Comprehensive research on Takakia and Haplomitrium based on genomic information

• Project/Area Number: 26291081
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Hiroshima University
• Principal Investigator: Shimamura Masaki 広島大学, 理学研究科, 准教授 (00432708)
• Co-Investigator(Kenkyū-buntansha): 西山 智明 金沢大学, 学際科学実験センター, 助教 (50390688) ; 榊原 恵子 立教大学, 理学部, 准教授 (90590000)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 進化 / コケ植物 / 比較ゲノム / 発生進化 / 植物 / ナンジャモンジャゴケ / コマチゴケ

Outline of Final Research Achievements

Comprehensive studies based on genome information of Takakia and Haplomitrium were conducted. Complete sequence of chloroplast genome of T. lepidozioides was annotated and submitted to the database with accession number NC_028738.1. DNA barcoding and phylogenetic analysis of isolated fungi hyphae in mucilage hairs of Takakia lepidozioide indicated that the fungi forming the labyrinthine structure are closely related to Helotiales species isolated from the roots of the Ericaceae species. Although Haplomitrium mnioides has a relatively large genome size (ca. 4.4Gb), it shows relative simplicity of genetic networks, like a model liverwort Marchantia polymoprpha.

Research Products

• [Journal Article] Spores of Relictual Bryophytes: Diverse Adaptations to Life on Land (2015)
  • Author(s): Brown, R. C., Lemmon, B. E., Shimamura, M., Villarreal, J. C., & Renzaglia, K. S.
  • Journal Title: Rev. Palaeobot. Palynol. Volume: 216 Pages: 1-17
  • DOI: 10.1016/j.revpalbo.2015.01.004
  • Peer Reviewed
• [Presentation] Morphological Study on the vegetative propagation by deciduous leaves in Takakia lepidozioides (2017)
  • Author(s): Tianxiong Zheng , Maki Murakami , Yukari Kuga , Masaki Shimamura
  • Organizer: XIX International Botanical Congress, Shenzen, China.
  • Int'l Joint Research
• [Presentation] The role of infection of symbiotic fungi through the asexual reproduction by deciduous leaves of Takakia lepidozioides (2017)
  • Author(s): Zheng Tianxiong, Maki Murakami, Yukari Kuga, Masaki Shimamura
  • Organizer: 日本蘚苔類学会 第46回群馬大会
• [Presentation] コケ植物の精子形成過程と細胞骨格 (2017)
  • Author(s): 嶋村正樹
  • Organizer: 日本植物学会
• [Presentation] ナンジャモンジャゴケにおける真菌との菌根様構造の形成 (2016)
  • Author(s): 村上真祈・久我ゆかり・嶋村正樹
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄県
  • Year and Date: 2016-09-16
• [Presentation] Takakia and Haplomitrium, as the model for studying the earliest evolution of land plants. (2016)
  • Author(s): Masaki Shimamura, Tomoaki Nishiyama, Keiko Sakakibara
  • Organizer: EMBO Workshop: New model systems for early land plant evolution
  • Place of Presentation: ウィーン，オーストリア
  • Year and Date: 2016-07-22
  • Int'l Joint Research
• [Presentation] セン類ナンジャモンジャゴケと共生菌の形態学的研究 (2015)
  • Author(s): 村上真祈・久我ゆかり・嶋村正樹
  • Organizer: 日本蘚苔類学会第44回大会
  • Place of Presentation: 長野県
  • Year and Date: 2015-08-04
• [Presentation] ナンジャモンジャゴケと共生菌の形態学的研究. (2015)
  • Author(s): 村上真祈・久我ゆかり・山口富美夫・嶋村正樹
  • Organizer: 中国四国地区生物系三学会合同大会
  • Place of Presentation: 愛媛県
  • Year and Date: 2015-05-16
• [Presentation] セン類ナンジャモンジャゴケの粘液毛からの共生菌の侵入 (2014)
  • Author(s): 村上真祈・久我ゆかり・嶋村正樹
  • Organizer: 日本植物形態学会
  • Place of Presentation: 神奈川県
  • Year and Date: 2014-09-11
• [Book] 植物の世代交代制御因子の発見 (2016)
  • Author(s): 榊原恵子
  • Total Pages: 190
  • Publisher: 慶應義塾大学出版会