| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
The grasses (Poaceae) possess a unique self-incompatibility system controlled by two unlinked loci S and Z. This study aimed to isolate and identify an unknown pollen (male) S gene in a wild species of barley (Hordeum bulbosum). Informatics analysis using orthologous sequences of the already-identified pistil (female) S gene successfully delimited the genomic region homologous to the S locus on the barley and wheat chromosomes. Among the H. bulbosum orthologs residing within the delimited region, a promising pollen S gene candidate was finally identified based on physical linkage to the female S gene, sequence polymorphisms and preferential expression in the pollen.
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