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Molecular mechanisms underlying heterochromatin assembly

Research Project

Project/Area Number 26291072
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Genetics/Chromosome dynamics
Research InstitutionNational Institute for Basic Biology (2016-2017)
Nagoya City University (2014-2015)

Principal Investigator

Nakayama Jun-ichi  基礎生物学研究所, クロマチン制御研究部門, 教授 (60373338)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Keywords遺伝子 / 発現制御 / ヘテロクロマチン / 分裂酵母 / ユビキチン化 / ユビキチン
Outline of Final Research Achievements

In this study, we focused on Clr4 methyltransferase complex (CLRC), and investigated a functional link between ubiquitin modifications and heterochromatin assembly. Affinity purified CLRC was shown to ubiquitylate histone H3. We further demonstrated that H3 ubiquitylation was tightly associated with H3K9me-enriched chromatin and that H3 ubiquitylation promotes H3K9 methylation by Clr4.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (14 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 10 results)

  • [Journal Article] Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly2017

    • Author(s)
      Atsuko Shirai, Takayuki Kawaguchi, Hideaki Shimojo, Daisuke Muramatsu, Mayumi Ishida-Yonetani, Yoshifumi Nishimura, Hiroshi Kimura, Jun-ichi Nakayama, Yoichi Shinkai
    • Journal Title

      eLIFE

      Volume: 6

    • DOI

      10.7554/elife.25317

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin.2017

    • Author(s)
      Mutazono, M., Morita, M., Tsukahara, C., Chinen, M., Nishioka, S., Yumikake, T., Dohke, K., Sakamoto, M., Ideue, T., Wakayama, J.I., Ishii, K., Tani, T.
    • Journal Title

      PLoS Genetics

      Volume: 13 Issue: 2 Pages: e1006606-e1006606

    • DOI

      10.1371/journal.pgen.1006606

    • NAID

      120006697213

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly2017

    • Author(s)
      Jun-ichi Nakayama
    • Organizer
      12th Asian Epigenomics Meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高次クロマチン構造形成におけるヒストン修飾のクロストーク2016

    • Author(s)
      大屋恵梨子,田中万葉,西淵剛平,中川れい子,町田晋一,胡桃坂仁志,田上英明,中山 潤一
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 分裂酵母におけるヘテロクロマチン構造形成の分子機構2016

    • Author(s)
      中山潤一
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2016-09-27
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 分裂酵母のヘテロクロマチン形成におけるCLRC複合体の役割2016

    • Author(s)
      大屋恵梨子,田中万葉,西淵剛平,中川れい子,町田晋一,胡桃坂仁志,田上英明,中山 潤一
    • Organizer
      酵母遺伝学フォーラム第49回研究報告会
    • Place of Presentation
      舞子ビラ(兵庫県神戸市垂水区)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] Crosstalk between histone modifications during heterochromatin assembly2016

    • Author(s)
      Jun-ichi Nakayama
    • Organizer
      Kick-Off Symposium in Nagoya City University 2016 "Regulatory Mechanisms of Epigenetic Information and Their Clinical Applications"
    • Place of Presentation
      名古屋市立大学(名古屋市)
    • Year and Date
      2016-02-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒストンのメチル化修飾を維持する分子機構2016

    • Author(s)
      中山潤一
    • Organizer
      第3回ヒストンバリアント研究会
    • Place of Presentation
      早稲田大学(新宿区)
    • Year and Date
      2016-02-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ヘテロクロマチン構造形成におけるCLRC複合体の役割2016

    • Author(s)
      大屋恵梨子、西淵剛平、中川れい子、田中万葉、町田晋一、胡桃坂仁志、田上英明、中山潤一
    • Organizer
      第33回染色体ワークショップ、第14回核ダイナミクス研究会
    • Place of Presentation
      松島一の坊(宮城郡松島町)
    • Year and Date
      2016-01-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Crosstalk between histone modifications during heterochromatin assembly2015

    • Author(s)
      Jun-ichi Nakayama
    • Organizer
      第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Crosstalk between histone modifications during heterochromatin assembly2015

    • Author(s)
      Jun-ichi Nakayama
    • Organizer
      Forefront of Chromosome Biology
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2015-08-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Crosstalk between histone modifications during heterochromatin assembly2015

    • Author(s)
      Eriki Oya, Mayo Tanaka, Gohei Nishibuchi, Reiko Nakagawa, Shinichi Machida, Hitoshi Kurumizaka, Hedeaki Tagami, Jun-ichi Nakayama
    • Organizer
      The 8th International Fission Yeast Meeting
    • Place of Presentation
      生田神社(神戸市)
    • Year and Date
      2015-06-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高次クロマチン構造の形成と維持の分子機構2015

    • Author(s)
      中山潤一
    • Organizer
      2014年度 遺伝研究会「単細胞の増殖メカニズムの先端的研究」
    • Place of Presentation
      国立遺伝学研究所(三島市)
    • Year and Date
      2015-03-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 分裂酵母における高次クロマチン構造の形成と維持の分子機構2014

    • Author(s)
      中山潤一
    • Organizer
      2014年度 遺伝研研究会「クロマチンによるゲノムDNAの機能発現メカニズム」
    • Place of Presentation
      国立遺伝学研究所(三島市)
    • Year and Date
      2014-10-30
    • Related Report
      2014 Annual Research Report
    • Invited

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Published: 2014-04-04   Modified: 2019-03-29  

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