Molecular mechanisms underlying heterochromatin assembly
| Project/Area Number |
26291072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | National Institute for Basic Biology (2016-2017)
Nagoya City University (2014-2015) |
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Principal Investigator |
Nakayama Jun-ichi 基礎生物学研究所, クロマチン制御研究部門, 教授 (60373338)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Keywords | 遺伝子 / 発現制御 / ヘテロクロマチン / 分裂酵母 / ユビキチン化 / ユビキチン |
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| Outline of Final Research Achievements |
In this study, we focused on Clr4 methyltransferase complex (CLRC), and investigated a functional link between ubiquitin modifications and heterochromatin assembly. Affinity purified CLRC was shown to ubiquitylate histone H3. We further demonstrated that H3 ubiquitylation was tightly associated with H3K9me-enriched chromatin and that H3 ubiquitylation promotes H3K9 methylation by Clr4.
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Report
(5 results)
Research Products
(14 results)
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[Journal Article] The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin.2017
Author(s)
Mutazono, M., Morita, M., Tsukahara, C., Chinen, M., Nishioka, S., Yumikake, T., Dohke, K., Sakamoto, M., Ideue, T., Wakayama, J.I., Ishii, K., Tani, T.
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Journal Title
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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