A trial to overcome the injury caused by chilling/heat stress in oocytes/embryos by controlling temperature-sensitive channel activities
| Project/Area Number |
26292170
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高橋 昌志 北海道大学, 農学研究院, 教授 (10343964)
越本 知大 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
松川 和嗣 高知大学, 教育研究部総合科学系生命環境医学部門, 准教授 (00532160)
葛西 孫三郎 高知大学, 教育研究部総合科学系, 教授 (60152617)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Keywords | 低温傷害 / TRPチャンネル / ブタ卵子 / ブタ胚 / ウシ胚 / 高温傷害 |
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| Outline of Final Research Achievements |
In this study, we examined whether a low-temperature sensitive transient receptor potential (TRP) channel was involved in the chilling injury of pig oocytes/embryos. The mRNA of TRPA1, which is sensitive to the temperature at lower than 17oC, was expressed in pig oocytes and embryos at various stages. Exposure of oocytes/embryos to 15oC for 15 min markedly decreased their viability. Exposure of oocytes to 15oC increased intracellular Ca2+ immediately. The pretreatment of oocytes with AP-18, a specific inhibitor for TRPA1, suppressed the increase in their intracellular Ca2+ and the decrease in their viability by the exposure to 15oC. These results indicate that TRPA1 is involved in the chilling injury of pig oocytes/embryos, and that the injury can be circumvented by suppressing TRPA1 activity. Our findings would be useful for developing a cryopreservation method for pig oocytes/embryos.
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage2014
Author(s)
Atsushi Yamashita, Yan Zhao, Yunosuke Matsuura, Kazuaki Yamasaki, Sayaka Moriguchi-Goto, Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Songji Zhao, Yuji Kuge, Yujiro Asada
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Susceptibility of muridae cell lines to ecotropic murine leukemia virus and the cationic amino acid transporter 1 viral receptor sequences: implications for evolution of the viral receptor.2014
Author(s)
Kakoki K, Shinohara A, Izumida M, Koizumi Y, Honda E, Kato G, Igawa T, Sakai H, Hayashi H, Matsuyama T, Morita T, Koshimoto C, Kubo Y.
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Journal Title
Virus Genes
Volume: 48
Pages: 448-456
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm2014
Author(s)
Kazutoshi Nishijima, Shinji Yamaguchi, Mai Tanaka, Yusuke Sakai, Chihiro Koshimoto, Masatoshi Morimoto, TeruoWatanabe, Jianglin Fan, Shuji Kitajima
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Journal Title
Exp. Anim.
Volume: 63
Issue: 2
Pages: 149-154
DOI
NAID
ISSN
0007-5124, 1341-1357, 1881-7122
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Molecular phylogeny of East and Southeast Asian fossorial moles (Eulipotyphla, Talpidae).2014
Author(s)
Shinohara, A., Kawada, S., Son, N. T., Koshimoto, C., Endo, H., Can, D. N. and Suzuki, H.
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Journal Title
J. Mamalogy
Volume: 95
Pages: 455-466
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Cold-sensitive ion channels are involved in chilling injury of pig oocytes.2016
Author(s)
Keisuke Edashige, Yuki Iwahara, Shota Nariai, Yuko Nishiya, Mizuho Kitayama, Saori Niimi, Shinsuke Seki, Chihiro Koshimoto, Kazutsugu Matsukawa, Magosaburo Kasai
Organizer
53rd Annual Meeting of the Society for Cryobiology
Place of Presentation
Fairmont Chateau Laurier, Ottawa, Ontario, Canada
Year and Date
2016-07-23
Related Report
Int'l Joint Research
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