Study on genomic basis of morphogenesis in hemimetabolous insects using novel genetic modification techniques
| Project/Area Number |
26292176
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
Mito Taro 徳島大学, 生物資源学部, 准教授 (80322254)
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| Co-Investigator(Kenkyū-buntansha) |
友成 さゆり 徳島大学, ソシオテクノサイエンス研究部, 技術専門職員 (40448345)
渡辺 崇人 徳島大学, 農工商連携センター, 特任助教 (30709481)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | ゲノム編集 / 昆虫 / 胚発生 / 後胚発生 / 遺伝子ノックアウト / 形態形成 |
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| Outline of Final Research Achievements |
We established a technical basis for in vivo analysis of expression patterns and functional analysis of genes regulating embryogenesis in Gryllus bimaculatus, a hemimetabolous insect model system. Using CRISPR/Cas9 system, we developed a gene knock-in technique in the cricket. We applied this technique to knock-in/knockout for efficient identification of knock-out individuals by detecting marker gene expression. In addition, we succeeded in observation of gene expression patterns through developmental stages via marker gene knock-in. Data of expression and function of Hox genes was obtained, including spatial expression patterns in postembryonic stages. Furthermore, we showed availability of our knock-in method to enhancer trap analysis.
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Report
(4 results)
Research Products
(28 results)
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[Presentation] Genome sequencing and annotation of the cricket Gryllus bimaculatus, a hemimetabolous insect model2015
Author(s)
Taro Mito, Takehiko Itoh, Hiroya Morimoto, Ray Kajitani, Atsushi Toyoda, Sayuri Tomonari, Masao Fuketa, Takahito Watanabe, Yuji Matsuoka, Sumihare Noji
Organizer
Ninth Annual Arthropod Genomics Symposium
Place of Presentation
米国、カンザス州マンハッタン
Year and Date
2015-06-17
Related Report
Int'l Joint Research
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