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Molecular mechanism of novel lectin functions and its application to fish biodefense

Research Project

Project/Area Number 26292111
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionTohoku University

Principal Investigator

Muramoto Koji  東北大学, 生命科学研究科, 名誉教授 (90157800)

Co-Investigator(Kenkyū-buntansha) 永沼 孝子  東北生活文化大学短期大学部, 生活文化学科, 講師 (50250733)
小川 智久  東北大学, 生命科学研究科, 准教授 (80240901)
Co-Investigator(Renkei-kenkyūsha) KAZUMURA Kimiko  浜松ホトニクス株式会社, 中央研究所, 研究員 (20394009)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywordsレクチン / ゼブラフィッシュ / 抗酸化活性 / 生体防御 / 糖鎖認識 / 腸管機能 / 免疫賦活 / 抗酸化ペプチド / ラムノース / ポリアミン / 生化学 / 腸管輸送 / タイトジャンクション
Outline of Final Research Achievements

Novel rhamnose-binding lectins (RBLs), named DRLs, were isolated from the zebrafish eggs. Multiple DRLs were expressed in early embryos. DRLs were most highly expressed in ovary, but they were also distributed in various tissues in male and female fish. They had bacterial agglutination activity. These results suggest that DRLs are involved in innate immunity as a pattern recognition molecule against pathogens. An oxidative stress model was established by treating zebrafish embryos with various kinds of oxidative stress reagents. The oxidative stress was evaluated by measuring reactive oxygen species (ROS) generated in the embryos and intracellular Ca2+ concentration using a newly developed a simultaneous measuring instrument of fluorescence and chemiluminescence. Histidine-containing peptides and polyamines showed ROS scavenging activity in a concentration-dependent manner for any oxidation stress reagents. The morphological change due to UV irradiation was suppressed by spermine.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(22 results)

All 2017 2016 2015 2014

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 5 results,  Open Access: 2 results) Presentation (16 results) Book (1 results)

  • [Journal Article] Lectins as Bioactive Proteins in Foods and Feeds2017

    • Author(s)
      Koji Muramoto
    • Journal Title

      Food Sci. Technol. Res.,

      Volume: 23

    • NAID

      130006069503

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Isolation and Biochemical Characterization of Mucus Proteins in Japanese Bunching Onion (<i>Allium fistulosum</i>) Green Leaves2016

    • Author(s)
      Takashi Yamazaki, Tomohisa Ogawa, Koji Muramoto, Jun Nakahigashi, Atsuko Takeuchi and Hiroshi Ueda
    • Journal Title

      FSTR

      Volume: 22 Issue: 2 Pages: 235-243

    • DOI

      10.3136/fstr.22.235

    • NAID

      130005147061

    • ISSN
      1344-6606, 1881-3984
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Antioxidant Properties of Tripeptides Revealed by a Comparison of Six Different Assays2015

    • Author(s)
      Yumi Ohashi, Ryo Onuma, Takako Naganuma, Tomohisa Ogawa, Ryno Naude, Kiyoshi Nokihara and Koji Muramoto
    • Journal Title

      FSTR

      Volume: 21 Issue: 5 Pages: 695-704

    • DOI

      10.3136/fstr.21.695

    • NAID

      130005106156

    • ISSN
      1344-6606, 1881-3984
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effect of chum salmon egg lectin on tight junctions in Caco-2 cell monolayers.2015

    • Author(s)
      Ryo Nemoto, Shintaro Yamamoto, Tomohisa Ogawa, Ryno Naude and Koji Muramoto
    • Journal Title

      Molecules

      Volume: 20 Pages: 8094-8106

    • DOI

      10.3390/molecules20058094

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Isolation and Biochemical Characterization of Apios Tuber Lectin2015

    • Author(s)
      Eri Kenmochi, Syed Rashel Kabir, Tomohisa Ogawa, Ryno Naude, Hiroaki Tateno, Jun Hirabayashi and Koji Muramoto
    • Journal Title

      Molecules

      Volume: 20 Pages: 987-1002

    • DOI

      10.3390/molecules20010987

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ゼブラフィッシュ胚膜における物質輸送経路2016

    • Author(s)
      村本光二、大山結香、下岡千尋、小川智久、鈴木徹、數村公子
    • Organizer
      平成 28 年度日本水産学会秋季大会
    • Place of Presentation
      近畿大学奈良キャンパス(奈良県奈良市)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] ゼブラフィッシュ胚におけるポリアミン類の抗酸化特性2016

    • Author(s)
      下岡千尋、大山結香、小川智久、村本光二、鈴木 徹、數村公子
    • Organizer
      平成 28 年度日本水産学会秋季大会
    • Place of Presentation
      近畿大学奈良キャンパス(奈良県奈良市)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] ココナッツ粉由来β1,4-マンノビオースの抗酸化特性2016

    • Author(s)
      大山結香、下岡千尋、西東恵梨、小川智久、村本光二、福井健介、數村公子
    • Organizer
      日本食品科学工学会第63回大会
    • Place of Presentation
      名城大学天白キャンパス(愛知県名古屋市)
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] ネギ葉身部粘液に含まれる生理活性タンパク質の生化学的性状2016

    • Author(s)
      伴野詢太、山﨑崇、小川智久、村本光二、平山洋佑、竹内敦子、上田浩史
    • Organizer
      日本食品科学工学会第63回大会
    • Place of Presentation
      名城大学天白キャンパス(愛知県名古屋市)
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 抗腫瘍性ルナシンの大豆2Sアルブミンからの生成機構の解明2016

    • Author(s)
      平垣内一子、坪野真由美、佐野まどか、佐藤瑠依、小川智久、村本光二
    • Organizer
      日本食品科学工学会第63回大会
    • Place of Presentation
      名城大学天白キャンパス(愛知県名古屋市)
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 小型魚類における摂餌量測定と摂餌誘引評価への応用2016

    • Author(s)
      笹渕和樹,菅原沙恵子,小川智久,村本光二
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゼブラフィッシュにおける酸化ストレス応答とヒスチジン含有ペプチドの抗酸化特性2016

    • Author(s)
      下岡千尋,大山結香,小川智久,村本光二,鈴木徹,數村公子
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 蛍光マーカーを用いたゼブラフィッシュ胚の輸送機構解析2015

    • Author(s)
      大山結香,後藤優佳,小川智久,村本光二
    • Organizer
      日本農芸化学会東北支部第150回大会
    • Place of Presentation
      東北大学農学研究科(宮城県仙台市)
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゼブラフィッシュにおける酸化ストレス応答と抗酸化ペプチドの機能特性2015

    • Author(s)
      下岡千尋,後藤優佳,大山結香,小川智久,村本光二,鈴木徹
    • Organizer
      平成27年度日本水産学会秋季大会
    • Place of Presentation
      東北大学川内キャンパス(宮城県仙台市)
    • Year and Date
      2015-09-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 水産魚介類由来抗酸化ペプチドの構造・活性相関2015

    • Author(s)
      大沼諒,大橋由美,永沼孝子,小川智久,村本光二
    • Organizer
      平成27年度日本水産学会秋季大会
    • Place of Presentation
      東北大学川内キャンパス(宮城県仙台市)
    • Year and Date
      2015-09-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] シロサケ卵レクチンがヒトCaco-2細胞に及ぼす影響2015

    • Author(s)
      根本諒,永沼孝子,小川智久,村本光二
    • Organizer
      平成27年度日本水産学会秋季大会
    • Place of Presentation
      東北大学川内キャンパス(宮城県仙台市)
    • Year and Date
      2015-09-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Caco-2細胞単層膜におけるタイトジャンクションにおよぼすレクチンの影響2015

    • Author(s)
      根本諒,永沼孝子,小川智久,村本光二
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 蛍光マーカーを用いたゼブラフィッシュ胚の輸送機構解析2015

    • Author(s)
      大山結香,後藤優佳,小川智久,村本光二
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゼブラフィッシュにおける植物性タンパク質の摂取と吸収2014

    • Author(s)
      菅原沙恵子,齋藤信裕,永沼孝子,小川智久,村本光二
    • Organizer
      平成26年度日本農芸化学会北海道・東北合同支部大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-09-22 – 2014-09-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] ペプチドの抗酸化活性評価のための測定法の比較2014

    • Author(s)
      大沼諒,大橋由美,永沼孝子,小川智久,村本光二
    • Organizer
      日本食品科学工学会第61回大会
    • Place of Presentation
      中村学園大学
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] ネギ葉身部粘液に含まれるタンパク質成分の分析2014

    • Author(s)
      山崎崇,永沼孝子,小川智久,村本光二,上田浩史
    • Organizer
      日本食品科学工学会第61回大会
    • Place of Presentation
      中村学園大学
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Book] 日本食およびその素材の健康機能性開発2016

    • Author(s)
      村本光二
    • Publisher
      シーエムシー出版
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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