Molecular mechanism and application of structural elements that govern carbohydrase-catalyzed transfer reaction
Project/Area Number |
26292049
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Hokkaido University |
Principal Investigator |
KIMURA, Atsuo 北海道大学, (連合)農学研究科(研究院), 教授 (90186312)
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Co-Investigator(Kenkyū-buntansha) |
奥山 正幸 北海道大学, (連合)農学研究科(研究院), 講師 (00344490)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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Keywords | 糖質酵素 / 糖転移反応 / 構造因子 |
Outline of Final Research Achievements |
This project aims at identification and application of structural elements that regulate and improve the carbohydrase-catalyzed transfer activities by elucidating four promising phenomena, which we newly found. Results obtained are as follows: 1) we identify the structural elements to control the α-1,3-transfer reaction displayed by α-1,3-glucoside-transfer enzyme; 2) we reveal the reaction mechanism of the catalytic residue-mutated glycosidase that shows the high transfer yield; 3) a pore canal at the active site of glycosidase plays a role that supplies the catalytic water by study at the molecular level. 4) we analyze the polysaccharide-producing enzyme and identify its structural elements to contribute to the synthesis of oligosaccharides.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Heterologous production and characterization of catalytic subunit of glucosidase II in Schizosaccharomyces pombe2017
Author(s)
Masayuki Okuyama, Masashi Miyamoto, Takayoshi Tagami, Ichiro Matsuo, Ma Min, Ryo Serizawa, Masanari Tanuma, Haruhide Mori, Atsuo Kimura
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 印刷中
Related Report
Peer Reviewed / Open Access
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[Journal Article] Kinetic properties and substrate inhibition of α-galactosidase from Aspergillus niger2016
Author(s)
Julan Liao, Masayuki Okuyama, Keigo Ishihara, Yoshinori Yamori, Shigeo Iki, Takayoshi Tagami, Haruhide Mori, Seiya Chiaba, Atsuo Kimura
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 80
Issue: 9
Pages: 1747-1752
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining α-galactosidase (BtGH97b)2016
Author(s)
Masayuki Okuyama, Kana Matsunaga, Ken-ichi Watanabe, Keitaro Yamashita, Takayoshi Tagami, Asako Kikuchi, Min Ma, Patcharapa Klahan, Haruhide Mori, Min Yao, Atsuo Kimura
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Journal Title
FEBS Journal
Volume: 284
Issue: 5
Pages: 766-783
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] Molecular anatomy of novel megalosaccharide-producing enzymes2014
Author(s)
Yuya Kumagai, Juri Sadahiro, Weeranuch Lang, Young-Min Kim, Michiyo Kanegae, Masayuki Okuyama, Haruhide Mori, Kazumi Funane, Zui Fujimoto, Satoshi Ishizuka, Hiroshi Hara, Doman Kim, Atsuo Kimura
Organizer
2014 International Symposium on Biodiversity Conservation for Food Security and Rural Development
Place of Presentation
Kunming (China)
Year and Date
2014-07-13 – 2014-07-19
Related Report
Invited
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