| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Outline of Final Research Achievements |
Based on the correlation between the temporal pattern of NADPH oxidase/Rboh-mediated production of reactive oxygen species (ROS) and downstream defense responses including hypersensitive cell death, we established a high-throughput screening to identify novel compounds that modulate plant defense responses. From a total of 11,000 compounds screened, we identified compounds that activate ROS production and hypersensitive cell death in BY-2 cells. We report the effects of these chemicals on the MAMP-induced various responses in Arabidopsis including expression of various defense-related genes in planta. We also conducted comparative transcriptomic analysis using RNA-seq to reveal the effects of the chemicals on MAMP-induced gene expression. Results suggest that some chemicals activate different defense signaling pathways distinct from the known plant defense activators that activate the salicylic acid pathway.
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