| Project/Area Number |
26292047
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
OHKUMA Moriya 国立研究開発法人理化学研究所, バイオリソースセンター, 室長 (10270597)
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| Co-Investigator(Kenkyū-buntansha) |
野田 悟子 山梨大学, 総合研究部, 准教授 (80342830)
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| Co-Investigator(Renkei-kenkyūsha) |
HONGOH Yuichi 東京工業大学, 生命理工学研究科, 教授 (90392117)
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| Research Collaborator |
YUKI Masahiro
INOUE Jun-ichi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 微生物機能 / 窒素固定 / 水素代謝 / 共生 / シングルセルゲノム / 還元的酢酸生成 / Bacteroidales目細菌 / スピロヘータ / ゲノム / 生態 / 進化 / 微生物 / 生態学 / 分類学 / 微生物ゲノム / 微生物進化 / 生物多様性 / 難培養微生物 |
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| Outline of Final Research Achievements |
We found bacterial species in the order Bacteroidales that harbor genes for nitrogen fixation or genes homologous to archaea for hydrogen utilization in the genomes, and we detected the respective activities in their representative cultured strains. We identified an endosymbiotic spirochete species of a termite-gut cellulolytic protist species that contributes to the activities for both nitrogen fixation and hydrogen-utilizing reductive acetogenesis in the gut. Through single-cell genome sequencing, we investigated functions and symbiotic mechanisms of this endosymbiont and two ectosymbiotic Bacteroidales species attached onto cell surface of termite-gut protists.
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