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Basic analysis of an organelle-like defensive symbiont found in the Asian citrus psyllid

Research Project

Project/Area Number 26292174
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Insect science
Research InstitutionToyohashi University of Technology

Principal Investigator

NAKABACHI Atsushi  豊橋技術科学大学, エレクトロニクス先端融合研究所, 准教授 (40332267)

Research Collaborator INOUE Hiromitsu  
HIROSE Yu  
Project Period (FY) 2014-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsミカンキジラミ / Profftella / ディアフォリン / 防衛共生 / 細菌叢 / ゲノム / 生物活性 / 抗がん活性 / Profftella armatura / 共生細菌ゲノム / 防衛共生体 / 極小ゲノム / 二次代謝産物 / 薬理活性 / 質量顕微鏡法 / FISH / オルガネラ様防衛共生体 / ポリケチド / 共生細菌叢 / アンプリコン解析
Outline of Final Research Achievements

Profftella, an endosymbiont of the Asian citrus psyllid Diaphorina citri, appears to be an unprecedented organelle-like defensive symbiont. The present study aiming to understand its ecology, function, and evolution revealed the following: (1) bacterial populations of 40 psyllid species and genome sequences of four symbionts including an apparent sister species of Profftella; (2) behavior of Profftella during the transovarial transmission and the development of D. citri; (3) inhibitory effects of diaphorin, a polyketide produced by Profftella, against insects and yeasts; (4) distribution and concentration of diaphorin within D. citri, as well as expression of genes involved in the diaphorin synthesis during the development of D. citri; (5) antitumor activity of diaphorin.

Academic Significance and Societal Importance of the Research Achievements

本研究により、きわめてユニークな「オルガネラ様防衛共生体」の生態を解明し、機能の理解を進め、進化動態の解明に道筋を付けることができた。これらの成果は、生物進化の理解を深化させる学術的意義を持つばかりでなく、世界的に重要な農業害虫の生物学的防除の効率向上や新規防除法開発の基盤を提供するもので、社会的意義も大きい。ディアフォリンについては、抗がん剤シードとしての有望性は低いとの結論を得たが、こうした天然化合物の薬理活性の知見の蓄積は、今後の薬学の発展に資するものである。また、本研究で新たに見出された生物活性は、産業への応用可能性が期待される。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(38 results)

All 2019 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (4 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (25 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results) Book (2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] University of Pittsburgh(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Masaryk University(チェコ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Pittsburgh(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Pittsburgh(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Diaphorin, a polyketide synthesized by an intracellular symbiont of the Asian citrus psyllid, is potentially harmful for biological control agents2019

    • Author(s)
      Yamada Tomoko、Hamada Masato、Floreancig Paul、Nakabachi Atsushi
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 5 Pages: e0216319-e0216319

    • DOI

      10.1371/journal.pone.0216319

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Behavior of bacteriome symbionts during transovarial transmission and development of the Asian citrus psyllid2017

    • Author(s)
      Dan H, Ikeda N, Fujikami M, Nakabachi A
    • Journal Title

      PLoS One

      Volume: 12(12) Issue: 12 Pages: e0189779-e0189779

    • DOI

      10.1371/journal.pone.0189779

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 昆虫のオルガネラ様共生細菌たち2016

    • Author(s)
      中鉢淳
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 54 Issue: 10 Pages: 753-761

    • DOI

      10.1271/kagakutoseibutsu.54.753

    • NAID

      130006083302

    • ISSN
      0453-073X, 1883-6852
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Horizontal gene transfers in insects2015

    • Author(s)
      Nakabachi A
    • Journal Title

      Curr Opin Insect Sci

      Volume: 7 Pages: 24-29

    • DOI

      10.1016/j.cois.2015.03.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Aphid gene of bacterial origin encodes a protein transported to an obligate endosymbiont.2014

    • Author(s)
      Nakabachi A, Ishida K, Hongoh Y, Ohkuma M, Miyagishima SY.
    • Journal Title

      Curr Biol

      Volume: 24 Issue: 14 Pages: R640-R641

    • DOI

      10.1016/j.cub.2014.06.038

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Parallel histories of horizontal gene transfer facilitated extreme reduction of endosymbiont genomes in sap-feeding insects2014

    • Author(s)
      Sloan DB, Nakabachi A, Richards S, Qu J, Murali SC, Gibbs RA, Moran NA
    • Journal Title

      Molecular Biology and Evolution

      Volume: 31(4) Issue: 4 Pages: 857-71

    • DOI

      10.1093/molbev/msu004

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 昆虫-細菌間融合共生系からの有用遺伝子の獲得と応用展開2019

    • Author(s)
      中鉢 淳
    • Organizer
      平成30年度エレクトロニクス先端融合研究所プロジェクト研究成果報告会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Immunochemistry of Dcitri1, an orphan protein highly upregulated in the bacteriome of the Asian citrus psyllid2018

    • Author(s)
      Shogo Hashimoto and Atsushi Nakabachi
    • Organizer
      Irago Conference 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 昆虫-細菌間融合共生系の基盤解析および共生細菌からの有用遺伝子の探索2018

    • Author(s)
      中鉢淳
    • Organizer
      平成29年度エレクトロニクス先端融合研究所プロジェクト研究成果報告会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ミカンキジラミにおけるオルガネラ様共生細菌2種の感染・胚発生時の動態観察2017

    • Author(s)
      池田直也、藤上雅也、壇勲興、中鉢淳
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] ミカンキジラミ共生細菌の産生する新規毒性ポリケチド「ディアフォリン」の局在解析2017

    • Author(s)
      藤上雅也、椛嶋克哉、中鉢淳
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] オルガネラ様共生細菌の産生する新規ポリケチド「ディアフォリン」は真核生物に広く毒性を示す2017

    • Author(s)
      山田倫子、濱田雅東、中鉢淳
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 動物-細菌間融合共生系の基盤解析2017

    • Author(s)
      中鉢淳
    • Organizer
      文部科学省研究大学強化促進事業・豊橋技術科学大学シンポジウム「東三河から世界へ ~産学官連携による新しい価値の創造~」
    • Place of Presentation
      ホテルアソシア豊橋(愛知県豊橋市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 昆虫-細菌間融合共生系の基盤解析および共生細菌からの有用遺伝子の探索2017

    • Author(s)
      中鉢淳
    • Organizer
      平成28年度エレクトロニクス先端融合研究所プロジェクト研究成果報告会
    • Place of Presentation
      豊橋技術科学大学(愛知県豊橋市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] アブラムシ-ブフネラ間共生の理解を目指して2016

    • Author(s)
      中鉢淳
    • Organizer
      日本動物学会第87回沖縄大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Aphid gene of bacterial origin encodes a protein transported to Buchnera2016

    • Author(s)
      Atsushi Nakabachi
    • Organizer
      The XXV International Congress of Entomology
    • Place of Presentation
      Orlando, FL, USA
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] キジラミから見つかったオルガネラ様共生細菌の産生する新規毒性ポリケチド2016

    • Author(s)
      中鉢淳
    • Organizer
      第63回トキシンシンポジウム
    • Place of Presentation
      ほほえみの宿 滝の湯(山形県天童市)
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ミカンキジラミ共生細菌から得られた新規ポリケチド「ディアフォリン」の活性評価2016

    • Author(s)
      山田倫子、濱田雅東、杉野明日香、岡村恵子、中鉢淳
    • Organizer
      日本昆虫学会第76回大会・第60回日本応用動物昆虫学会合同大会
    • Place of Presentation
      大阪府堺市
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 農業害虫から見つかったオルガネラ様防衛共生体2016

    • Author(s)
      中鉢淳
    • Organizer
      第10回日本ゲノム微生物学会年会シンポジウム「微生物の新規機能を探る」
    • Place of Presentation
      東京都目黒区
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] キジラミ共生細菌の産生する新規化合物の生物活性評価等2016

    • Author(s)
      中鉢淳
    • Organizer
      第4回次世代シークエンス技術応用研究会
    • Place of Presentation
      愛知県豊橋市
    • Year and Date
      2016-02-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 昆虫共生細菌からの新規有用化合物の獲得2016

    • Author(s)
      中鉢淳
    • Organizer
      文部科学省研究大学強化促進事業・豊橋技術科学大学シンポジウム「未来への挑戦 ~新たなステージに立つ~」
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2016-01-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] RNA interference approach to analyze functional role of a gene highly expressed in the symbiotic organ of the Asian citrus psyllid, Diaphorina citri2015

    • Author(s)
      Dan H, Nakabachi A
    • Organizer
      The Irago Conference 2015
    • Place of Presentation
      Tahara, Japan
    • Year and Date
      2015-10-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Screening of proteins that potentially play pivotal roles in the aphid-Buchnera symbiosis2015

    • Author(s)
      Onishi K, Ohkuma M, Nakabachi A
    • Organizer
      The Irago Conference 2015
    • Place of Presentation
      Tahara, Japan
    • Year and Date
      2015-10-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Differential toxicity of a novel polyketide diaphorin to various organisms2015

    • Author(s)
      Yamada T, Hamada M, Sugino A, Okamura K, Nakabachi A
    • Organizer
      The Irago Conference 2015
    • Place of Presentation
      Tahara, Japan
    • Year and Date
      2015-10-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNA干渉によるミカンキジラミ共生関連遺伝子の機能解析の試み2015

    • Author(s)
      壇勲興、中鉢淳
    • Organizer
      第30回日本微生物生態学会大会
    • Place of Presentation
      茨城県土浦市
    • Year and Date
      2015-10-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] アブラムシの共生関連機能未知タンパク質と相互作用するタンパク質群の検出2015

    • Author(s)
      尾西桂、大熊盛也、中鉢淳
    • Organizer
      第30回日本微生物生態学会大会
    • Place of Presentation
      茨城県土浦市
    • Year and Date
      2015-10-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 動物界のオルガネラ進化2015

    • Author(s)
      中鉢淳
    • Organizer
      日本進化学会第17回大会ワークショップ「Endosymbiosis and organellogenesis」
    • Place of Presentation
      東京都文京区
    • Year and Date
      2015-08-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 多様なキジラミ系統の共生細菌叢解析2015

    • Author(s)
      中鉢淳、山本昂平、岡村恵子、杉野明日香、井上広光、三澤直美、広瀬侑
    • Organizer
      NGS現場の会 第四回研究会
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2015-07-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] オルガネラ様防衛共生細菌Profftellaの姉妹系統の探索2014

    • Author(s)
      山本昂平、岡村恵子、杉野明日香、三澤直美、広瀬侑、中鉢淳
    • Organizer
      環境微生物系合同大会2014
    • Place of Presentation
      静岡県浜松市
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] RNA干渉によるミカンキジラミ共生関連遺伝子の発現抑制2014

    • Author(s)
      壇勲興、中鉢淳
    • Organizer
      環境微生物系合同大会2014
    • Place of Presentation
      静岡県浜松市
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミカンキジラミ共生細菌から得られた新規ポリケチド「ディアフォリン」の活性評価2014

    • Author(s)
      濱田雅東、杉野明日香、中鉢淳
    • Organizer
      環境微生物系合同大会2014
    • Place of Presentation
      静岡県浜松市
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Book] 半翅目昆虫の菌細胞内共生(「共生の生物学」)2016

    • Author(s)
      中鉢淳
    • Total Pages
      281
    • Publisher
      化学同人
    • Related Report
      2016 Annual Research Report
  • [Book] 農業害虫の生存を支えるオルガネラ様共生細菌(「難培養微生物研究の最新技術Ⅲ」)2015

    • Author(s)
      中鉢淳
    • Total Pages
      10
    • Publisher
      シーエムシー出版
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 細菌細胞肥大化促進剤と細菌細胞の肥大化促進方法2018

    • Inventor(s)
      中鉢淳、山田倫子、青戸淳和、田邊望夢
    • Industrial Property Rights Holder
      中鉢淳、山田倫子、青戸淳和、田邊望夢
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report

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Published: 2014-04-04   Modified: 2023-03-23  

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