through a system of interconnected feedback loops (2015)
  • Author(s): 原島 洋文
  • Organizer: International Conference of Arabidopsis Research 2015
  • Place of Presentation: Palais des congres, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] RBR1に着目したG1-S期制御機構の解明 (2015)
  • Author(s): 原島 洋文
  • Organizer: 2015年度細胞周期合同セミナー
  • Place of Presentation: サンヒルズ三河湾
  • Year and Date: 2015-06-12
• [Presentation] 根毛の伸長成長を抑制する転写因子GT-2ファミリーの機能 (2015)
  • Author(s): 柴田 美智太郎
  • Organizer: 2015年度細胞周期合同セミナー
  • Place of Presentation: サンヒルズ三河湾、愛知
  • Year and Date: 2015-06-12
• [Presentation] Chemical genetic approach to identify the substrates of plant cyclin-dependent kinases (2014)
  • Author(s): 原島洋文、Philippe Hammann, 杉本慶子、Arp Schnittger
  • Organizer: The 38th Naito Conference on Molecule-based biological systems
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ（札幌市）
  • Year and Date: 2014-10-07 – 2014-10-10
• [Presentation] To grow or not to grow: plant growth signaling under environmental limiting conditions (2014)
  • Author(s): Bart Rymen, 岩瀬哲、杉本慶子
  • Organizer: The 38th Naito Conference on Molecule-based biological systems
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ（札幌市）
  • Year and Date: 2014-10-07 – 2014-10-10
• [Presentation] Transcriptional regulation of organ growth under limiting conditions (2014)
  • Author(s): Bart Rymen, 池田美穂、光田展隆、高木優、松井南、杉本慶子
  • Organizer: The 25th International Conference on Arabidopsis Research
  • Place of Presentation: University of British Columbia, Canada
  • Year and Date: 2014-07-28 – 2014-08-01
  • Invited
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• [Remarks] Cell Function Research Team
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