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A trial to overcome the injury caused by chilling/heat stress in oocytes/embryos by controlling temperature-sensitive channel activities

Research Project

Project/Area Number 26292170
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Integrative animal science
Research InstitutionKochi University

Principal Investigator

EDASHIGE Keisuke  高知大学, 教育研究部総合科学系, 教授 (30175228)

Co-Investigator(Kenkyū-buntansha) 高橋 昌志  北海道大学, 農学研究院, 教授 (10343964)
越本 知大  宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
松川 和嗣  高知大学, 教育研究部総合科学系生命環境医学部門, 准教授 (00532160)
葛西 孫三郎  高知大学, 教育研究部総合科学系, 教授 (60152617)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywords低温傷害 / TRPチャンネル / ブタ卵子 / ブタ胚 / ウシ胚 / 高温傷害
Outline of Final Research Achievements

In this study, we examined whether a low-temperature sensitive transient receptor potential (TRP) channel was involved in the chilling injury of pig oocytes/embryos. The mRNA of TRPA1, which is sensitive to the temperature at lower than 17oC, was expressed in pig oocytes and embryos at various stages. Exposure of oocytes/embryos to 15oC for 15 min markedly decreased their viability. Exposure of oocytes to 15oC increased intracellular Ca2+ immediately. The pretreatment of oocytes with AP-18, a specific inhibitor for TRPA1, suppressed the increase in their intracellular Ca2+ and the decrease in their viability by the exposure to 15oC. These results indicate that TRPA1 is involved in the chilling injury of pig oocytes/embryos, and that the injury can be circumvented by suppressing TRPA1 activity. Our findings would be useful for developing a cryopreservation method for pig oocytes/embryos.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (55 results)

All 2016 2015 2014

All Journal Article (19 results) (of which Peer Reviewed: 18 results,  Open Access: 11 results,  Acknowledgement Compliant: 3 results) Presentation (32 results) (of which Int'l Joint Research: 5 results,  Invited: 6 results) Book (4 results)

  • [Journal Article] The movement of water and cryoprotectants across the plasma membrane of mammalian oocytes and embryos and its relevance to vitrification2016

    • Author(s)
      Keisuke Edashige
    • Journal Title

      Journal of Reproduction and Development

      Volume: 62 Issue: 4 Pages: 317-321

    • DOI

      10.1262/jrd.2016-048

    • NAID

      130005406816

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial diversity in forestomach and caecum contents of thegreater long-tailed hamster Tscherskia triton (Rodentia: Cricetidae).2016

    • Author(s)
      Akio Shinohara, Eita Uchida, Hiroki Shichijo, Shinsuke H. Sakamoto, Tetsuo Morita, Chihiro Koshimoto
    • Journal Title

      Mammalian Biology

      Volume: 81 Issue: 1 Pages: 46-52

    • DOI

      10.1016/j.mambio.2014.10.007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] N, N-Dimethylglycine decreases oxidative stress and improves <i>in vitro</i> development of bovine embryos2016

    • Author(s)
      Toshikiyo Takahashi, Kouya Sasaki, Tamas Somfai, Takashi Nagai, Noboru Manabe, Keisuke Edashige
    • Journal Title

      Journal of Reproduction and Development

      Volume: 62 Issue: 2 Pages: 209-212

    • DOI

      10.1262/jrd.2015-149

    • NAID

      130005146469

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Trap distance affects the efficiency and robustness in monitoring the abundance and composition of forest-floor rodents2015

    • Author(s)
      Sakamoto SH, Suzuki SN, Koshimoto C, Okubo Y, Eto T, Suzuki RO.
    • Journal Title

      Journal of Forest Research

      Volume: 20 Issue: 1 Pages: 151-159

    • DOI

      10.1007/s10310-014-0447-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Generation of aminoterminally truncated, stable types of bioactive bovine and porcine fibroblast growth factor 4 in Escherichia coli.2015

    • Author(s)
      Sugawara S., Ito T., Sato Shiori, Sato Yuki, Sasaki A., Fukuda T., Yamanaka K., Sakatani M., Takahashi M. and Kobayashi M.
    • Journal Title

      Biotechnology and Applied Biochemistry

      Volume: 62 Issue: 2 Pages: 164-172

    • DOI

      10.1002/bab.1251

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Heat stress during in vitro fertilization decreases fertilization success by disrupting anti-polyspermy systems of the oocytes2015

    • Author(s)
      Sakatani M, Yamanaka K, Balboula AZ, Takenouchi N, Takahashi M
    • Journal Title

      Molecular Reproduction and Development

      Volume: 82 Issue: 1 Pages: 36-47

    • DOI

      10.1002/mrd.22441

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Magnitude of food overabundance affects expression of daily torpor.2015

    • Author(s)
      T. Eto, R. Hayashi, Y. Okubo, A. Kashimura, C. Koshimoto, S.H. Sakamoto, and T. Morita.
    • Journal Title

      Physiol. Behav.

      Volume: 139 Pages: 519-523

    • DOI

      10.1016/j.physbeh.2014.12.007

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of mixed minerals by observing intestinal epithelial cell alterations in piglets.2014

    • Author(s)
      C. Maneewan, A. Mekbungwan, K. Yamauchi, and K. Edashige.
    • Journal Title

      Am. J. Anim. Vet. Sci.

      Volume: 9 Issue: 2 Pages: 137-143

    • DOI

      10.3844/ajavsp.2014.137.143

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage2014

    • Author(s)
      Atsushi Yamashita, Yan Zhao, Yunosuke Matsuura, Kazuaki Yamasaki, Sayaka Moriguchi-Goto, Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Songji Zhao, Yuji Kuge, Yujiro Asada
    • Journal Title

      PLos One

      Volume: 9 Issue: 1 Pages: e86426-e86426

    • DOI

      10.1371/journal.pone.0086426

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Susceptibility of muridae cell lines to ecotropic murine leukemia virus and the cationic amino acid transporter 1 viral receptor sequences: implications for evolution of the viral receptor.2014

    • Author(s)
      Kakoki K, Shinohara A, Izumida M, Koizumi Y, Honda E, Kato G, Igawa T, Sakai H, Hayashi H, Matsuyama T, Morita T, Koshimoto C, Kubo Y.
    • Journal Title

      Virus Genes.

      Volume: 48 Issue: 3 Pages: 448-456

    • DOI

      10.1007/s11262-014-1036-1

    • NAID

      120006986554

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm2014

    • Author(s)
      Kazutoshi Nishijima, Shinji Yamaguchi, Mai Tanaka, Yusuke Sakai, Chihiro Koshimoto, Masatoshi Morimoto, TeruoWatanabe, Jianglin Fan, Shuji Kitajima
    • Journal Title

      Experimental Animals

      Volume: 63 Issue: 2 Pages: 149-154

    • DOI

      10.1538/expanim.63.149

    • NAID

      130003391615

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular phylogeny of East and Southeast Asian fossorial moles (Eulipotyphla, Talpidae).2014

    • Author(s)
      Shinohara, A., Kawada, S., Son, N. T., Koshimoto, C., Endo, H., Can, D. N. and Suzuki, H.
    • Journal Title

      Journal of Mammalogy

      Volume: 95 Issue: 3 Pages: 455-466

    • DOI

      10.1644/13-mamm-a-135

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Huddling facilitates expression of daily torpor in the large Japanese field mouse Apodemus speciosus.2014

    • Author(s)
      T. Eto, S. H. Sakamoto, Y. Okubo, C. Koshimoto, A. Kashimura, and T. Morita.
    • Journal Title

      Physiol. Behav.

      Volume: 133 Pages: 22-29

    • DOI

      10.1016/j.physbeh.2014.04.051

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of type III antifreeze protein on sperm and embryo cryopreservation in rabbit2014

    • Author(s)
      Kazutoshi Nishijima, Mai Tanaka, Yusuke Sakai, Chihiro Koshimoto, Masatoshi Morimoto, TeruoWatanabe, Jianglin Fan, Shuji Kitajima
    • Journal Title

      Cryobiology

      Volume: (in press) Issue: 1 Pages: 22-25

    • DOI

      10.1016/j.cryobiol.2014.04.014

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of human serum albumin on the bile acid-mediated inhibition of liver microsomal type 1 11β-hydroxysteroid dehydrogenase.2014

    • Author(s)
      Y. Maeda, M. Funagayama, A. Shinohara, C. Koshimoto, H. Furusawa, H. Nakahara, Y. Yamaguchi, T. Saitoh, T. Yamamoto, and K. Komaki.
    • Journal Title

      J. Physiol. Biochem.

      Volume: 70 Issue: 3 Pages: 849-855

    • DOI

      10.1007/s13105-014-0353-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression and regulation of Foxa2 in the rat uterus during early pregnancy2014

    • Author(s)
      Yamagami K, Yamauchi N, Kubota K, Nishimura S, Chowdhury VS, Yamanaka K, Takahashi M, Tabata S, and Hattori MA.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 60 Issue: 6 Pages: 468-475

    • DOI

      10.1262/jrd.2014-086

    • NAID

      130004690752

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of Aldo-keto Reductase 1C23 in the Equine Corpus Luteum in Different Luteal Phases2014

    • Author(s)
      K. Kozai, T. Hojo, S. Tokuyama, A.Z. Szostek, M. Takahashi, M. Sakatani, Y. Nambo, D.J. Skarzynski, K. Okuda.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 60 Issue: 2 Pages: 150-154

    • DOI

      10.1262/jrd.2013-120

    • NAID

      130003390823

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biological Activity of Recombinant Bovine Interferon τ Produced by a Silkworm-Baculovirus Gene Expression System2014

    • Author(s)
      H. Takahashi, M. Tsunazaki, T. Hamano, M. Takahashi, K. Okuda, S. Inumaru, A. Okano, M. Geshi, M. Hirako.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 76 Issue: 3 Pages: 447-451

    • DOI

      10.1292/jvms.12-0403

    • NAID

      130003382378

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Factors Affecting the Development of Somatic Cell Nuclear Transfer Embryos in Cattle2014

    • Author(s)
      S. Akagi, K. Matsukawa, and S. Takahashi.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 60 Issue: 5 Pages: 329-335

    • DOI

      10.1262/jrd.2014-057

    • NAID

      130004700241

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2014 Annual Research Report
    • Open Access
  • [Presentation] Influence of captivity oncecum microbial diversity in the large Japanese field mouse (Apodemuss peciosus).2016

    • Author(s)
      Akio Shinohara, Hikari Inoue, Yoshitoshi Ogura, Makoto Nohara, Shinsuke H. Sakamoto, Tetsuya Hayashi, Chihiro Koshimoto
    • Organizer
      Joint meeting of the 22nd International Congress of Zoology(ICZ) & the 87th Meeting of the ZSJ.
    • Place of Presentation
      Okinawa Institute of Science and Technology Graduate University, Kunigami, Okinawa
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gut microbiota of Asian musk shrew (Suncus murinus) revealed by 16S rRNA gene sequences.2016

    • Author(s)
      Makoto Nohara, Shinsuke H. Sakamoto, Takamichi Jogahara, Atsushi Kashimura, Chihiro Koshimoto, Akio Shinohara.
    • Organizer
      Joint meeting of the 22nd International Congress of Zoology(ICZ) & the 87th Meeting of the ZSJ.
    • Place of Presentation
      Okinawa Institute of Science and Technology Graduate University, Kunigami, Okinawa
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 浸透圧が極めて高い保存液を用いた卵巣のガラス化凍結保存2016

    • Author(s)
      枝重圭祐
    • Organizer
      Cryopreservation Conference 2016
    • Place of Presentation
      愛知県岡崎市岡崎コンファレンスセンター
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 凍結乾燥によるウシ体細胞の保存とその応用2016

    • Author(s)
      松川和嗣、本郷新、田村慎之介、枝重圭祐
    • Organizer
      Cryopreservation Conference 2016
    • Place of Presentation
      愛知県岡崎市岡崎コンファレンスセンター
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 平衡ガラス化法によるマウス卵巣の凍結保存のこころみ2016

    • Author(s)
      岩原悠樹、横堀誠也、倍味那々子、本田宙、越本知大、松川和嗣、葛西孫三郎、枝重圭祐
    • Organizer
      第109回 日本繁殖生物学会相模原大会
    • Place of Presentation
      神奈川県相模原市中央区麻布大学8号館
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] L-アスコルビン酸2リン酸の培地への添加がウシ体外受精胚の割球分離後の発生に及ぼす影響2016

    • Author(s)
      田村慎之介,細川真実、河野葵、枝重圭祐、松川和嗣
    • Organizer
      第109回 日本繁殖生物学会相模原大会
    • Place of Presentation
      神奈川県相模原市中央区麻布大学8号館
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] The effect of knock down of IFNAR by RNAi on expression of interferon-τ induced genes in bovine endometrial epithelial cells.2016

    • Author(s)
      Takahiro Shirozu, Toshiyuki Suzuki, Hiroki Iwano, Takatoshi Ogiso, Hanako Bai, Manabu Kawahara, Koji Kimura, Masashi Takahashi
    • Organizer
      The 17th Asian-Australian Association of Animal Production Societies Animal Science Congress
    • Place of Presentation
      Kyushu Sangyo University, Higashi-ku, Fukuoka
    • Year and Date
      2016-08-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of cysteine protease on the quality and developmental competence of bovine oocyte.2016

    • Author(s)
      Masashi Takahashi
    • Organizer
      The 17th Asian-Australian Association of Animal Production Societies Animal Science Congress
    • Place of Presentation
      Kyushu Sangyo University, Higashi-ku, Fukuoka
    • Year and Date
      2016-08-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cold-sensitive ion channels are involved in chilling injury of pig oocytes.2016

    • Author(s)
      Keisuke Edashige, Yuki Iwahara, Shota Nariai, Yuko Nishiya, Mizuho Kitayama, Saori Niimi, Shinsuke Seki, Chihiro Koshimoto, Kazutsugu Matsukawa, Magosaburo Kasai
    • Organizer
      53rd Annual Meeting of the Society for Cryobiology
    • Place of Presentation
      Fairmont Chateau Laurier, Ottawa, Ontario, Canada
    • Year and Date
      2016-07-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Equilibrium vitrification, a new novel cryopreservation method2015

    • Author(s)
      Keisuke Edashige
    • Organizer
      第42回日本低温医学会総会
    • Place of Presentation
      ホテル日航金沢金沢アートホール(石川県金沢市)
    • Year and Date
      2015-11-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 平衡ガラス化法によるマウス卵子の凍結保存の試み2015

    • Author(s)
      福嶋和貴, 近藤詩織, 平川猛, 岩原悠樹, 横堀誠也, 越本知大, 松川和嗣, 葛西孫三郎, 枝重圭祐
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎大学木花キャンパス(宮崎県宮崎市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 平衡ガラス化法を用いたゼブラフィッシュ胚のガラス化凍結保存の試み2015

    • Author(s)
      横堀誠也, 竹下純隆, 福嶋和貴, 岩原悠樹, 越本知大, 松川和嗣, 葛西孫三郎, 枝重圭祐
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎大学木花キャンパス(宮崎県宮崎市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 温度感受性TRPチャンネルの哺乳動物卵子における低温傷害への関与2015

    • Author(s)
      岩原悠樹, 北山みずほ, 新見沙織, 福嶋和貴, 横堀誠也, 越本知大, 松川和嗣, 葛西孫三郎, 枝重圭祐
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎大学木花キャンパス(宮崎県宮崎市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 卵子および胚の低温生物学的特性とガラス化凍結保存に関する研究2015

    • Author(s)
      枝重圭祐
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎大学木花キャンパス(宮崎県宮崎市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 発情周期別ウシ子宮内膜におけるTRPチャネルの発現動態2015

    • Author(s)
      岩野弘暉, 小木曽貴季, 白水貴大, 川原学, 枝重圭祐, 高橋昌志
    • Organizer
      日本畜産学会第120回大会
    • Place of Presentation
      酪農学園大学(北海道江別市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] アスコルビン酸2リン酸は暑熱ストレスに暴露されたウシ体外受精胚の作出率を改善する2015

    • Author(s)
      細川真美, 竹中由布, 枝重圭祐, 松川和嗣
    • Organizer
      日本畜産学会第120回大会
    • Place of Presentation
      酪農学園大学(北海道江別市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 長期保存フリーズドライ体細胞を用いたウシ核移植胚の作出2015

    • Author(s)
      田村慎之介, 小西裕子, 枝重圭祐, 赤木悟史, 松川和嗣
    • Organizer
      日本畜産学会第120回大会
    • Place of Presentation
      酪農学園大学(北海道江別市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 発情周期におけるウシ生殖器のアクポリン遺伝子群現動態2015

    • Author(s)
      小木曽 貴季、岩野 弘暉、白水 貴大、川原 学、高橋 昌志
    • Organizer
      日本畜産学会第120回大会
    • Place of Presentation
      酪農学園大学(北海道江別市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガラス化凍結保存後の胚の生存性に関わる要因2015

    • Author(s)
      枝重圭祐
    • Organizer
      第22回日本胚移植研究会
    • Place of Presentation
      高知大学物部キャンパス(高知県南国市)
    • Year and Date
      2015-08-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 平衡ガラス化法による受精卵の凍結保存2015

    • Author(s)
      枝重圭祐
    • Organizer
      第60回低温生物工学会大会
    • Place of Presentation
      東京工科大学八王子キャンパス(八王子市)
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 哺乳動物胚の細胞膜透過性 -凍結保存における重要性-2015

    • Author(s)
      枝重圭祐
    • Organizer
      第60回日本生殖医学会学術講演会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-04-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Long term assessment of phosphorus emission from cattle fattening.2014

    • Author(s)
      H.i Tsutsui, H. Sakaguchi, X. Sun, S. Toda, K. Matsukawa, T. Fujiwara, K. Oshita, and M. Takaoka.
    • Organizer
      9th IWA International Symposium on Waste Management Problems in Agto-Industries
    • Place of Presentation
      The Crown Palais New Hankyu Kochi, Kochi.
    • Year and Date
      2014-11-24 – 2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effects of Yuzu (Citrus junos) peel on differentiation of bovine mesenchymal stem cells derived from subcutaneous tissue.2014

    • Author(s)
      K. Matsukawa, S. Kasamatsu, S. Nojiri, M. Ishioka, S. Nanba, H. Muramatsu, S. Nagata.
    • Organizer
      9th IWA International Symposium on Waste Management Problems in Agto-Industries.
    • Place of Presentation
      The Crown Palais New Hankyu Kochi, Kochi.
    • Year and Date
      2014-11-24 – 2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 野生アカネズミの盲腸内微生物は飼育により変化する.2014

    • Author(s)
      井上比加理,酒井悠輔,坂本信介,越本知大,篠原明男.
    • Organizer
      日本哺乳類学会2014年度大会
    • Place of Presentation
      京都大学吉田キャンパス,京都市.
    • Year and Date
      2014-09-04 – 2014-09-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ゼブラフィッシュ卵子における高浸透圧による傷害メカニズム.2014

    • Author(s)
      福嶋和貴,竹下純隆,濱崎桃子,越本知大,松川和嗣,葛西孫三郎,枝重圭祐.
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広畜産大学,帯広市.
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 温度感受性TRPチャンネルのブタ胚における低温傷害への関与.2014

    • Author(s)
      新見沙織,北山みずほ,菊地和弘,越本知大,松川和嗣,葛西孫三郎,枝重圭祐.
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広畜産大学,帯広市.
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 凍結乾燥したウシ線維芽細胞の特性および核移植後の発生能.2014

    • Author(s)
      尾崎耕,郡七海,竹中由布,枝重圭祐,葛西孫三郎,松川和嗣.
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広畜産大学,帯広市.
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 哺乳動物胚の凍結保存.ワークショップ1:配偶子・胚凍結保存技術の最前線のこれから.2014

    • Author(s)
      枝重圭祐.
    • Organizer
      第32回日本受精着床学会総会
    • Place of Presentation
      ハイアットリージェンシー東京,新宿区.
    • Year and Date
      2014-07-31 – 2014-08-01
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] A trial to improve the tolerance to cryopreservation of zebrafish oocytes by enhancing the expression of aquaporin 3.2014

    • Author(s)
      K. Edashige, M. Hamasaki, M. Takeshita, M. Kitayama, T. Hirakawa, C. Koshimoto, K. Matsukawa, and M. Kasai.
    • Organizer
      SSR2017 Annual Meeting of Society for the Study of Reproduction
    • Place of Presentation
      Grand Rapid, USA.
    • Year and Date
      2014-07-19 – 2014-07-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 水/耐凍剤透過性チャンネルの発現によるゼブラフィッシュ ステージⅢ中期卵子の耐凍性向上の試み.2014

    • Author(s)
      枝重圭祐,濱崎桃子,竹下純隆,北山みずほ,平川猛,越本知大,松川和嗣,葛西孫三郎.
    • Organizer
      第61回日本実験動物学会総会
    • Place of Presentation
      札幌コンベンションセンター,札幌市.
    • Year and Date
      2014-05-15 – 2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヨーロッパモリネズミにおける運動による血中脂質と自発活動量への影響.2014

    • Author(s)
      猪口瑞穂,七條宏樹,坂本信介,篠原明男,越本知大.
    • Organizer
      第61回日本実験動物学会総会
    • Place of Presentation
      札幌コンベンションセンター,札幌市.
    • Year and Date
      2014-05-15 – 2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヨーロッパモリネズミに観察される糖尿病様個体の簡易的な選抜方法の検討.2014

    • Author(s)
      冨山小百合,加藤悟郎,七條宏樹,坂本信介,篠原明男,越本知大.
    • Organizer
      第61回日本実験動物学会総会
    • Place of Presentation
      札幌コンベンションセンター,札幌市.
    • Year and Date
      2014-05-15 – 2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Book] 生殖補助医療(ART)胚培養の理論と実際 日本卵子学会編 第8章生殖細胞の保存 1.凍結理論2016

    • Author(s)
      枝重圭祐
    • Publisher
      近代出版
    • Related Report
      2016 Annual Research Report
  • [Book] 日本のネズミ 多様性と進化 本川雅治編 第7章実験動物としてのアカネズミ-新しい研究資源としての可能性-2016

    • Author(s)
      越本知大
    • Publisher
      東京大学出版
    • Related Report
      2016 Annual Research Report
  • [Book] The movement of water and cryoprotectants in mammalian oocytes and embryos: membrane permeability and aquaporins. In 'Vitrification in assisted reproduction, second edition'2015

    • Author(s)
      Keisuke Edashige, Magosaburo Kasai
    • Publisher
      CRC Press
    • Related Report
      2015 Annual Research Report
  • [Book] Nuclear transfer technology in cattle, sheep, and swine. In "Transgenic Animal Technology 3rd Edition, (C.A. Pinkert ed.)"2014

    • Author(s)
      S. Akagi, K. Matsukawa, and A. Onishi
    • Total Pages
      11
    • Publisher
      Elsevier
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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