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Studies on molecular mechanism of gonadal sex differentiation and its application for aquaculture in Nile tilapia

Research Project

Project/Area Number 26292109
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionHokkaido University

Principal Investigator

IJIRI SHIGEHO  北海道大学, 水産科学研究院, 准教授 (90425421)

Co-Investigator(Kenkyū-buntansha) 小林 亨  静岡県立大学, 食品栄養科学部, 教授 (30221972)
足立 伸次  北海道大学, 水産科学研究院, 教授 (40231930)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywordsティラピア / 性分化 / 性統御 / アロマターゼ / 濾胞刺激ホルモン / gsdf
Outline of Final Research Achievements

This study developed DNA makers which enable to select genetic male Nile tilapia. Using DNA maker, YY females were produced, resulting establishment of a method to produce all YY population. This method enable stable production of all male population as industrial useful level. In the tilapia ovarian differentiation, roles of fsh signal seemed limited and linear signaling through foxl2, cyp19a1a and estrogen production seemed sufficient signal for triggering ovarian differentiation. In the testicular differentiation, gsdf was not suggested to locate below dmrt1 signal. The fact that gsdf seemed not to affect estrogen production pathway may suggest tilapia sex differentiating factor have a role to inhibit estrogen synthesis pathway, which resulted in gsdf and dmrt1 expression that lead to trigger the testicular differentiation.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (14 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] 西南大学/生命科学学院(中国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Expression pattern of foxl2 and dmrt1 in gonad of Amur sturgeon Acipenser schrenckii in relation to sex differentiation2017

    • Author(s)
      Okada Hatsumi、Hagihara Seishi、Yamashita Katsumasa、Ijiri Shigeho、Adachi Shinji
    • Journal Title

      Aquaculture

      Volume: 479 Pages: 712-720

    • DOI

      10.1016/j.aquaculture.2017.07.020

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 17β-HSD type 12-like is responsible for maturation-inducing hormone synthesis during oocyte maturation in masu salmon2017

    • Author(s)
      Ijiri Shigeho, Yasushi Shibata, Nonoha Takezawa, Yukinori Kazeto, Naoki Takatsuka, Erika Kato, Seishi Hagihara, Yuichi Ozaki, Shinji Adachi, Kohei Yamauchi and Yoshitaka Nagahama
    • Journal Title

      Endocrinology

      Volume: 158 Pages: 627-639

    • DOI

      10.1210/en.2016-1349

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Androgen induces gonadal soma-derived factor, Gsdf, in XX gonads correlated to sex-reversal but not Dmrt1 directly, in the teleost fish, northern medaka (Oryzias sakaizumii).2016

    • Author(s)
      Horie Y, Myosho T, Sato T, Sakaizumi M, Hamaguchi S, Kobayashi T.
    • Journal Title

      Molecular and Cellular Endocrinology

      Volume: 436 Pages: 141-149

    • DOI

      10.1016/j.mce.2016.07.022

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gonadal soma-derived factor (gsdf), a TGF-beta superfamily gene, induces testis differentiation in the teleost fish Oreochromis niloticus.2015

    • Author(s)
      Kaneko H, Ijiri S, Kobayashi T, Izumi H, Kuramochi Y, Wang DS, Mizuno S, Nagahama Y.
    • Journal Title

      Molecular and Cellular Endocrinology

      Volume: 415 Pages: 87-99

    • DOI

      10.1016/j.mce.2015.08.008

    • NAID

      120005855943

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] ティラピア、チョウザメ、ウナギの性分化2018

    • Author(s)
      井尻成保
    • Organizer
      平成30年度日本水産学会秋期大会、シンポジウム「魚類の性決定・性分化・性転換 -これまでとこれから-」
    • Related Report
      2017 Annual Research Report
  • [Presentation] 17beta-Hydroxysteroid dehydrogenase type 12 is responsible for maturation-inducing steroid synthesis during oocyte maturation in Nile tilapia2018

    • Author(s)
      Aranyakanont, C., Ijiri S., Hasegawa, Y., Adachi, S.
    • Organizer
      11th International symposium on reproductive physiology of fish. 3-8 June, 2018. Manaus Brazil
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The in vivo effects of estradiol and 11-ketotestosterone on vitellogenin physiology in the shortfinned eel, Anguilla australis2018

    • Author(s)
      Thomson-Laing, G., Damsteegt, E.L., Nagata, J., Ijiri, S., Adachi, S., Todo, T., Hiramatsu, N., Lokman, P.M.
    • Organizer
      11th International symposium on reproductive physiology of fish. 3-8 June, 2018. Manaus Brazil
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Amh, a necessary gene for male differentiation but not sufficient for sex determination in all the Nile tilapia populations?2017

    • Author(s)
      Baroiller JF, Sissao R, Barbarini M, Dugue R, Cochet C, Canonne M, Toguyeni A, Melard R, Ijiri S, D’Cotta H.
    • Organizer
      18th International Congress of Comparative Endocrinology
    • Place of Presentation
      Chateau Lake Louise, Lake Louise, Canada
    • Year and Date
      2017-06-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ニホンウナギの生殖腺性分化に及ぼす低密度および単独飼育の影響2017

    • Author(s)
      稲葉駿、松谷紀明、井尻成保、足立伸次
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Gonadal soma-derived factorはティラピアの精巣分化を誘導する2015

    • Author(s)
      井尻成保、金子裕代、泉ひかり、倉持有希、水野翔太、足立伸次、長濱嘉孝
    • Organizer
      平成27年度日本水産学会秋期大会
    • Place of Presentation
      宮城県仙台市、東北大学
    • Year and Date
      2015-09-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 水産重要魚種、ウナギとチョウザメの増養殖研究におけるNGSの利用2015

    • Author(s)
      井尻成保
    • Organizer
      NGS現場の会 第四回研究会
    • Place of Presentation
      茨城県つくば市、つくば国際会議場
    • Year and Date
      2015-07-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ナイルティラピアにおけるgonadal soma-derived factor(gsdf)の機能解析2014

    • Author(s)
      水野翔太、井尻成保、西澤朋実、足立伸次
    • Organizer
      日本水産学会北海道支部大会
    • Place of Presentation
      北海道・函館市・函館国際水産海洋総合研究センター
    • Year and Date
      2014-12-19 – 2014-12-20
    • Related Report
      2014 Annual Research Report
  • [Book] 魚類学 第14章 生殖2017

    • Author(s)
      矢部衞、桑村哲生
    • Total Pages
      377
    • Publisher
      恒星社厚生閣
    • ISBN
      9784769916109
    • Related Report
      2017 Annual Research Report

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Published: 2014-04-04   Modified: 2019-03-29  

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