| Project/Area Number |
26291080
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大島 一彦 長浜バイオ大学, バイオサイエンス学部, 准教授 (60282852)
松田 洋一 名古屋大学, 生命農学研究科, 教授 (70165835)
森 哲 京都大学, 理学研究科, 准教授 (80271005)
細 将貴 京都大学, 白眉センター, 特定助教 (80557695)
佐藤 宏 山口大学, 共同獣医学部, 教授 (90211945)
長谷川 英男 大分大学, 医学部, 教授 (00126442)
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| Co-Investigator(Renkei-kenkyūsha) |
OTA Hidetoshi 兵庫県立大学, 自然・環境科学研究所, 教授 (70165835)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Keywords | 遺伝子水平伝播 / 生物系統地理 / 爬虫両棲類 / 寄生虫 / ベクター生物 / マダガスカル / 次世代シークエンス / 分子系統解析 / 転移因子 / 両生類 / 爬虫類 / 魚類 / 分子系統 / 次世代シークエンサー / 系統地理 / ゲノム生物学 / 系統分類学 / 遺伝子の水平伝播 / 国際研究者交流_アフリカ_ドイツ / 国際情報交換_アフリカ_アメリカなど / ヘビ類 / 生物地理学 |
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| Outline of Final Research Achievements |
We had discovered a unique LINE retrotransposon (hereinafter TE-X) that horizontally transferred from snake to frog. In this study, we aimed to elucidate the geographic areas and phylogenetic origins of the horizontal transfer (HT) events and identify the vector organisms intermediate the HT. We collected 194, 139, and 166 specimens from 29 anuran families, 17 snake families, and parasite and blood-sacking invertebrate taxa of five animal phyla, respectively. We PCR amplified the TE-X from these specimens and analyzed them using PCR and next generation sequencer. Finally we performed molecular phylogenetic analyses of the resultant TE-X sequences. As the results, we revealed the following matters. The TE-X HTs have occurred many times in various parts of the world and the transfer would be mediated by various parasites and blood sacking animals. Remarkably, our data seem to show that the TE-X HT is continuing among many vertebrate taxa in Madagascar.
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