Plant stress responses regulated by the circadian clock

• Project/Area Number: 26292191
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Chiba University
• Principal Investigator: HANAOKA Mitsumasa 千葉大学, 大学院園芸学研究科, 准教授 (30508122)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: 概日時計 / ストレス応答 / 遺伝子発現制御 / 葉緑体 / 光合成 / シグマ因子 / SIG5 / ストレス / 環境 / 遺伝子 / 発現制御 / 植物 / 転写制御

Outline of Final Research Achievements

The purpose of this study is to understand molecular mechanisms of plant stress responses regulated by the circadian clock. We performed gene expression as well as phenotypic analyses around dawn in Arabidopsis thaliana, and found that the circadian clock is possible to repress light-dependent stressful condition after dawn. Furthermore, bidirectional signaling between the nucleus and chloroplasts for circadian regulation, and similar stress response mechanisms in photosynthetic microorganisms, have been also characterized.

Research Products

• [Int'l Joint Research] ブリストル大学(イギリス)
• [Int'l Joint Research] ブリストル大学(英国)
• [Int'l Joint Research] ブリストル大学(イギリス)
• [Journal Article] CyanoBase: a large-scale update on its 20th anniversary (2017)
  • Author(s): Fujisawa, T., Narikawa, R., Maeda, S., Watanabe, S., Kanesaki, Y., Kobayashi, K., Nomata, J., Hanaoka, M., Watanabe, M., Ehira, S., Suzuki, E., Awai, K. and Nakamura, Y.
  • Journal Title: Nucleic Acids Res. Volume: 45 Pages: 551-554
  • DOI: 10.1093/nar/gkw1131
  • Peer Reviewed / Open Access
• [Journal Article] Abscisic acid participates in the control of cell-cycle initiation through heme homeostasis in the unicellular red alga Cyanidioschyzon merolae (2016)
  • Author(s): Yuki Kobayashi, Hiroyuki Ando, Mitsumasa Hanaoka, and Kan Tanaka
  • Journal Title: Plant and Cell Physiology Volume: in press Pages: 211-214
  • DOI: 10.1093/pcp/pcw054
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The nuclear-encoded sigma factor SIG4 directly activates transcription of chloroplast psbA and ycf17 genes in the unicellular red alga Cyanidioschyzon merolae (2015)
  • Author(s): Gaku Fujii, Sousuke Imamura, Atsuko Era, Shin-ya Miyagishima, Mitsumasa Hanaoka, and Kan Tanaka
  • Journal Title: FEMS Microbiology Letters Volume: 印刷中
  • DOI: 10.1093/femsle/fnv063
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 共生によるレトログレードシグナリングの成立と進化 (2015)
  • Author(s): 華岡光正
  • Journal Title: 生物科学 Volume: 66 Pages: 208-212
  • Peer Reviewed
• [Journal Article] The circadian regulation of photosynthesis (2014)
  • Author(s): Dodd AN, Kusakina J, Hall A, Gould PD, Hanaoka M.
  • Journal Title: Photosynthesis Research Volume: 119 Pages: 181-190
  • DOI: 10.1007/s11120-013-9811-8
  • Peer Reviewed
• [Journal Article] Sense transgene-induced post-transcriptional gene silencing in tobacco compromises the splicing of endogenous counterpart genes (2014)
  • Author(s): Shin MR, Natsuume M, Matsumoto T, Hanaoka M, Imai M, Iijima K, Oka S, Adachi E, Kodama H.
  • Journal Title: PLoS One Volume: 9
  • DOI: 10.1371/journal.pone.0087869
  • Peer Reviewed
• [Journal Article] Overproduction of stromal ferredoxin:NADPH oxidoreductase in H2O2-accumulating Brassica napus leaf protoplasts (2014)
  • Author(s): Rajesh Tewari, Mamoru Satoh, Sayaka Kado, Kohei Mishina, Misato Anma, Kazuhiko Enami, Mitsumasa Hanaoka and Masami Watanabe
  • Journal Title: Plant Molecular Biology Volume: 86 Pages: 627-639
  • DOI: 10.1007/s11103-014-0252-3
  • Peer Reviewed
• [Presentation] Establishment and evolution of nucleus-chloroplast communication. (2018)
  • Author(s): Mitsumasa Hanaoka
  • Organizer: Microbiology Society Annual Conference 2018
  • Int'l Joint Research / Invited
• [Presentation] Effects of cutting and drying leaves of culinary herbs on contents of bioactive phytochemicals in Perilla frutescens. (2018)
  • Author(s): Natsuko Kagawa, Masahumi Henzan, Hiroya Iguchi and Mitsumasa Hanaoka
  • Organizer: 255th ACS National Meeting and Exposition
  • Int'l Joint Research
• [Presentation] ストレスに応答した青シソ二次代謝関連遺伝子の発現制御 (2018)
  • Author(s): 平安山昌史、井口広也、加川夏子、華岡光正
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] Nuclear gene regulation by retrograde redox signals. (2018)
  • Author(s): Mitsumasa Hanaoka
  • Organizer: International Symposium on Redox dynamics of transcription, translation and protein folding
  • Int'l Joint Research / Invited
• [Presentation] 単細胞紅藻C. merolaeにおける概日時計とオルガネラ機能の相互作用 (2017)
  • Author(s): 片野貴章、雪竹健太郎、高橋広夫、恵良厚子、宮城島進也、今村壮輔、田中寛、華岡光正
  • Organizer: 第16回微生物研究会
• [Presentation] 青シソの二次代謝関連遺伝子発現のストレス応答 (2017)
  • Author(s): 井口広也、平安山昌史、加川夏子、華岡光正
  • Organizer: 日本植物学会第81回大会
• [Presentation] 単細胞紅藻C. merolaeにおける核とオルガネラ間の概日時計情報伝達の解析 (2017)
  • Author(s): 片野貴章、雪竹健太郎、高橋広夫、恵良厚子、宮城島進也、今村壮輔、田中寛、華岡光正
  • Organizer: 日本植物学会第81回大会
• [Presentation] 概日時計に依存して発現するSIG5の葉緑体光ストレス応答における役割 (2017)
  • Author(s): 林健太郎、癸生川奈央子、石井健雄、村山遙香、華岡光正
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
• [Presentation] 単細胞紅藻シゾンの概日時計応答に関わる細胞内シグナル伝達の解析 (2016)
  • Author(s): 片野貴章、雪竹健太郎、恵良厚子、宮城島進也、藤井岳、今村壮輔、田中寛、華岡光正
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] 単細胞紅藻C. merolaeの概日時計によるオルガネラへの情報伝達の解析 (2016)
  • Author(s): 片野貴章、雪竹健太郎、恵良厚子、宮城島進也、今村壮輔、田中寛、華岡光正
  • Organizer: 第15回微生物研究会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
• [Presentation] 葉緑体内の光環境変化に依存した核と葉緑体間の遺伝子発現協調 (2015)
  • Author(s): 安間美里、猪狩温、林健太郎、横山栞、齊藤光紀、鈴木雄介、江波和彦、華岡光正
  • Organizer: BMB2015（第38回日本分子生物学会年会・第88回日本生化学会大会合同大会）
  • Place of Presentation: 神戸国際展示場（兵庫県神戸市）
  • Year and Date: 2015-12-03
• [Presentation] Chloroplast-dependent regulation of nuclear gene expression in Arabidopsis thaliana. (2015)
  • Author(s): Misato Anma, Atsushi Ikari, Kentaro Hayashi, Shiori Yokoyama, Mitsunori Saito, Yusuke Suzuki, Kazuhiko Enami, and Mitumasa Hanaoka
  • Organizer: 2nd International Symposium "Matryoshka-type Evolution of Eukaryotic Cells"
  • Place of Presentation: 筑波大学会館（茨城県つくば市）
  • Year and Date: 2015-10-01
  • Int'l Joint Research
• [Presentation] 転写因子RpaA・RpaBの協調によるシアノバクテリアの概日時計出力系の制御 (2015)
  • Author(s): 徳山城永、小堀奈美、木村明日香、高井直樹、岩崎秀雄、近藤孝男、田中寛、華岡光正
  • Organizer: 第6回光合成学会・シンポジウム
  • Place of Presentation: 岡山国際交流センター（岡山県岡山市）
  • Year and Date: 2015-05-22
• [Presentation] CSK-dependent chloroplast light sensing in Arabidopsis thaliana (2015)
  • Author(s): Mitsunori Saito, Misato Anma, Shiori Yokoyama, Yusuke Suzuki, Kazuhiko Enami and Mitsumasa Hanaoka
  • Organizer: The 2nd International Symposium on Plant Environmental Sensing
  • Place of Presentation: 産業技術総合研究所（東京都江東区）
  • Year and Date: 2015-03-13
• [Presentation] Light sensing in chloroplasts (2015)
  • Author(s): Mitsumasa Hanaoka
  • Organizer: Tokyo Tech-HHU Dusseldorf Joint Symposium on Photosynthesis as a New Chemical Resource
  • Place of Presentation: 東京工業大学（東京都港区）
  • Year and Date: 2015-03-05
  • Invited
• [Presentation] Redox status regulates chloroplast transcription in the red algae Cyanidioschyzon merolae. (2015)
  • Author(s): Gaku Fujii, Sousuke Imamura, Keisuke Tarohara, Keisuke Yoshida, Toru Hisabori, Mitsumasa Hanaoka and Kan Tanaka
  • Organizer: Tokyo Tech-HHU Dusseldorf Joint Symposium on Photosynthesis as a New Chemical Resource
  • Place of Presentation: 東京工業大学（東京都港区）
  • Year and Date: 2015-03-04
• [Presentation] Plastid sigma factors and nucleus-chloroplast communication (2015)
  • Author(s): Mitsumasa Hanaoka
  • Organizer: Gordon Research Conference “Chloroplast Biotechnology: Reengineering Photosynthetic Organelles”
  • Place of Presentation: Ventura Beach Mariott, Ventura, CA, USA
  • Year and Date: 2015-01-19
  • Invited