| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
We established a technical basis for in vivo analysis of expression patterns and functional analysis of genes regulating embryogenesis in Gryllus bimaculatus, a hemimetabolous insect model system. Using CRISPR/Cas9 system, we developed a gene knock-in technique in the cricket. We applied this technique to knock-in/knockout for efficient identification of knock-out individuals by detecting marker gene expression. In addition, we succeeded in observation of gene expression patterns through developmental stages via marker gene knock-in. Data of expression and function of Hox genes was obtained, including spatial expression patterns in postembryonic stages. Furthermore, we showed availability of our knock-in method to enhancer trap analysis.
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