The study of the interaction between Magnaportha effector AVR-Pik and rice HMA domain protein
Project/Area Number |
26292027
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Plant protection science
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Research Institution | Iwate Biotechnology Research Center |
Principal Investigator |
Kanzaki Hiroyuki 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究専門員 (10390882)
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Co-Investigator(Renkei-kenkyūsha) |
TERAUCHI Ryohei 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究部長 (50236981)
SAITOH Hiromasa 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 主任研究員 (20414336)
FUJISAKI Koki 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (30626510)
TAKEDA Takumi 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 主任研究員 (80423036)
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Research Collaborator |
ITO Kazue 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究助手
KANZAKI Eiko 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 研究技術員
Kamoun Sophien The Sainsbury Laboratory, Professor
Banfield Mark John Innes Centre, Department of Biological Chemistry, Professor
Kroj Thomas INRA, UMR, BGPI, Professor
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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Keywords | HMAドメイン / エフェクター遺伝子 / いもち病抵抗性遺伝子 / 活性酸素種 / 罹病性 / 酵母2ハイブリッド法 / イネ / いもち病菌 / ROS / いもち病菌エフェクター / 罹病性遺伝子 / いもち病菌エフェクターAVR-Pik / sHMA / エフェクター / AVR-Pik / タンパク質相互作用 |
Outline of Final Research Achievements |
The rice R-gene protein Pik-1 and RGA5 have an integrated Heavy Metal-Associated (HMA) domain that directly binds secreted effector proteins from the rice blast fungus Magnaporthe oryzae. The HMA domain is also found indefines a family of small proteins of rice designated as small HMA-containing proteins (sHMAs). We discovered that the M. oryzae cytoplasmic effector AVR-Pik-D binds six members of the sHMA family, and stabilizes sHMA1 in planta. Knockdown of sHMA genes in rice enhanced infection by a compatible isolate of M. oryzae, indicating that sHMAs are required for rice susceptibility to blast infection, and can therefore be considered as susceptibility (S-) genes. sHMA proteins negatively regulate the accumulation of reactive oxygen species (ROS), potentially by interacting with a protein that scavenges ROS. Our findings led us to propose an evolutionary model in which M. oryzae evolved the AVR-Pik effector to bind and stabilize sHMA proteins and increase host susceptibility.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] A chloroplast-localized protein LESION AND LAMINA BENDING affects defense and growth responses in rice.2016
Author(s)
Tamiru, M., Takagi, H., Abe, A., Yokota, T., Kanzaki, H., Okamoto, H., Saitoh, H., Takahashi, H., Fujisaki, K., Oikawa, K., Uemura, A., Natsume, S., Jikumaru, Y., Matsuura, H., Umemura, K., Terry, M. J., Terauchi, R.
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Journal Title
New Phytologist
Volume: 210
Issue: 4
Pages: 1282-1297
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Whole genome sequencing approaches to understand Magnaporthe-rice interactions.2016
Author(s)
Terauchi, R., Kanzaki, H., Fuijsaki, K., Takagi, H., Abe, A., Yoshida, K., Okuyama, Y., Tamiru, M., Saitoh, H.
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Journal Title
Physiological and Molecular Plant Pathology
Volume: 94
Pages: 1-5
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Rice Exo70 interacts with a fungal effector, AVR-Pii and is required for AVR-Pii-triggered immunity2015
Author(s)
Fujisaki, K., Abe, Y., Ito, A., Saitoh, H., Yoshida, K., Kanzaki H., Kanzaki, E., Utsushi, H., Yamashita, T., Kamoun, S. and Terauchi, R.
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Journal Title
The Plant Journal
Volume: 83
Issue: 5
Pages: 875-887
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor.2015
Author(s)
Maqbool, A., Saitoh, H., Franceschetti, M., Stevenson, C.E.M., Uemura, A., Kanzakii, H., Kamoun, S., Terauchi, R., Banfield, M. J.
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Journal Title
eLife
Volume: 4
Pages: 1-24
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] MutMap accelerates breeding of a salt-tolerant rice cultivar2015
Author(s)
Takagi, H., Tamiru, M., Abe, A., Yoshida, K., Uemura, A., Yaegashi, H., Obara, T., Oikawa, K., Utsushi, H., Kanzaki, E., Mitsuoka, C., Natsume, S., Kosugi, S., Kanzaki, H., Matsumura, H., Urasaki, N., Kamoun, S., Terauchi, R.
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Journal Title
Nature Biotechnology
Volume: 33
Issue: 5
Pages: 445-449
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The rice (Oryza saliva L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response2014
Author(s)
Fekih, R., Tamiru, M., Kanzaki, H., Abe, A., Yoshida, K., Kanzaki, E., Saitoh, H., Takagi, H., Natsume, S., Undan, J. R., Undan, J, Terauchi, R.
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Journal Title
Molecular Genetics and Genomics
Volume: 290
Issue: 2
Pages: 611-622
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance2014
Author(s)
Cesari, S., Kanzaki, H., Fujiwara, T., Bernoux, M., Chalvon, V., Kawano, Y., Shimamoto, K., Dodds, P., Terauchi, R., Kroj, T.
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Journal Title
EMBO Journal
Volume: 33
Issue: 17
Pages: 1941-1959
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Book] Chapter 3 (Whole Genome Sequencing to Identify Genes and QTL in Rice) in Advances in the Understanding of Biological Sciences Using Next Generation Sequencing (NGS) Approaches2015
Author(s)
Terauchi R., Abe A., Takagi H., Tamiru M., Fekih R., Natsume S., Yaegashi H., Kosugi S., Kanzaki H., Matsumura H., Saitoh H., Yoshida K., Cano L., Kamoun S.
Publisher
Springer
Related Report
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[Book] Methods in Molecular Biology, Vol. 1127, Chapter 202014
Author(s)
Kanzaki, H., Yoshida, K., Saitoh, H., Tamiru, M. and Terauchi, R.
Publisher
Springer
Related Report