Elucidation of embryonic secretory factors using bovine endometrial spheroids
| Project/Area Number |
26292141
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Kyushu University |
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Principal Investigator |
Yamauchi Nobuhiko 九州大学, (連合)農学研究科(研究院), 准教授 (00363325)
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| Co-Investigator(Kenkyū-buntansha) |
川原 学 北海道大学, (連合)農学研究科(研究院), 准教授 (70468700)
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| Research Collaborator |
Md. Rashedul Islam
Mohamed El-Sharawy
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | ウシ / 子宮内膜 / スフェロイド / 胚性分泌因子 / RNAseq / LC/MS/MS / 胚性シグナル応答遺伝子 / 着床 |
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| Outline of Final Research Achievements |
The present study aimed to analyze genes whose expression is induced by embryos and to identify embryonic secretory factors that induce gene expression in cattle. Analysis of the embryonic secretory factor of bovine elongated embryo by LC / MS / MS resulted in the identification of 6 cytokines and 2 growth factors. Furthermore, from the results obtained by RNAseq analysis, upstream factors were analyzed for genes whose expression increased in the endometrium of the implantation period. As a result, in addition to IFNT, genes considered to be controlled by MIF was included. These results suggested that MIF might control endometrial function during implantation as an embryonic secretory factor other than IFNT.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Development of an in vitro model for the analysis of bovine endometrium using simple techniques.2015
Author(s)
Yamauchi K, Yamauchi N, Yamagami K, Nakamura N, Yamashita S, Islam MR, Tabata S, Yahiro K, Tamura T, Hashizume K, Hattori MA.
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Journal Title
Animal Science Journal
Volume: 86
Pages: 1-6
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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