|Budget Amount *help
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
|Outline of Final Research Achievements
Paramecium bursaria is the only experimental system capable of inducing endosymbiosis between eukaryotic cells in synchronization with a large amount of cells. The genes of P. bursaria with markedly altered expression level in the presence or absence of symbiotic Chlorella cells were detected by RNAseq, and the antibodies raised for synthesized petides of the gene products were developed and used to know intracellular localization of the antigens. In addition, an antibody against the perialgal vacuole membrane, which plays an essential role to protect algae from lysosomal fusion in secondary symbiosis, and clarified timing of appearance of the antigen during infection process.