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The establishment of cattle production using freeze-dried somatic cells and spematozoa

Research Project

Project/Area Number 26292140
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Animal production science
Research InstitutionKochi University

Principal Investigator

MATSUKAWA KAZUTSUGU  高知大学, その他の研究科, 准教授 (00532160)

Co-Investigator(Kenkyū-buntansha) 市川 明彦  名城大学, 理工学部, 准教授 (20377823)
赤木 悟史  国立研究開発法人農業・食品産業技術総合研究機構, その他部局等, 研究員 (70414696)
及川 俊徳  宮城県畜産試験場, その他部局等, 研究員 (70588962)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywordsウシ / 凍結乾燥保存 / 体細胞 / 精子 / フリーズドライ保存 / フリーズドライ / 核移植 / フリーズドライ細胞 / フリーズドライ精子 / 顕微授精
Outline of Final Research Achievements

In general, mammalian somatic cells are preserved in the ultra low temperature freezer or the liquid nitrogen. However, the maintenance and transportation of frozen cells are costly, and there are problems with a safety aspect on liquid nitrogen. Freeze drying, which removes moisture through sublimation, has been used for a long-term storage of food, drug, yeast. To our knowledge, little work has been devoted to freeze dry for mammalian somatic cells. The objective of this study was to establish of cattle production using freeze-dried somatic cells and spematozoa, Our results suggest that bovine freeze-dried fibroblast cells would be useful as a donor cell for nuclear transfer, because they preserve their nuclear structure at -30℃. And the DNA damage in bovine spermatozoa are suppressed after freeze-drying, and freeze-dried spermatozoa produce blastocysts after ICSI. However, calves could not produced using blastocysts derived from freeze-dried somatic cells and spermatozoa.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(10 results)

All 2016 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Factors affecting the development of somatic cell nuclear transfer embryos in cattle2014

    • Author(s)
      Satoshi Akagi, Kazutsugu Matsukawa, Seiya Takahashi
    • Journal Title

      Journal of Reproduction and Development

      Volume: 60 Pages: 329-335

    • NAID

      130004700241

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 凍結乾燥によるウシ体細胞の保存とその応用2016

    • Author(s)
      松川 和嗣, 本郷 新, 田村 慎之介, 枝重 圭祐
    • Organizer
      Cryopreservation Conference 2016
    • Place of Presentation
      岡崎コンファレンスセンター (愛知県岡崎市)
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Characteristics of bovine fibroblast cells after freeze drying2016

    • Author(s)
      Shin Hongo, Shinnosuke Tamura, Satoshi Akagi, Akihiko Ichikawa, Toshinori Oikawa, Keisuke Edashige, Kazutsugu Matsukawa
    • Organizer
      17th AAAP Animal Science Congress
    • Place of Presentation
      九州産業大学 (福岡県福岡市)
    • Year and Date
      2016-08-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Factors affecting DNA damage in bovine spermatozoa after freeze drying2016

    • Author(s)
      Kazutsugu Matsukawa, Mana Nitta, Toshinori Oikawa,Shinouke Tamura, Mami Hosokawa, Aoi Kono, Satoshi Akagi, Akihiko Ichikawa, Keisuke Edashige
    • Organizer
      17th AAAP Animal Science Congress
    • Place of Presentation
      九州産業大学 (福岡県福岡市)
    • Year and Date
      2016-08-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Trial of nuclear transfer from bovine freeze-dried fibroblast cells2016

    • Author(s)
      Shinnosuke Tamura, Satoshi Akagi, Shin Hongo, Akihiko Ichikawa3, Toshinori Oikawa, Keisuke Edashige, Pasqualino Loi, Kazutsugu Matsukawa
    • Organizer
      17th AAAP Animal Science Congress
    • Place of Presentation
      九州産業大学 (福岡県福岡市)
    • Year and Date
      2016-08-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 長期保存フリーズドライ体細胞を用いたウシ核移植胚の作出2015

    • Author(s)
      田村慎之介
    • Organizer
      日本畜産学会第120回大会
    • Place of Presentation
      酪農学園大学
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 凍結乾燥したウシ線維芽細胞の特性および核移植後の発生能2014

    • Author(s)
      尾崎 耕、郡 七海、竹中 由布、枝重 圭祐、葛西 孫三郎、松川 和嗣
    • Organizer
      日本繁殖生物学会
    • Place of Presentation
      帯広畜産大学
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Book] 最新畜産ハンドブック2014

    • Author(s)
      扇元 敬二 (編集)、松川 和嗣 (分担著者)
    • Total Pages
      768
    • Publisher
      講談社
    • Related Report
      2014 Annual Research Report
  • [Book] Transgenic Animal Technology 3rd Edition2014

    • Author(s)
      Pinkert CA (編集)、Akagi Satoshi, Matsukawa Kazutsugu (分担著者)
    • Total Pages
      714
    • Publisher
      Elsevior
    • Related Report
      2014 Annual Research Report
  • [Remarks] 褐毛和種生産振興ネットワーク

    • URL

      http://akaushi-net.jp/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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