Intracellular signaling machinery controlling endocytosis and uptake of extracellular materials
Project/Area Number |
26293041
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
General physiology
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Research Institution | Hokkaido University |
Principal Investigator |
Ohba Yusuke 北海道大学, 医学(系)研究科(研究院), 教授 (30333503)
|
Co-Investigator(Renkei-kenkyūsha) |
Nanbo Asuka 北海道大学, 大学院医学研究科, 准教授 (60359487)
Nishide Shinya 北海道大学, 大学院医学研究科, 助教 (40451398)
Fujioka Yoichiro 北海道大学, 大学院医学研究科, 助教 (70597492)
|
Research Collaborator |
Fujioka Mari
Horiuchi Kosui
Satoh Aya O.
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
|
Keywords | 蛍光バイオイメージング / エンドサイトーシス / シグナル伝達 / バイオイメージング / インフルエンザウイルス / 蛍光イメージング |
Outline of Final Research Achievements |
Endosome is a multifunctional platform through the emission and the regulation of a variety of signal transduction pathways. However, the relationship between signaling dynamics and cell function via the endosomes in living cells has yet to be analyzed in detail. In this study, we demonstrated that angiotensin II (AII) type 2 receptor (AT2R) negatively regulates type 1 receptor (AT1R)-signaling through a series of events including heterodimerization of AT1R and AT2R, internalization via endocytosis of the complex, change in the molecular orientation of receptors in the complex. We also clarified that activation of both receptors and and phosphorylation of serine residues at the C-terminus of AT2R by protein kinase C (PKC) are indispensable for this function.
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Rab5-regulated endocytosis plays a crucial role in apical extrusion of transformed cells.2017
Author(s)
S. Saitoh, T. Maruyama, Y. Yako, M. Kajita, Y. Fujioka, Y. Ohba, N. Kasai, N. Sugama, S. Kon, S. Ishikawa, T. Hayashi, T. Yamazaki, M. Tada and Y. Fujita.
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Journal Title
Proc. Natl. Acad. Sci. U.S.A.
Volume: 114
Issue: 12
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Improved FRET Biosensor for the Measurement of BCR-ABL Activity in Chronic Myeloid Leukemia Cells2017
Author(s)
M. Horiguchi, M. Fujioka, T. Kondo, Y. Fujioka, X. Li, K. Horiuchi, A.O. Satoh, P. Nepal, S. Nishide, A. Nanbo, T. Teshima and Y. Ohba.
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Journal Title
Cell Structure and Function
Volume: 42
Issue: 1
Pages: 15-26
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access
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[Journal Article] A leukemogenic kinase, FIP1L1-PDGFRA, and a SUMO E3 ligase, PIAS1, form a positive crosstalk via their enzymatic activities.2017
Author(s)
M. Ibata, J. Iwasaki, Y. Fujioka, K. Nakagawa, S. Darmanin, M. Onozawa, D. Hashimoto, Y. Ohba, S. Hatakeyama, T. Teshima and T. Kondo.
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Journal Title
Cancer Sci.
Volume: 108
Issue: 2
Pages: 200-207
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Tumour endothelial cells in high metastatic tumours promote metastasis via epigenetic dysregulation of biglycan.2016
Author(s)
N. Maishi, Y. Ohba, K. Akiyama, N. Ohga, J.I. Hamada, H. Nagao-Kitamoto, M. Alam, K. Yamamoto, T. Kawamoto, N. Inoue, A. Taketomi, M. Shindoh, Y. Hida and K. Hida.
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 28039-28039
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Lypd8 promotes the segregation of flagellated microbiota and colonic epithelia.2016
Author(s)
Okumura R, Kurakawa T, Nakano T, Kayama H, Kinoshita M, Motoka D, Gotoh K, Kimura T, Kamiyama N, Kusu T, Ueda Y, Wu H, Iijima H, Barman S, Osawwa H, Matsuno H, Nishimura J, Ohba Y, Nakamura S, Iida T, Yamamoto M, Umemoto E, Sano K, and Takeda K
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Journal Title
Nature
Volume: 532
Issue: 7597
Pages: 117-121
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Receptor activator of NF-κB ligand induces cell adhesion and integrin α2 expression via NF-κB in head and neck cancers2016
Author(s)
T.Yamada, M. Tsuda, T. Wagatsuma, Y. Fujioka, M. Fujioka, A. O. Satoh, K. Horiuchi, S. Nishide, A. Nanbo, Y. Totsuka, H. Haga, S. Tanaka, M. Shindoh, and Y. Ohba
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 23545-23545
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A role of the sphingosine-1-phosphate (S1P)- S1P receptor 2 pathway in Epithelial Defense Against Cancer (EDAC)2015
Author(s)
Yamamoto, S., Yako, Y., Fujioka, Y., Kajita, M., Kameyama, T., Kon, S., Ishikawa, S., Ohba, Y., Ohno, Y., Kihara, A., and Fujita, Y.
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Journal Title
Molecular Biology of the Cell
Volume: 27(3)
Issue: 3
Pages: 491-499
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] CRK adaptor protein induces epithelial-mesenchymal transition and metastasis of bladder cancer cells via HGF/c-Met feedback loop.2015
Author(s)
Matsumoto R, Tsuda M, Wang L, Maishi N, Abe t, Kimura T, Tanino M, Nishihara H, Hida K, Ohba Y, Shinohara N, Nonomura K, Tanaka S.
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Journal Title
Cancer Sci
Volume: in press
Issue: 6
Pages: 709-17
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Inhibition of multidrug transporter in tumor endothelial cells enhances antiangiogenic effects of low-dose metronomic paclitaxel2015
Author(s)
1.Akiyama K., Maishi N., Ohga N., Hida Y., Ohba Y., Alam Mohammad Towfik, Kawamoto T., Ohmura H., Yamada K., Torii C., Shindoh M. and *Hida K.
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Journal Title
Am J Pathol
Volume: 185(2)
Issue: 2
Pages: 572-580
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Agonist-promoted ubiquitination differentially regulates receptor trafficking of endothelin type A and type B receptors.2014
Author(s)
Terada K., Horinouchi T., Fujioka Y., Higashi T., Nepal P., Horiguchi M., Sarita K., Hatate C., Hoshi A., Harada T., Mai Y., Ohba Y. and Miwa S.
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 51
Pages: 35283-35295
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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