Development of new aquaculture using designated nuclease
| Project/Area Number |
26292104
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Kyoto University |
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Principal Investigator |
Kinoshita Masato 京都大学, (連合)農学研究科(研究院), 助教 (60263125)
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| Co-Investigator(Kenkyū-buntansha) |
豊田 敦 国立遺伝学研究所, 大学共同利用機関等の部局等, その他 (10267495)
家戸 敬太郎 近畿大学, 付置研究所, 教授 (90330240)
吉浦 康寿 国立研究開発法人水産研究・教育機構, その他部局等, 研究員 (90372052)
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| Research Collaborator |
KISHIMOTO Kenta 京都大学, 大学院農学研究科
Murakami Yu 京都大学, 大学院農学研究科
KATAYAMA Takashi 国立研究開発法人水産研究, 教育機構
WASHIO Yohei 近畿大学, 水産研究所
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Keywords | ゲノム編集 / マダイ / トラフグ / ミオスタチン / CRISPR/Cas9 / CRISPR/Cas / メダカ / 遺伝子破壊 |
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| Outline of Final Research Achievements |
In aquaculture, selective breeding has not developed yet. The selective breeding takes much time to establish a new breed, so it is not adequate for aquaculture where new breeds are required immediately. We tried to develop new breeding method for aquaculture using genome editing technology, CRISPR/Cas9. With red sea bream and tiger puffer, the single guide RNAs were designated on the genome sequence in exon 1 of myostatin gene and microinjected into the one-cell stage eggs which are artificially inseminated. As results, the myostain gene of both fish has successfully disrupted and new breeds with enhanced muscle mass have established.
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Report
(5 results)
Research Products
(26 results)
